| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,589,325 | A→G | 5.5% | intergenic (‑1/+798) | gadE ← / ← hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,589,325 | 0 | A | G | 5.5% | 271.8 / 5.4 | 91 | intergenic (‑1/+798) | gadE/hdeD | DNA‑binding transcriptional activator/acid‑resistance membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (43/43); new base G (3/2); total (46/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCG > Exported/2589258‑2589394 | cGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATGAc < 1:277908/70‑1 (MQ=255) gTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACt < 1:1202983/70‑1 (MQ=255) ttATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTc < 1:2947244/71‑1 (MQ=255) tCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATa < 1:3316066/60‑1 (MQ=255) tCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt < 1:1007324/70‑1 (MQ=255) tCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCt < 1:2954330/70‑1 (MQ=255) ttCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAg > 1:770270/1‑70 (MQ=255) aCTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:411385/71‑1 (MQ=255) aCTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGtt < 1:872636/71‑1 (MQ=255) tGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTAt > 1:152948/1‑71 (MQ=255) gCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATc > 1:3421673/1‑71 (MQ=255) gCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATc > 1:294022/1‑71 (MQ=255) ccAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCg > 1:3136213/1‑71 (MQ=255) aaaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGtt < 1:3097158/71‑1 (MQ=255) aaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGttt > 1:3404108/1‑71 (MQ=255) aaaGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGttt > 1:3031941/1‑71 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCg < 1:1025825/71‑1 (MQ=255) aGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCg < 1:2913354/71‑1 (MQ=255) aGCCCAGTAAAAGAAAAGAATCTTTCGACATGAGAAAAATAATAACTTg > 1:260348/1‑49 (MQ=255) cTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg > 1:1513661/1‑56 (MQ=255) cTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCg > 1:1620270/1‑56 (MQ=255) gTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTc < 1:1227191/64‑1 (MQ=255) gTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATg > 1:3329020/1‑71 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACtt > 1:2220901/1‑41 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATg > 1:2707818/1‑70 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATg < 1:463475/70‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATg < 1:2167714/70‑1 (MQ=255) tAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGt > 1:3080267/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:1747397/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:2732091/1‑71 (MQ=255) aaagaaaagaATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCa > 1:2703767/1‑71 (MQ=255) aagaaaagaaTCTTTCGTCATGAGAAAAATCATaa < 1:472428/35‑1 (MQ=255) aagaaaagaaTCTTTCGTCATGAGAAAAATCATaa < 1:3033763/35‑1 (MQ=255) aagaaaagaaTCCTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGt < 1:2530846/57‑1 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTa > 1:239084/1‑50 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTa > 1:1543182/1‑50 (MQ=255) aaaagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCt < 1:347416/71‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGc < 1:3344520/35‑1 (MQ=255) aagaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATc < 1:3732571/67‑1 (MQ=255) agaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCtt < 1:1017395/39‑1 (MQ=255) agaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCtt < 1:1715702/39‑1 (MQ=255) agaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCtt < 1:3572584/39‑1 (MQ=255) agaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCtt < 1:533013/39‑1 (MQ=255) gaaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTa > 1:1626248/1‑46 (MQ=255) aaTCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGa < 1:911959/54‑1 (MQ=255) tttCGTCATGAGAAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCat > 1:3076725/1‑68 (MQ=255) ttCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt > 1:887190/1‑71 (MQ=255) tCGTCATGAGAAAAATGATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCatta < 1:3170674/68‑1 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAatat > 1:3549818/1‑52 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt > 1:2440129/1‑70 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt > 1:2958329/1‑70 (MQ=255) tCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattat < 1:1436929/69‑1 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCattatt < 1:2105526/65‑1 (MQ=255) aTGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATc > 1:2259536/1‑71 (MQ=255) gagaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTa > 1:1137151/1‑65 (MQ=255) gagaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCt > 1:1015217/1‑71 (MQ=255) agaAAAATCATGACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:3656981/41‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:1808071/41‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:3221243/41‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACa > 1:1195560/1‑66 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCtt > 1:71580/1‑71 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCtt < 1:3701848/71‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCtt < 1:310357/71‑1 (MQ=255) agaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCtt < 1:1253360/71‑1 (MQ=255) gaAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCtt > 1:339370/1‑70 (MQ=255) aaaaaTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:2892401/1‑41 (MQ=255) aaaaaTCATGACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1582045/1‑41 (MQ=255) aaaaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:2673091/1‑41 (MQ=255) aaaaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:3495375/1‑41 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTc > 1:2261837/1‑43 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATcc > 1:2712451/1‑47 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc > 1:1690930/1‑52 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTc > 1:3548757/1‑52 (MQ=255) aaaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTcc > 1:1035827/1‑71 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:2875124/36‑1 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:1225043/36‑1 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGaa < 1:417188/36‑1 (MQ=255) aaTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg < 1:1868650/71‑1 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGt > 1:533993/1‑67 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg > 1:445199/1‑70 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg > 1:982443/1‑70 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCg > 1:1286811/1‑70 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGa < 1:3565501/71‑1 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGa < 1:3079567/71‑1 (MQ=255) aTCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGa < 1:850601/71‑1 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAata > 1:1025503/1‑36 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCtt < 1:2790955/46‑1 (MQ=255) tCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGaa < 1:1239227/71‑1 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAAt < 1:1009950/70‑1 (MQ=255) aTAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATc > 1:3322158/1‑71 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGt < 1:117363/36‑1 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAAt < 1:323192/69‑1 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCg < 1:2359310/71‑1 (MQ=255) tAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCg < 1:1901134/71‑1 (MQ=255) | CGTGATTATCTTTCAACTGCCAAAAGCCCTGTAAAAGAAAAGAATCTTTCGTCATGAGAAAAATCATAACTTGCTCCTTAGCCGTTATCGTTTCGAATATGTCATCCTTTTCATTATTTACATCCTTGTCCGAATCG > Exported/2589258‑2589394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |