| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,546,001 | C→T | 51.6% | M182I (ATG→ATA) | rfaF ← | ADP‑heptose:LPS heptosyltransferase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,546,001 | 0 | C | T | 51.6% | 2.1 / 88.3 | 62 | M182I (ATG→ATA) | rfaF | ADP‑heptose:LPS heptosyltransferase II |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base T (19/13); total (34/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGCAACT > Exported/2545932‑2546071 | cAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatca < 1:3727724/71‑1 (MQ=255) gCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcatc < 1:2405497/71‑1 (MQ=255) gCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcatc > 1:1228174/1‑71 (MQ=255) gCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAatcatc < 1:2618343/71‑1 (MQ=255) cTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCg > 1:2379149/1‑71 (MQ=255) cTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCg > 1:3677500/1‑71 (MQ=255) ccGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCg > 1:1458157/1‑69 (MQ=255) gCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc > 1:3243920/1‑66 (MQ=255) gCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc > 1:1691740/1‑66 (MQ=255) gCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAattatc > 1:1302510/1‑66 (MQ=255) gCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACg > 1:119903/1‑71 (MQ=255) gCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACg > 1:543173/1‑71 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCg < 1:1336456/58‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCg < 1:1754213/58‑1 (MQ=255) tAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatg > 1:1812829/1‑70 (MQ=255) aGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga > 1:1567002/1‑70 (MQ=255) gtgtGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCtgatga > 1:2437705/1‑69 (MQ=255) gCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCg < 1:1341541/69‑1 (MQ=255) cAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGaa < 1:1715245/63‑1 (MQ=255) cAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGaaaa > 1:1454600/1‑71 (MQ=255) gCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAAtt < 1:325238/71‑1 (MQ=255) ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACg < 1:3448072/48‑1 (MQ=255) ttttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACg < 1:2152974/48‑1 (MQ=255) tttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACg < 1:721953/47‑1 (MQ=255) tttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:559022/1‑70 (MQ=255) tttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:609926/1‑70 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGAtt < 1:2778650/70‑1 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:2103461/1‑71 (MQ=255) ttGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:1680317/1‑71 (MQ=255) tGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCgg < 1:3199684/42‑1 (MQ=255) tGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCtga > 1:3205979/1‑51 (MQ=255) tGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCtga > 1:313233/1‑51 (MQ=255) tGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:2096905/70‑1 (MQ=255) gCCGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGTACGTTCTGATGAAAGCTAAAATTGATTAc < 1:562090/70‑1 (MQ=255) gCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:2690928/1‑69 (MQ=255) cGGACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGa > 1:1980364/1‑64 (MQ=255) ggACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGaaa > 1:1766941/1‑52 (MQ=255) gACCAAACTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGaaa > 1:1378598/1‑51 (MQ=255) aCCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGtat < 1:942223/71‑1 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa < 1:1229687/71‑1 (MQ=255) aaCTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGa < 1:1488498/71‑1 (MQ=255) aCTCCGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAt > 1:1300823/1‑71 (MQ=255) cGCACCCGGGCAAAAACCAATTATCGGACGTTCtgatga < 1:1824593/39‑1 (MQ=255) cGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:3017481/1‑53 (MQ=255) cGCACCCGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:3377111/1‑53 (MQ=255) cGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAttttt < 1:1597963/71‑1 (MQ=255) cACCCGGGAAAAAACCAATTATCGGACGTTCTGATGAAAGCGaaa > 1:118626/1‑45 (MQ=255) aCCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCa > 1:3502979/1‑70 (MQ=255) cccGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCAcc < 1:479825/71‑1 (MQ=255) ccGGGCAAAAACCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCt > 1:68524/1‑71 (MQ=255) cGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCtt < 1:2764852/71‑1 (MQ=255) gggCAAAAACCAATCATCGGACGTTCTGATGAAAGGGAAAATTGATTa > 1:3582537/1‑48 (MQ=255) ggCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCg > 1:1240442/1‑71 (MQ=255) aaaaaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTc < 1:3324193/71‑1 (MQ=255) aaaaaCCAATCATCGGACGTTCTGATGAAAGCGaa < 1:1646994/35‑1 (MQ=255) aaaCCAATTATCGGACGTTCTGATGAAAGCGAAAAtt < 1:303495/37‑1 (MQ=255) aaaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc > 1:1952523/1‑71 (MQ=255) aaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGAt > 1:3308546/1‑40 (MQ=255) aaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa > 1:2200642/1‑42 (MQ=255) aaaCCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCAc < 1:3168199/71‑1 (MQ=255) aaCCAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:2391705/41‑1 (MQ=255) ccAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACa > 1:562778/1‑41 (MQ=255) ccAATTATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTg > 1:3235689/1‑71 (MQ=255) ccAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:1945129/39‑1 (MQ=255) ccAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:1905310/39‑1 (MQ=255) cAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:2828444/38‑1 (MQ=255) cAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCt > 1:1803228/1‑69 (MQ=255) cAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCt > 1:1291959/1‑69 (MQ=255) aaTCATCGGACGTTCTGATGAAAGCGAAAATTGATTa < 1:2338951/37‑1 (MQ=255) catcGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGAtttt > 1:2041530/1‑49 (MQ=255) catcGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGCAACt > 1:18823/1‑71 (MQ=255) | CAGCTCCGCATAGTGGTAGTGTGGCCAGCGTTTTGCCGGACCAAACTCCGCACCCGGGCAAAAACCAATCATCGGACGTTCTGATGAAAGCGAAAATTGATTACAGGTATATGATTTTTCACCTTCGCTCACCTGCAACT > Exported/2545932‑2546071 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |