Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,585,230 G→A 7.8% L683L (CTG→TTG)  mdtF ← multidrug transporter, RpoS‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,585,2300GA 7.8% 240.3 / 8.3 77L683L (CTG→TTG) mdtFmultidrug transporter, RpoS‑dependent
Reads supporting (aligned to +/- strand):  ref base G (37/34);  new base A (4/2);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAGACGACG  >  Exported/2585162‑2585300
                                                                    |                                                                      
cgcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca                                                                      <  1:1138350/71‑1 (MQ=255)
cgcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca                                                                      <  1:721880/71‑1 (MQ=255)
  cTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGt                                                                    <  1:1647837/71‑1 (MQ=255)
     ccAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca                                                                      <  1:1785704/66‑1 (MQ=255)
     ccAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca                                                                      <  1:694845/66‑1 (MQ=255)
       aGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa                                                                >  1:356715/1‑70 (MQ=255)
        gTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCata                                                              <  1:2082115/71‑1 (MQ=255)
        gTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCata                                                              <  1:1187291/71‑1 (MQ=255)
              aCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaaa                                                         <  1:2737241/70‑1 (MQ=255)
              aCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaaa                                                         <  1:562683/70‑1 (MQ=255)
                 gCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt                                                     <  1:1970361/71‑1 (MQ=255)
                      tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCatat                                                             <  1:1496817/58‑1 (MQ=255)
                      tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc                                                >  1:98422/1‑71 (MQ=255)
                      tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc                                                <  1:1369589/71‑1 (MQ=255)
                       ttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg                                               >  1:881507/1‑71 (MQ=255)
                        tCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc                                                >  1:3408405/1‑69 (MQ=255)
                        tCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc                                                >  1:3352281/1‑69 (MQ=255)
                          gcgcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGt                                             <  1:1196356/70‑1 (MQ=255)
                          gcgcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTa                                            >  1:2893587/1‑71 (MQ=255)
                            gcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc                                          >  1:1614328/1‑71 (MQ=255)
                                 ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg                                               <  1:2081913/61‑1 (MQ=255)
                                 ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg                                               <  1:881315/61‑1 (MQ=255)
                                 ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGtt                                     <  1:708476/71‑1 (MQ=255)
                                 ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGt                                      <  1:3382272/70‑1 (MQ=255)
                                     aGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc                                 >  1:1290637/1‑71 (MQ=255)
                                     aGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc                                 >  1:678412/1‑71 (MQ=255)
                                      gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGcc                                <  1:3535982/71‑1 (MQ=255)
                                      gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc                                 <  1:3313541/70‑1 (MQ=255)
                                      gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc                                 <  1:3028981/70‑1 (MQ=255)
                                       tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTc                                                                  <  1:633324/36‑1 (MQ=255)
                                       tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGcc                                <  1:984119/70‑1 (MQ=255)
                                         tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc                             >  1:397069/1‑71 (MQ=255)
                                         tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc                             >  1:3449289/1‑71 (MQ=255)
                                         tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc                              >  1:3618945/1‑70 (MQ=255)
                                         tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc                              >  1:3590049/1‑70 (MQ=255)
                                         tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc                              >  1:1576110/1‑70 (MQ=255)
                                           tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt                                                     <  1:2022630/45‑1 (MQ=255)
                                           tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt                                                     <  1:861118/45‑1 (MQ=255)
                                           tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt                                                     <  1:3060623/45‑1 (MQ=255)
                                            cGTGCCCCAGGTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACcgc                           <  1:300197/70‑1 (MQ=255)
                                               gCCCCAGGTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc                                 >  1:3510958/1‑61 (MQ=255)
                                                 cccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa                      <  1:653203/70‑1 (MQ=255)
                                                 cccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa                      <  1:3254840/70‑1 (MQ=255)
                                                  ccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg                    <  1:3595215/71‑1 (MQ=255)
                                                  ccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg                    <  1:1491265/71‑1 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc                                          >  1:1206461/1‑48 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc                                          >  1:1739085/1‑48 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAc                                           >  1:3354240/1‑47 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg                    >  1:477904/1‑70 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg                    >  1:3060016/1‑70 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt                   <  1:1842426/71‑1 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt                   >  1:620284/1‑71 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt                   >  1:2481506/1‑71 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt                   <  1:2089377/71‑1 (MQ=255)
                                                   cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt                   <  1:1445768/71‑1 (MQ=255)
                                                    aGGTTACCGTTGTGCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  <  1:2837994/71‑1 (MQ=255)
                                                     ggTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  >  1:2874993/1‑70 (MQ=255)
                                                     ggTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  >  1:2439135/1‑70 (MQ=255)
                                                      gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg                                               >  1:2461048/1‑40 (MQ=255)
                                                      gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTGAGCCACCGCGGGTaa                     >  1:3428562/1‑66 (MQ=255)
                                                      gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTaa                     >  1:1308015/1‑66 (MQ=255)
                                                      gTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  >  1:67669/1‑69 (MQ=255)
                                                       ttACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGaa               <  1:1691962/71‑1 (MQ=255)
                                                        tACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  >  1:3698138/1‑67 (MQ=255)
                                                        tACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt                  >  1:644372/1‑67 (MQ=255)
                                                         aCCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg             >  1:67386/1‑71 (MQ=255)
                                                         aCCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCggg                        >  1:2933130/1‑60 (MQ=255)
                                                          ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg            >  1:1734056/1‑71 (MQ=255)
                                                          ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg            >  1:163357/1‑71 (MQ=255)
                                                          ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg             <  1:698443/70‑1 (MQ=255)
                                                          ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg             <  1:2992277/70‑1 (MQ=255)
                                                           cGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc                             <  1:2247219/53‑1 (MQ=255)
                                                           cGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc                             <  1:1170801/53‑1 (MQ=255)
                                                            gTTGTCCAGCAGTTCCATATCAAGACCTGACGCGGTACCCAGTTc                                    <  1:1757567/45‑1 (MQ=255)
                                                             ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg           >  1:3463748/1‑69 (MQ=255)
                                                             ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg           >  1:3429972/1‑69 (MQ=255)
                                                             ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg           >  1:1286245/1‑69 (MQ=255)
                                                             ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg            >  1:346118/1‑68 (MQ=255)
                                                             ttGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg           >  1:1589829/1‑69 (MQ=255)
                                                                tCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTaa                     >  1:3561571/1‑56 (MQ=255)
                                                                tCCAGCAGTTCCATATCAAAACCGGACGCGGTACCCAGTTCAGCCACCGCGGGTaa                     >  1:3419662/1‑56 (MQ=255)
                                                                   agcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa                      <  1:2629559/52‑1 (MQ=255)
                                                                   agcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAgacgac   <  1:3411808/71‑1 (MQ=255)
                                                                    gcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCa                               >  1:282974/1‑42 (MQ=255)
                                                                    gcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAACacgacg  >  1:1613101/1‑71 (MQ=255)
                                                                    |                                                                      
CGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAGACGACG  >  Exported/2585162‑2585300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: