| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,547,976 | T→C | 10.2% | V62A (GTT→GCT) | htrL → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,547,976 | 0 | T | C | 10.2% | 152.7 / 9.3 | 59 | V62A (GTT→GCT) | htrL | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (23/30); new base C (3/3); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.23e-01 | |||||||||||
TACTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGATATAT > Exported/2547906‑2548045 | tACTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGt > 1:1521461/1‑71 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:2299004/71‑1 (MQ=255) cTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:1199100/71‑1 (MQ=255) ttGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg > 1:920740/1‑69 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGa < 1:1534327/69‑1 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAg > 1:1963337/1‑71 (MQ=255) tGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAg > 1:1163719/1‑71 (MQ=255) gAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGa < 1:1093411/68‑1 (MQ=255) aGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCg < 1:1999098/71‑1 (MQ=255) gCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGa < 1:1792169/71‑1 (MQ=255) gTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGAtt > 1:3208507/1‑71 (MQ=255) gTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGAtt < 1:1127940/71‑1 (MQ=255) gCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCg < 1:3248024/68‑1 (MQ=255) gCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCg < 1:1004863/68‑1 (MQ=255) gCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCaa < 1:79609/71‑1 (MQ=255) gCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCaa < 1:506011/71‑1 (MQ=255) ccGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGaa > 1:1134183/1‑59 (MQ=255) ccGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCa < 1:2833034/69‑1 (MQ=255) cGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACg > 1:2923626/1‑71 (MQ=255) aCTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTg < 1:3624474/53‑1 (MQ=255) aCTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGa > 1:2639375/1‑70 (MQ=255) tGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGaaaa > 1:251755/1‑70 (MQ=255) aaaaTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAAcc < 1:2485127/70‑1 (MQ=255) aaaTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaa > 1:688336/1‑71 (MQ=255) aTGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaaca < 1:2350586/71‑1 (MQ=255) tGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaacaa < 1:1317088/71‑1 (MQ=255) tGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACcaacaa < 1:1771488/71‑1 (MQ=255) gatgatTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGaa < 1:1303391/59‑1 (MQ=255) attattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGc < 1:2051976/41‑1 (MQ=255) ttattTTCACTTCTCCCGATTTGAAACCAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGgttatc > 1:1087910/1‑71 (MQ=255) ttattTTCACTTCTCCCGATTTGAAACAAAGGGCTGAAGCGATTCGCAACGGAAAACCAACAACGgttat > 1:3713881/1‑70 (MQ=255) tattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAAc > 1:2304969/1‑49 (MQ=255) tattTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAAc > 1:399265/1‑49 (MQ=255) ttttCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgtt < 1:2274495/71‑1 (MQ=255) aCTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGttatcgttatcg < 1:2917412/70‑1 (MQ=255) aCTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGttatcgttatcg < 1:2976768/70‑1 (MQ=255) cTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGaa < 1:2094215/45‑1 (MQ=255) ctcCCGATTTGAAACCAAGGGTTGAAGCGATTCGCaa > 1:2286581/1‑37 (MQ=255) ctcCCGATTTGAAACCAAGGGTTGAAGCGATTCGCaa > 1:2809559/1‑37 (MQ=255) cccGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaa > 1:3235385/1‑70 (MQ=255) ccGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaa < 1:1104372/70‑1 (MQ=255) aTTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATaaaa > 1:1517563/1‑68 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGaaa > 1:3675307/1‑67 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGaaa > 1:1535055/1‑67 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTa < 1:520090/71‑1 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTa < 1:3706059/71‑1 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTa < 1:174264/71‑1 (MQ=255) aCCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTa < 1:1721465/71‑1 (MQ=255) ccAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAAtt > 1:108095/1‑68 (MQ=255) ccAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAg < 1:502940/71‑1 (MQ=255) aaGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatc > 1:3081744/1‑43 (MQ=255) aaGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatc > 1:1744794/1‑43 (MQ=255) aaGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGaa < 1:538062/63‑1 (MQ=255) gggTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGata > 1:1858329/1‑70 (MQ=255) gggTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGata > 1:1475894/1‑70 (MQ=255) ggTTGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgttat < 1:3239907/45‑1 (MQ=255) ggTTGAAGCGATTCGCAACGGAAAACCAACAACGGttatcgttatcg > 1:2680820/1‑47 (MQ=255) ggTTGAAGCGATTCGCAACGGAAAACCAACAACGGttatcgttatcg > 1:2490042/1‑47 (MQ=255) gTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGatatat < 1:1924018/71‑1 (MQ=255) gTTGAAGCGATTCGCAACGGAAAA‑CAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGatatat < 1:961313/70‑1 (MQ=255) ttGAAGCGATTCGCAACGGAAAACCAACAACGgttatcgtt < 1:2436147/41‑1 (MQ=255) ttGAAGCGATTCGCAACGGAAAACCAACAACGgttatc < 1:2742607/38‑1 (MQ=255) | TACTTTGAGCGTCTGGCCGCACTTGAAAATGAGATGATTATTTTCACTTCTCCCGATTTGAAACCAAGGGTTGAAGCGATTCGCAACGGAAAACCAACAACGGTTATCGTTATCGATATAAAAAAGAAATTTAGATATAT > Exported/2547906‑2548045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |