Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,523,769 T→A 9.1% intergenic (‑55/+166) dinD ← / ← yicC DNA‑damage‑inducible protein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,523,7690TA 9.1% 216.1 / 12.0 77intergenic (‑55/+166)dinD/yicCDNA‑damage‑inducible protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (29/41);  new base A (2/5);  total (31/46)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAC  >  Exported/2523700‑2523839
                                                                     |                                                                      
atgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtat                                                                        <  1:2217462/70‑1 (MQ=255)
   atgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata                                                                     >  1:2941267/1‑70 (MQ=255)
     gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACa                                                                   <  1:2632991/70‑1 (MQ=255)
       tcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt                                                                >  1:2148883/1‑71 (MQ=255)
        cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt                                                                <  1:984556/70‑1 (MQ=255)
        cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtt                                                                <  1:2154907/70‑1 (MQ=255)
         gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta                                                               <  1:1225215/70‑2 (MQ=39)
         gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta                                                               <  1:3629899/70‑2 (MQ=39)
           tcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt                                                              >  1:3386730/1‑69 (MQ=255)
             aTTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt                                                          <  1:1169905/71‑1 (MQ=255)
              ttGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTa                                                         <  1:3317848/71‑1 (MQ=255)
                gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt                                                        <  1:3059117/70‑1 (MQ=255)
                   cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa                                                    >  1:1871086/1‑71 (MQ=255)
                    aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc                                                   <  1:2834017/71‑1 (MQ=255)
                     cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt                                                          <  1:961526/63‑1 (MQ=255)
                     cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg                                                  >  1:405978/1‑71 (MQ=255)
                      tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:2235799/65‑1 (MQ=255)
                      tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGt                                                 >  1:659584/1‑71 (MQ=255)
                       ccATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt                                                >  1:2804320/1‑71 (MQ=255)
                        cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa                                                            >  1:2524385/1‑58 (MQ=255)
                        cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGa                                                            >  1:814682/1‑58 (MQ=255)
                         aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt                                                <  1:1319660/69‑1 (MQ=255)
                            tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa                                            <  1:531394/70‑1 (MQ=255)
                            tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAg                                           >  1:236335/1‑71 (MQ=255)
                               tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt                                        <  1:1411151/71‑1 (MQ=255)
                               tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt                                        <  1:382091/71‑1 (MQ=255)
                                 tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg                                       <  1:1810801/70‑1 (MQ=255)
                                 tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc                                      >  1:3204743/1‑71 (MQ=255)
                                 tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGc                                      <  1:783687/71‑1 (MQ=255)
                                   aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt                                                        >  1:1521347/1‑51 (MQ=255)
                                   aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg                                    <  1:3644584/71‑1 (MQ=255)
                                   aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg                                    <  1:1599276/71‑1 (MQ=255)
                                    aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                                   <  1:1987130/71‑1 (MQ=255)
                                    aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                                   >  1:577004/1‑71 (MQ=255)
                                    aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg                                   <  1:3555545/71‑1 (MQ=255)
                                        cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:336324/47‑1 (MQ=255)
                                        cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:3419971/47‑1 (MQ=255)
                                        cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:1184437/47‑1 (MQ=255)
                                        cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:2414842/47‑1 (MQ=255)
                                        cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa                               <  1:950644/71‑1 (MQ=255)
                                         aaagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       <  1:2907022/46‑1 (MQ=255)
                                          aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                                     >  1:131689/1‑47 (MQ=255)
                                          aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                                     >  1:2892285/1‑47 (MQ=255)
                                          aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtctc                                                     >  1:2962078/1‑47 (MQ=255)
                                          aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtc                                               <  1:970266/53‑1 (MQ=255)
                                           agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc                                                       >  1:1796667/1‑44 (MQ=255)
                                           agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                            <  1:1543452/71‑1 (MQ=255)
                                            gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                             >  1:99285/1‑53 (MQ=255)
                                               agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                            <  1:3560956/67‑1 (MQ=255)
                                               agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAAc                         <  1:3593340/70‑1 (MQ=255)
                                               agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                        <  1:702274/71‑1 (MQ=255)
                                                gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                             <  1:668119/49‑1 (MQ=255)
                                                gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                             <  1:1425078/49‑1 (MQ=255)
                                                 tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACg                                                  >  1:2673919/1‑43 (MQ=255)
                                                 tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                        <  1:1266355/69‑1 (MQ=255)
                                                 tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa                        <  1:1421635/69‑1 (MQ=255)
                                                 tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATc                      >  1:2211912/1‑71 (MQ=255)
                                                   aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa                           >  1:210656/1‑64 (MQ=255)
                                                   aaTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACAt                       >  1:1274397/1‑68 (MQ=255)
                                                    aTCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGc                    >  1:2134342/1‑70 (MQ=255)
                                                      cTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGTGCaaaaa                            <  1:1611939/60‑1 (MQ=255)
                                                        gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtctc                                             <  1:3706986/41‑1 (MQ=255)
                                                        gTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa                          >  1:3156716/1‑60 (MQ=255)
                                                         tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa                 <  1:3589877/68‑1 (MQ=255)
                                                         tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa                  <  1:1905052/67‑1 (MQ=255)
                                                         tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAAt               <  1:2606890/70‑1 (MQ=255)
                                                         tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATc              <  1:1781716/71‑1 (MQ=255)
                                                          aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaa                              <  1:788235/54‑1 (MQ=255)
                                                          aaCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCt             >  1:895887/1‑71 (MQ=255)
                                                          aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg                                  >  1:2892083/1‑50 (MQ=38)
                                                          aaCTGTATATAAATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg                                  >  1:1619997/1‑50 (MQ=38)
                                                            cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                            >  1:733466/1‑54 (MQ=255)
                                                            cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa                            >  1:1274413/1‑54 (MQ=255)
                                                            cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg            >  1:2025155/1‑70 (MQ=255)
                                                             tGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTg            >  1:3032999/1‑69 (MQ=255)
                                                              gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt                                     <  1:2995203/43‑1 (MQ=255)
                                                              gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt                                     <  1:3362744/43‑1 (MQ=255)
                                                              gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAg         >  1:2788934/1‑71 (MQ=255)
                                                               tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGc        <  1:1465300/71‑1 (MQ=255)
                                                                    atataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGc    <  1:2458240/70‑1 (MQ=255)
                                                                    atataCAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCa   >  1:1348496/1‑71 (MQ=255)
                                                                     tataCACTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAc  >  1:1904876/1‑71 (MQ=255)
                                                                     |                                                                      
ATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCAGGCAC  >  Exported/2523700‑2523839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: