| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 232,901 | C→T | 10.0% | intergenic (‑140/+30) | hemB ← / ← ykiB | porphobilinogen synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 232,901 | 0 | C | T | 10.0% | 115.8 / 4.1 | 40 | intergenic (‑140/+30) | hemB/ykiB | porphobilinogen synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/18); new base T (2/2); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTT > Exported/232831‑232970 | gTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAAc > 1:843355/1‑71 (MQ=255) gTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACtt > 1:1746118/1‑70 (MQ=255) cgcgATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAATTTta > 1:835463/1‑70 (MQ=255) aCAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGa > 1:1191031/1‑70 (MQ=255) gaagaGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTatat < 1:1241264/59‑1 (MQ=255) aaTGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATAttttt > 1:1370422/1‑70 (MQ=255) gAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTtat < 1:2308883/47‑1 (MQ=255) aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAatat > 1:1139609/1‑61 (MQ=255) aaGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtt > 1:3444513/1‑71 (MQ=255) gagagGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCttta > 1:1461308/1‑71 (MQ=255) gagGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttatt > 1:246809/1‑71 (MQ=255) agGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttattt < 1:1921078/71‑1 (MQ=255) agGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttattt < 1:1105051/71‑1 (MQ=255) ggCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttattta > 1:314191/1‑71 (MQ=255) ggCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATACTTTTAATCtttattt < 1:900364/70‑1 (MQ=255) aGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGc > 1:2711472/1‑71 (MQ=255) gACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTg < 1:2844478/71‑1 (MQ=255) aCATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGt > 1:3258840/1‑71 (MQ=255) cATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGtt > 1:1614413/1‑71 (MQ=255) cATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGtt < 1:3153533/71‑1 (MQ=255) aTCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAatat > 1:3398298/1‑46 (MQ=255) cTTTTTAATAGAAACAGCAATAATTTTATATTCACTGAAAATAttt > 1:3369744/1‑46 (MQ=255) tttAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGttat > 1:3445995/1‑71 (MQ=255) aTAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGttatta < 1:1526346/69‑1 (MQ=255) tAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCtttatt < 1:1135481/50‑1 (MQ=255) gAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttttt > 1:3036537/1‑71 (MQ=255) gAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttttt < 1:2739729/71‑1 (MQ=255) gAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTAttttt < 1:1582520/71‑1 (MQ=255) aaCAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGttatt > 1:3066173/1‑63 (MQ=255) aaCAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGttat > 1:2232436/1‑62 (MQ=255) cAGCAATAATTTTATATTCACTGAAAATATTTTTa < 1:2783965/35‑1 (MQ=255) cAGCAATAATTTTATATTCACTGAAAATATTTTTa < 1:2662108/35‑1 (MQ=255) aGCAATAACTTTATATTCACTGTAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAg < 1:3617960/71‑1 (MQ=255) gCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGt < 1:3024746/71‑1 (MQ=255) cAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGtt > 1:2444217/1‑71 (MQ=255) cAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGtt < 1:806604/71‑1 (MQ=255) aaTAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGtt < 1:1269262/70‑1 (MQ=255) tAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTgg < 1:785825/71‑1 (MQ=255) aCTTTATATTCACTGAAAATATTTTTAATCTttatttat > 1:1723124/1‑39 (MQ=255) aCTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTgg < 1:2454692/69‑1 (MQ=255) aCTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGtt < 1:1362992/71‑1 (MQ=255) | GTTGTCGCGATGTTGACAAGAAGAGAATGGAAGAGAGGCCAGGACATCTTTTTAATAGAAACAGCAATAACTTTATATTCACTGAAAATATTTTTAATCTTTATTTATAGCTGTTGGTTATTATTTTTTGGAGTTTGGTT > Exported/232831‑232970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |