Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,523,766 T→A 8.5% intergenic (‑52/+169) dinD ← / ← yicC DNA‑damage‑inducible protein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,523,7660TA 8.5% 232.3 / 11.8 82intergenic (‑52/+169)dinD/yicCDNA‑damage‑inducible protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (31/44);  new base A (2/5);  total (33/49)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

GATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGC  >  Exported/2523699‑2523833
                                                                   |                                                                   
gatgatGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTTTTta                                                                   <  1:970881/70‑1 (MQ=255)
 atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtat                                                                  <  1:2217462/70‑1 (MQ=255)
 atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta                                                                   <  1:2426353/69‑1 (MQ=255)
    atgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata                                                               >  1:2941267/1‑70 (MQ=255)
      gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACa                                                             <  1:2632991/70‑1 (MQ=255)
        tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt                                                          >  1:2148883/1‑71 (MQ=255)
         cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt                                                          <  1:984556/70‑1 (MQ=255)
         cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt                                                          <  1:2154907/70‑1 (MQ=255)
          gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta                                                         <  1:1225215/70‑2 (MQ=39)
          gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta                                                         <  1:3629899/70‑2 (MQ=39)
            tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt                                                        >  1:3386730/1‑69 (MQ=255)
              aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt                                                    <  1:1169905/71‑1 (MQ=255)
               ttGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa                                                   <  1:3317848/71‑1 (MQ=255)
                 gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt                                                  <  1:3059117/70‑1 (MQ=255)
                  cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAtt                                                                     <  1:3319974/50‑1 (MQ=255)
                    cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTta                                                                   >  1:888666/1‑50 (MQ=255)
                    cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa                                              >  1:1871086/1‑71 (MQ=255)
                     aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc                                             <  1:2834017/71‑1 (MQ=255)
                      cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt                                                    <  1:961526/63‑1 (MQ=255)
                      cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg                                            >  1:405978/1‑71 (MQ=255)
                       tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:2235799/65‑1 (MQ=255)
                       tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGt                                           >  1:659584/1‑71 (MQ=255)
                        ccATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt                                          >  1:2804320/1‑71 (MQ=255)
                         cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa                                                      >  1:2524385/1‑58 (MQ=255)
                         cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa                                                      >  1:814682/1‑58 (MQ=255)
                          aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt                                          <  1:1319660/69‑1 (MQ=255)
                             tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa                                      <  1:531394/70‑1 (MQ=255)
                             tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAg                                     >  1:236335/1‑71 (MQ=255)
                                tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt                                  <  1:382091/71‑1 (MQ=255)
                                tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt                                  <  1:1411151/71‑1 (MQ=255)
                                  tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg                                 <  1:1810801/70‑1 (MQ=255)
                                  tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc                                >  1:3204743/1‑71 (MQ=255)
                                  tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc                                <  1:783687/71‑1 (MQ=255)
                                    aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt                                                  >  1:1521347/1‑51 (MQ=255)
                                    aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg                              <  1:1599276/71‑1 (MQ=255)
                                    aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg                              <  1:3644584/71‑1 (MQ=255)
                                     aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                             >  1:577004/1‑71 (MQ=255)
                                     aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                             <  1:3555545/71‑1 (MQ=255)
                                     aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                             <  1:1987130/71‑1 (MQ=255)
                                         cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:336324/47‑1 (MQ=255)
                                         cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:2414842/47‑1 (MQ=255)
                                         cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:1184437/47‑1 (MQ=255)
                                         cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:3419971/47‑1 (MQ=255)
                                         cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa                         <  1:950644/71‑1 (MQ=255)
                                          aaagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 <  1:2907022/46‑1 (MQ=255)
                                           aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                               >  1:2892285/1‑47 (MQ=255)
                                           aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                               >  1:131689/1‑47 (MQ=255)
                                           aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                               >  1:2962078/1‑47 (MQ=255)
                                           aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc                                         <  1:970266/53‑1 (MQ=255)
                                            agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                 >  1:1796667/1‑44 (MQ=255)
                                            agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                      <  1:1543452/71‑1 (MQ=255)
                                             gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                       >  1:99285/1‑53 (MQ=255)
                                                agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                      <  1:3560956/67‑1 (MQ=255)
                                                agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAAc                   <  1:3593340/70‑1 (MQ=255)
                                                agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                  <  1:702274/71‑1 (MQ=255)
                                                 gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                       <  1:1425078/49‑1 (MQ=255)
                                                 gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                       <  1:668119/49‑1 (MQ=255)
                                                  tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg                                            >  1:2673919/1‑43 (MQ=255)
                                                  tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                  <  1:1266355/69‑1 (MQ=255)
                                                  tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                  <  1:1421635/69‑1 (MQ=255)
                                                  tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATc                >  1:2211912/1‑71 (MQ=255)
                                                    aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa                     >  1:210656/1‑64 (MQ=255)
                                                    aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACAt                 >  1:1274397/1‑68 (MQ=255)
                                                     aTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGc              >  1:2134342/1‑70 (MQ=255)
                                                       cTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGTGCaaaaa                      <  1:1611939/60‑1 (MQ=255)
                                                         gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                       <  1:3706986/41‑1 (MQ=255)
                                                         gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa                    >  1:3156716/1‑60 (MQ=255)
                                                          tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa           <  1:3589877/68‑1 (MQ=255)
                                                          tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa            <  1:1905052/67‑1 (MQ=255)
                                                          tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAAt         <  1:2606890/70‑1 (MQ=255)
                                                          tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATc        <  1:1781716/71‑1 (MQ=255)
                                                           aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaa                        <  1:788235/54‑1 (MQ=255)
                                                           aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCt       >  1:895887/1‑71 (MQ=255)
                                                           aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg                            >  1:1619997/1‑50 (MQ=38)
                                                           aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg                            >  1:2892083/1‑50 (MQ=38)
                                                             cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                      >  1:1274413/1‑54 (MQ=255)
                                                             cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                      >  1:733466/1‑54 (MQ=255)
                                                             cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg      >  1:2025155/1‑70 (MQ=255)
                                                              tGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg      >  1:3032999/1‑69 (MQ=255)
                                                               gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt                               <  1:2995203/43‑1 (MQ=255)
                                                               gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt                               <  1:3362744/43‑1 (MQ=255)
                                                               gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg   >  1:2788934/1‑71 (MQ=255)
                                                                tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGc  <  1:1465300/71‑1 (MQ=255)
                                                                   |                                                                   
GATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGC  >  Exported/2523699‑2523833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: