Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,581,803 T→G 5.3% intergenic (+293/‑77) gadA → / → gadX glutamate decarboxylase A, PLP‑dependent/DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,581,8030TG 5.3% 212.7 / 5.6 76intergenic (+293/‑77)gadA/gadXglutamate decarboxylase A, PLP‑dependent/DNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (35/37);  new base G (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.51e-01

AATCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAATATT  >  Exported/2581734‑2581870
                                                                     |                                                                   
aaTCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAatttattt                                                                     <  1:939819/70‑1 (MQ=255)
 aTCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATttatttat                                                                   <  1:3205585/71‑1 (MQ=255)
 aTCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATttatttat                                                                   <  1:895811/71‑1 (MQ=255)
 aTCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATttatttat                                                                   <  1:68060/71‑1 (MQ=255)
    cAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaa                                                                <  1:3229019/71‑1 (MQ=255)
    cAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaa                                                                <  1:2771824/71‑1 (MQ=255)
    cAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatca                                                                 <  1:40766/70‑1 (MQ=255)
     aGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaat                                                               <  1:1216110/71‑1 (MQ=255)
      gTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaat                                                               >  1:2574002/1‑70 (MQ=255)
      gTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaat                                                               >  1:691215/1‑70 (MQ=255)
       tCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaatc                                                              >  1:3135680/1‑70 (MQ=255)
           ccTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATcaatcaat                                                           <  1:1824903/69‑1 (MQ=255)
           ccTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATcaatcaat                                                           <  1:2005816/69‑1 (MQ=255)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatca                                                                 >  1:909311/1‑62 (MQ=255)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAAttt                                                         <  1:2127895/70‑1 (MQ=255)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAAttt                                                         >  1:3390287/1‑70 (MQ=255)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTg                                                        <  1:565818/71‑1 (MQ=255)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTGATTGATAAATAAAttt                                                         <  1:1425912/70‑1 (MQ=38)
            cTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTGATTGATAAATAAAttt                                                         <  1:1306927/70‑1 (MQ=38)
               cccGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGAc                                                      <  1:1445837/70‑1 (MQ=255)
                ccGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACtt                                                    <  1:367114/71‑1 (MQ=255)
                  gTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAAttt                                                         <  1:2538563/64‑1 (MQ=255)
                   tAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGTCTTAag                                                 <  1:2191790/71‑1 (MQ=255)
                   tAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACtt                                                    >  1:3290008/1‑68 (MQ=255)
                   tAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAag                                                 <  1:2890386/71‑1 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAAtt                                                          >  1:1098002/1‑58 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAggg                                             <  1:3294031/71‑1 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAggg                                             <  1:3197294/71‑1 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAggg                                             >  1:904483/1‑71 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAggg                                             >  1:1044403/1‑71 (MQ=255)
                       aCATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAgg                                              >  1:2060597/1‑70 (MQ=255)
                         aTCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATATATCAATCAATTTGACTTAAGAGggcg                                           <  1:272094/71‑1 (MQ=255)
                            acacaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGgcggcg                                        <  1:178817/71‑1 (MQ=255)
                            acacaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGgcggcg                                        >  1:3411042/1‑71 (MQ=255)
                             cacaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGt                                       >  1:580942/1‑71 (MQ=255)
                              acaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaa                                                                >  1:3397778/1‑45 (MQ=255)
                              acaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTatcaa                                                                >  1:10237/1‑45 (MQ=255)
                              acaTTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTg                                      >  1:1666089/1‑71 (MQ=255)
                                 ttATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:19377/56‑1 (MQ=255)
                                 ttATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:1974741/56‑1 (MQ=255)
                                 ttATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTa                                   <  1:1851042/71‑1 (MQ=255)
                                 ttATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTa                                   >  1:1086867/1‑71 (MQ=255)
                                 ttATCATCCTGTTCTCCCCCTTCGTTTAAATTTATTTATCAGTCAATTTGACTTAAGAGGGCGGCGt                                       <  1:3165491/67‑1 (MQ=255)
                                  tATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTa                                                   >  1:2690292/1‑54 (MQ=255)
                                   atcatcCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAaga                                                >  1:1798870/1‑56 (MQ=255)
                                   atcatcCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGc                                     >  1:2153784/1‑67 (MQ=255)
                                          tGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATtaata                           >  1:2970776/1‑70 (MQ=255)
                                           gTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:1059924/46‑1 (MQ=255)
                                            ttCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACtt                                                    <  1:1021886/43‑1 (MQ=255)
                                            ttCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACtt                                                    <  1:2667430/43‑1 (MQ=255)
                                            ttCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATtaataa                          <  1:3655348/69‑1 (MQ=255)
                                             tctcCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  >  1:3555259/1‑44 (MQ=255)
                                             tctcCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  >  1:771254/1‑44 (MQ=255)
                                                cccGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTa                                                   >  1:2952734/1‑40 (MQ=255)
                                                cccGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTa                                                   >  1:1140470/1‑40 (MQ=255)
                                                 ccGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGt                     <  1:1554057/69‑1 (MQ=255)
                                                 ccGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTaa                   >  1:3233771/1‑71 (MQ=255)
                                                  cGCTTCGTTTAAATTTATTTATCAATCAATTTGAc                                                      <  1:831075/35‑1 (MQ=255)
                                                   gCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTa                    <  1:2293343/68‑1 (MQ=255)
                                                   gCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAata                 >  1:1848056/1‑71 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:3255857/37‑1 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:1804412/37‑1 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:276182/37‑1 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:2467362/37‑1 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAatat                <  1:3225349/71‑1 (MQ=255)
                                                    cTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAatat                <  1:2079968/71‑1 (MQ=255)
                                                    cTTCGTTAAAATTTATTTATCAATCAATTTGACTTaa                                                  <  1:1204978/37‑1 (MQ=255)
                                                      tCGTTTAAATTTATTTATCAATCAATTTGACTTAaga                                                >  1:2005608/1‑37 (MQ=255)
                                                      tCGTTTAAATTTATTTATCAATCAATTTGACTTAaga                                                >  1:982889/1‑37 (MQ=255)
                                                      tCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGt              >  1:1535474/1‑71 (MQ=255)
                                                       cGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACa                                 >  1:3379044/1‑51 (MQ=255)
                                                       cGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACa                                 >  1:146752/1‑51 (MQ=255)
                                                        gTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGttt            <  1:3117727/71‑1 (MQ=255)
                                                         tttAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATtaa                             <  1:969475/53‑1 (MQ=255)
                                                           tAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATtaata                           >  1:3535823/1‑53 (MQ=255)
                                                           tAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATg               >  1:2097384/1‑65 (MQ=255)
                                                           tAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATg         >  1:2567956/1‑71 (MQ=255)
                                                           tAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATg         >  1:2548744/1‑71 (MQ=255)
                                                             aaTTGATTGATAAATAAATTTGACTTAAGAGGGCGGCGTGCTACa                                 >  1:1288572/1‑45 (MQ=18)
                                                             aaTTGATTGATAAATAAATTTGACTTAAGAGGGCGGCGTGCTACa                                 >  1:2798134/1‑45 (MQ=18)
                                                              atttatttATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATaaa                         <  1:622334/52‑1 (MQ=255)
                                                                 tatttatCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAatat   >  1:122147/1‑71 (MQ=255)
                                                                 tatttatCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAata    >  1:3379099/1‑70 (MQ=255)
                                                                   tttatCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAATAtt  >  1:817241/1‑70 (MQ=255)
                                                                     tatCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAATAtt  <  1:3512723/68‑1 (MQ=255)
                                                                     |                                                                   
AATCCAGTCATCCTGCCCGTAGAACATCACACATTATCATCCTGTTCTCCCGCTTCGTTTAAATTTATTTATCAATCAATTTGACTTAAGAGGGCGGCGTGCTACATTAATAAACAGTAATATGTTTATGTAATATT  >  Exported/2581734‑2581870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: