| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,550,360 | A→G | 52.9% | K77K (AAA→AAG) | tdh → | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,550,360 | 0 | A | G | 52.9% | 17.6 / 148.6 | 104 | K77K (AAA→AAG) | tdh | threonine 3‑dehydrogenase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base A (28/21); new base G (28/27); total (56/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > Exported/2550293‑2550430 | tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3208855/1‑71 (MQ=255)tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2109575/1‑71 (MQ=255)tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3668368/1‑71 (MQ=255)tCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:3020075/1‑71 (MQ=255) cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2536461/1‑70 (MQ=255) cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2459228/1‑70 (MQ=255) cccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGc > 1:2759388/1‑70 (MQ=255) ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa > 1:2794998/1‑66 (MQ=255) ccGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCt > 1:1479506/1‑70 (MQ=255) tGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAg < 1:366746/71‑1 (MQ=255) tGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAg < 1:1779985/71‑1 (MQ=255) cGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTc > 1:3711232/1‑65 (MQ=255) tGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCgg > 1:2473631/1‑70 (MQ=255) ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGAt < 1:815201/66‑1 (MQ=255) ggTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGc < 1:1118491/70‑1 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:3181797/1‑71 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1589485/1‑71 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1443340/1‑71 (MQ=255) tCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGAt > 1:1121668/1‑71 (MQ=255) ggCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt > 1:3313833/1‑71 (MQ=255) gCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGtt < 1:2779441/71‑1 (MQ=255) ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTc < 1:2218290/52‑1 (MQ=255) ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGttt > 1:3030241/1‑71 (MQ=255) ccATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGttt < 1:1873399/71‑1 (MQ=255) cATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTc < 1:2376132/71‑1 (MQ=255) aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:2905938/71‑1 (MQ=255) aTGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCt < 1:1551168/71‑1 (MQ=255) tGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGaaa < 1:2962287/43‑1 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2906789/1‑71 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2907734/1‑71 (MQ=255) gAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg > 1:2611229/1‑71 (MQ=255) aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAgg > 1:3112565/1‑43 (MQ=255) aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:1525019/70‑1 (MQ=255) aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:483153/70‑1 (MQ=255) aaTATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:1266625/1‑71 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:951726/1‑70 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:1063634/1‑70 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:376162/1‑70 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:349740/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:3206729/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:278192/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:1032341/71‑1 (MQ=255) atatGTCGGTGAAGTGGTAGGGATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGc > 1:2414956/1‑70 (MQ=255) tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:1253636/70‑1 (MQ=255) tGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa < 1:2540188/70‑1 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCtt > 1:3099619/1‑41 (MQ=255) gTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCtt > 1:836521/1‑41 (MQ=255) cGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:2335652/65‑1 (MQ=255) ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTgg < 1:647997/62‑1 (MQ=255) ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:3033450/1‑50 (MQ=255) ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:2628434/1‑50 (MQ=255) ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGa > 1:2180549/1‑50 (MQ=255) ggTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:1935471/71‑1 (MQ=255) tGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:1910743/69‑1 (MQ=255) gAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCa < 1:107102/68‑1 (MQ=255) aGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:3112115/1‑71 (MQ=255) aGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCa > 1:2101227/1‑71 (MQ=255) aGTGGGAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCg < 1:972241/41‑1 (MQ=38) gTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGa > 1:2362027/1‑37 (MQ=255) gTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGAt > 1:2779210/1‑38 (MQ=255) tGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:1062790/1‑59 (MQ=255) tGGTAGGTATTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:2112563/1‑59 (MQ=255) tGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGGGATCGCGTTTCTGGCGAAGGCCATATCAcc < 1:556714/71‑1 (MQ=255) gtaggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtg > 1:1095541/1‑71 (MQ=255) gtaggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCt < 1:3235005/70‑1 (MQ=255) aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:1411662/61‑1 (MQ=255) aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:2461949/61‑1 (MQ=255) aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCat < 1:1319953/61‑1 (MQ=255) aggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg > 1:1968076/1‑71 (MQ=255) ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:172018/44‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:913117/44‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGt < 1:2747493/44‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2542736/70‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2864806/70‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:717331/70‑1 (MQ=255) ggtaTTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCtgtg < 1:2417128/70‑1 (MQ=255) taTTGGTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTc < 1:1438877/71‑1 (MQ=255) tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCAcc > 1:291177/1‑61 (MQ=255) tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:2273538/1‑71 (MQ=255) tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:3508681/1‑71 (MQ=255) tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:1652872/1‑71 (MQ=255) tGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGTCATATCACCTGTGGTCAtt > 1:3044974/1‑71 (MQ=255) ggTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:2475803/1‑71 (MQ=255) ggTCAGGAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg > 1:1326206/1‑71 (MQ=255) ggTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGcc > 1:3247718/1‑52 (MQ=255) gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTg < 1:2901054/70‑1 (MQ=255) gTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGc < 1:2671026/71‑1 (MQ=255) tCAGGAAGTGAAGGGCTTCAAGATCGGAGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGcc < 1:3462161/71‑1 (MQ=255) tCAGGAAGTGAAAGGCTTCAAGATCGGCGATcgcg > 1:401675/1‑35 (MQ=255) tCAGGAAGTGAAAGGCTTCAAGATCGGCGATcgcg > 1:3065968/1‑35 (MQ=255) tCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGcc < 1:3095215/50‑1 (MQ=255) tCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGcc > 1:2797887/1‑71 (MQ=255) cAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCg > 1:378391/1‑71 (MQ=255) aGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc < 1:1671248/71‑1 (MQ=255) ggAAGTGAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGc > 1:1892454/1‑70 (MQ=255) gAAGGGCTTCAAGATCGGCGATCGCGTTTCTGGCg < 1:948385/35‑1 (MQ=255) aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGaa > 1:896292/1‑36 (MQ=255) aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:475498/1‑71 (MQ=255) aaaGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCg > 1:2746950/1‑71 (MQ=255) aGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCgtg > 1:1022705/1‑71 (MQ=255) | TCCCGGTGCCGATGGTCGTGGGCCATGAATATGTCGGTGAAGTGGTAGGTATTGGTCAGGAAGTGAAAGGCTTCAAGATCGGCGATCGCGTTTCTGGCGAAGGCCATATCACCTGTGGTCATTGCCGCAACTGTCGTG > Exported/2550293‑2550430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |