| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,585,230 | G→A | 7.8% | L683L (CTG→TTG) | mdtF ← | multidrug transporter, RpoS‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,585,230 | 0 | G | A | 7.8% | 240.3 / 8.3 | 77 | L683L (CTG→TTG) | mdtF | multidrug transporter, RpoS‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (37/34); new base A (4/2); total (41/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.79e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAGACGACG > Exported/2585162‑2585300 | cgcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca < 1:1138350/71‑1 (MQ=255)cgcTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca < 1:721880/71‑1 (MQ=255) cTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGt < 1:1647837/71‑1 (MQ=255) ccAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca < 1:1785704/66‑1 (MQ=255) ccAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCcagca < 1:694845/66‑1 (MQ=255) aGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCa > 1:356715/1‑70 (MQ=255) gTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCata < 1:2082115/71‑1 (MQ=255) gTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCata < 1:1187291/71‑1 (MQ=255) aCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaaa < 1:2737241/70‑1 (MQ=255) aCAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCaaa < 1:562683/70‑1 (MQ=255) gCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt < 1:1970361/71‑1 (MQ=255) tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCatat < 1:1496817/58‑1 (MQ=255) tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc > 1:98422/1‑71 (MQ=255) tttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc < 1:1369589/71‑1 (MQ=255) ttCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg > 1:881507/1‑71 (MQ=255) tCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc > 1:3408405/1‑69 (MQ=255) tCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgc > 1:3352281/1‑69 (MQ=255) gcgcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGt < 1:1196356/70‑1 (MQ=255) gcgcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTa > 1:2893587/1‑71 (MQ=255) gcCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc > 1:1614328/1‑71 (MQ=255) ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg < 1:2081913/61‑1 (MQ=255) ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg < 1:881315/61‑1 (MQ=255) ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGtt < 1:708476/71‑1 (MQ=255) ggTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGt < 1:3382272/70‑1 (MQ=255) aGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc > 1:1290637/1‑71 (MQ=255) aGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc > 1:678412/1‑71 (MQ=255) gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGcc < 1:3535982/71‑1 (MQ=255) gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc < 1:3313541/70‑1 (MQ=255) gTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc < 1:3028981/70‑1 (MQ=255) tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTc < 1:633324/36‑1 (MQ=255) tttttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGcc < 1:984119/70‑1 (MQ=255) tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc > 1:397069/1‑71 (MQ=255) tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc > 1:3449289/1‑71 (MQ=255) tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc > 1:3618945/1‑70 (MQ=255) tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc > 1:3590049/1‑70 (MQ=255) tttCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAc > 1:1576110/1‑70 (MQ=255) tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt < 1:2022630/45‑1 (MQ=255) tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt < 1:861118/45‑1 (MQ=255) tCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCt < 1:3060623/45‑1 (MQ=255) cGTGCCCCAGGTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACcgc < 1:300197/70‑1 (MQ=255) gCCCCAGGTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGc > 1:3510958/1‑61 (MQ=255) cccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa < 1:653203/70‑1 (MQ=255) cccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa < 1:3254840/70‑1 (MQ=255) ccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg < 1:3595215/71‑1 (MQ=255) ccAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg < 1:1491265/71‑1 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc > 1:1206461/1‑48 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAcc > 1:1739085/1‑48 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTAc > 1:3354240/1‑47 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg > 1:477904/1‑70 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAg > 1:3060016/1‑70 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt < 1:1842426/71‑1 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt > 1:620284/1‑71 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt > 1:2481506/1‑71 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt < 1:2089377/71‑1 (MQ=255) cAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGt < 1:1445768/71‑1 (MQ=255) aGGTTACCGTTGTGCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt < 1:2837994/71‑1 (MQ=255) ggTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt > 1:2874993/1‑70 (MQ=255) ggTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt > 1:2439135/1‑70 (MQ=255) gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGAcgcg > 1:2461048/1‑40 (MQ=255) gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTGAGCCACCGCGGGTaa > 1:3428562/1‑66 (MQ=255) gTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTaa > 1:1308015/1‑66 (MQ=255) gTTACCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt > 1:67669/1‑69 (MQ=255) ttACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGaa < 1:1691962/71‑1 (MQ=255) tACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt > 1:3698138/1‑67 (MQ=255) tACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGtt > 1:644372/1‑67 (MQ=255) aCCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg > 1:67386/1‑71 (MQ=255) aCCGTTGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCggg > 1:2933130/1‑60 (MQ=255) ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg > 1:1734056/1‑71 (MQ=255) ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg > 1:163357/1‑71 (MQ=255) ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg < 1:698443/70‑1 (MQ=255) ccGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACg < 1:2992277/70‑1 (MQ=255) cGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc < 1:2247219/53‑1 (MQ=255) cGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCAcc < 1:1170801/53‑1 (MQ=255) gTTGTCCAGCAGTTCCATATCAAGACCTGACGCGGTACCCAGTTc < 1:1757567/45‑1 (MQ=255) ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg > 1:3463748/1‑69 (MQ=255) ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg > 1:3429972/1‑69 (MQ=255) ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg > 1:1286245/1‑69 (MQ=255) ttGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACgg > 1:346118/1‑68 (MQ=255) ttGTCCAACAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACggg > 1:1589829/1‑69 (MQ=255) tCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTaa > 1:3561571/1‑56 (MQ=255) tCCAGCAGTTCCATATCAAAACCGGACGCGGTACCCAGTTCAGCCACCGCGGGTaa > 1:3419662/1‑56 (MQ=255) agcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTa < 1:2629559/52‑1 (MQ=255) agcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAgacgac < 1:3411808/71‑1 (MQ=255) gcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCa > 1:282974/1‑42 (MQ=255) gcagTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAACacgacg > 1:1613101/1‑71 (MQ=255) | CGCTGCCAGTGATAACAGCTCGTTTCGCGCCTGGGTTAGTTTTTCGTGCCCCAGGTTACCGTTGTCCAGCAGTTCCATATCAAAACCTGACGCGGTACCCAGTTCAGCCACCGCGGGTAAGTTGAACGGGAAGACGACG > Exported/2585162‑2585300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |