Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,523,769 | T→A | 9.1% | intergenic (‑55/+166) | dinD ← / ← yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,523,769 | 0 | T | A | 9.1% | 216.1 / 12.0 | 77 | intergenic (‑55/+166) | dinD/yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
Reads supporting (aligned to +/- strand): ref base T (29/41); new base A (2/5); total (31/46) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.95e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 |
ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAC > Exported/2523700‑2523839 | atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtat < 1:2217462/70‑1 (MQ=255) atgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata > 1:2941267/1‑70 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACa < 1:2632991/70‑1 (MQ=255) tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt > 1:2148883/1‑71 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt < 1:984556/70‑1 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt < 1:2154907/70‑1 (MQ=255) gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta < 1:1225215/70‑2 (MQ=39) gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta < 1:3629899/70‑2 (MQ=39) tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt > 1:3386730/1‑69 (MQ=255) aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt < 1:1169905/71‑1 (MQ=255) ttGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa < 1:3317848/71‑1 (MQ=255) gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt < 1:3059117/70‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa > 1:1871086/1‑71 (MQ=255) aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:2834017/71‑1 (MQ=255) cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt < 1:961526/63‑1 (MQ=255) cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg > 1:405978/1‑71 (MQ=255) tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2235799/65‑1 (MQ=255) tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGt > 1:659584/1‑71 (MQ=255) ccATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt > 1:2804320/1‑71 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa > 1:2524385/1‑58 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa > 1:814682/1‑58 (MQ=255) aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:1319660/69‑1 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa < 1:531394/70‑1 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAg > 1:236335/1‑71 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt < 1:1411151/71‑1 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt < 1:382091/71‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg < 1:1810801/70‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc > 1:3204743/1‑71 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc < 1:783687/71‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt > 1:1521347/1‑51 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg < 1:3644584/71‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg < 1:1599276/71‑1 (MQ=255) aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg < 1:1987130/71‑1 (MQ=255) aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg > 1:577004/1‑71 (MQ=255) aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg < 1:3555545/71‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:336324/47‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:3419971/47‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:1184437/47‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2414842/47‑1 (MQ=255) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa < 1:950644/71‑1 (MQ=255) aaagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2907022/46‑1 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc > 1:131689/1‑47 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc > 1:2892285/1‑47 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc > 1:2962078/1‑47 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc < 1:970266/53‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc > 1:1796667/1‑44 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:1543452/71‑1 (MQ=255) gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc > 1:99285/1‑53 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:3560956/67‑1 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAAc < 1:3593340/70‑1 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:702274/71‑1 (MQ=255) gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc < 1:668119/49‑1 (MQ=255) gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc < 1:1425078/49‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg > 1:2673919/1‑43 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:1266355/69‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:1421635/69‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATc > 1:2211912/1‑71 (MQ=255) aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa > 1:210656/1‑64 (MQ=255) aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACAt > 1:1274397/1‑68 (MQ=255) aTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGc > 1:2134342/1‑70 (MQ=255) cTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGTGCaaaaa < 1:1611939/60‑1 (MQ=255) gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc < 1:3706986/41‑1 (MQ=255) gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa > 1:3156716/1‑60 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa < 1:3589877/68‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa < 1:1905052/67‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAAt < 1:2606890/70‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATc < 1:1781716/71‑1 (MQ=255) aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaa < 1:788235/54‑1 (MQ=255) aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCt > 1:895887/1‑71 (MQ=255) aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg > 1:2892083/1‑50 (MQ=38) aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg > 1:1619997/1‑50 (MQ=38) cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa > 1:733466/1‑54 (MQ=255) cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa > 1:1274413/1‑54 (MQ=255) cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg > 1:2025155/1‑70 (MQ=255) tGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg > 1:3032999/1‑69 (MQ=255) gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:2995203/43‑1 (MQ=255) gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:3362744/43‑1 (MQ=255) gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg > 1:2788934/1‑71 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGc < 1:1465300/71‑1 (MQ=255) atataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGc < 1:2458240/70‑1 (MQ=255) atataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCa > 1:1348496/1‑71 (MQ=255) tataCACTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAc > 1:1904876/1‑71 (MQ=255) | ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAC > Exported/2523700‑2523839 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |