Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,597,519 T→A 9.2% intergenic (‑515/+362) arsR ← / ← gor DNA‑binding transcriptional regulator/glutathione oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,597,5190TA 9.2% 339.4 / 19.9 118intergenic (‑515/+362)arsR/gorDNA‑binding transcriptional regulator/glutathione oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (56/51);  new base A (6/5);  total (62/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 122 total aligned reads displayed.

GTGAGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATAAA  >  Exported/2597450‑2597589
                                                                     |                                                                      
gTGAGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGttt                                                                        >  1:504522/1‑70 (MQ=255)
 tGAGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTta                                                                      >  1:1323260/1‑71 (MQ=255)
 tGAGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTta                                                                      >  1:3056062/1‑71 (MQ=255)
  gagaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTta                                                                      >  1:3236343/1‑70 (MQ=255)
   agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtata                                                                    <  1:343531/71‑1 (MQ=255)
   agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtata                                                                    <  1:1454483/71‑1 (MQ=255)
   agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2585008/70‑1 (MQ=255)
   agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2764185/70‑1 (MQ=255)
   agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2866896/70‑1 (MQ=255)
     aTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAc                                                                  >  1:598080/1‑71 (MQ=255)
     aTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAc                                                                  >  1:3231164/1‑71 (MQ=255)
      tAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACCTGCTGGTTTTATaa                                                                   <  1:3480275/69‑1 (MQ=255)
       aGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtata                                                                    <  1:2711571/67‑1 (MQ=255)
       aGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                >  1:400622/1‑71 (MQ=255)
        gCCATCATCTAACGCATCAATGCTTATACCATTCTGAAACTATTGCAAGGACGTGCTGGTTTTATAAcc                                                                 >  1:370953/1‑69 (MQ=255)
        gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAcc                                                                 >  1:232454/1‑69 (MQ=255)
        gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAcc                                                                 >  1:1288446/1‑69 (MQ=255)
        gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:271625/70‑1 (MQ=255)
            tcatcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATg                                                           <  1:3347005/71‑1 (MQ=255)
            tcatcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATg                                                           >  1:2247123/1‑71 (MQ=255)
             catcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     >  1:1344545/1‑60 (MQ=255)
              atcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTa                                                         <  1:1046118/71‑1 (MQ=255)
                cTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCAt                                                            >  1:2860795/1‑66 (MQ=255)
                 tAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTg                                                      >  1:3069029/1‑71 (MQ=255)
                   aCGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTg                                                      <  1:1412522/69‑1 (MQ=255)
                         cAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              >  1:2179839/1‑71 (MQ=255)
                         cAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              >  1:3053106/1‑71 (MQ=255)
                          aaTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              <  1:3498069/70‑1 (MQ=255)
                          aaTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              <  1:1106185/70‑1 (MQ=255)
                           aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:1743896/51‑1 (MQ=255)
                           aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:234458/51‑1 (MQ=255)
                           aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:3193301/51‑1 (MQ=255)
                           aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATc                                            <  1:1094330/71‑1 (MQ=255)
                            tGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCa                                                             >  1:74172/1‑53 (MQ=255)
                            tGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCa                                                             >  1:3453262/1‑53 (MQ=255)
                            tGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTAt                                             >  1:204211/1‑69 (MQ=255)
                               tAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTAc                                                        >  1:1300914/1‑55 (MQ=255)
                                aaTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:553935/1‑70 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGa                                                 >  1:1554702/1‑60 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGa                                                 >  1:1665568/1‑60 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAg                                         >  1:774673/1‑68 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGtt                                       <  1:3347621/70‑1 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:3196750/1‑69 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:3600606/1‑69 (MQ=255)
                                 aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTa                                      <  1:791380/71‑1 (MQ=255)
                                   aCCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGt                                                     >  1:2021301/1‑54 (MQ=255)
                                   aCCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGt                                                     >  1:3063566/1‑54 (MQ=255)
                                     cATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctc                                  <  1:641844/71‑1 (MQ=255)
                                      aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:2596128/71‑1 (MQ=255)
                                      aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:808780/71‑1 (MQ=255)
                                      aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:1748427/71‑1 (MQ=255)
                                      aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:1797642/1‑71 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATc                                            >  1:262560/1‑59 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:1699891/1‑70 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:611462/1‑70 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:110745/1‑70 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:3515820/1‑70 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctc                                  >  1:827149/1‑69 (MQ=255)
                                       tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:1896531/71‑1 (MQ=255)
                                        aCTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGc                                                              <  1:2509656/40‑1 (MQ=255)
                                        aCTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGc                                                              <  1:615820/40‑1 (MQ=255)
                                         cTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGc                                                              <  1:2748524/39‑1 (MQ=255)
                                         cTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACt                                                       <  1:2831176/46‑1 (MQ=255)
                                          tGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCAt                                                            >  1:3371760/1‑40 (MQ=255)
                                           gAAACTATTGCAAGGACGTGCTGGTTTTATAACCTg                                                               >  1:1863051/1‑36 (MQ=255)
                                           gAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAgg                             >  1:106203/1‑70 (MQ=255)
                                            aaaCTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGc                            <  1:3189504/70‑1 (MQ=255)
                                                tATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  >  1:2565124/1‑44 (MQ=255)
                                                  ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  <  1:2406202/42‑1 (MQ=255)
                                                  ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  <  1:2401840/42‑1 (MQ=255)
                                                  ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCAtttt                       <  1:3195880/69‑1 (MQ=255)
                                                  ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCAtttt                       <  1:801549/69‑1 (MQ=255)
                                                   tGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGa                                                 <  1:873837/42‑1 (MQ=255)
                                                    gCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:706716/57‑1 (MQ=255)
                                                    gCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCAct                   >  1:3529837/1‑71 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:2181717/56‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:867750/56‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:2789489/71‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1275403/71‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1356944/71‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:298291/71‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1662676/71‑1 (MQ=255)
                                                      aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1715039/71‑1 (MQ=255)
                                                       aGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTg                >  1:719788/1‑71 (MQ=255)
                                                        ggACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGc                            >  1:2143394/1‑58 (MQ=255)
                                                          aCGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCa             <  1:974962/71‑1 (MQ=255)
                                                             tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:1837684/49‑1 (MQ=25)
                                                             tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:754523/49‑1 (MQ=25)
                                                             tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:422361/49‑1 (MQ=25)
                                                              gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgc         >  1:3559915/4‑71 (MQ=255)
                                                              gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgc         >  1:1773080/4‑71 (MQ=255)
                                                              gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTAACTCTGGCAATgc         >  1:3026147/4‑71 (MQ=255)
                                                               caggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAg                              >  1:86314/3‑49 (MQ=25)
                                                                tGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgcg        >  1:1667402/1‑70 (MQ=255)
                                                                tGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgcg        >  1:3448464/1‑70 (MQ=255)
                                                                 ggTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCa      >  1:190786/1‑71 (MQ=255)
                                                                 ggTTATAAAACCAGCACGTACTGTATGATTATCCAGTTAGctct                                 >  1:1486738/1‑44 (MQ=21)
                                                                  gTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCAt     <  1:1989709/71‑1 (MQ=255)
                                                                     ttATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATaaa  >  1:2026377/1‑71 (MQ=255)
                                                                     ttATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATaa   <  1:2193967/70‑1 (MQ=255)
                                                                     |                                                                      
GTGAGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATAAA  >  Exported/2597450‑2597589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: