Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,597,522 T→A 10.0% intergenic (‑518/+359) arsR ← / ← gor DNA‑binding transcriptional regulator/glutathione oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,597,5220TA10.0% 312.8 / 22.3 110intergenic (‑518/+359)arsR/gorDNA‑binding transcriptional regulator/glutathione oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (53/46);  new base A (6/5);  total (59/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

Only 100 of 119 total aligned reads displayed.

AGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATAAA  >  Exported/2597453‑2597589
                                                                     |                                                                   
agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtata                                                                    <  1:343531/71‑1 (MQ=255)
agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtata                                                                    <  1:1454483/71‑1 (MQ=255)
agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2866896/70‑1 (MQ=255)
agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2764185/70‑1 (MQ=255)
agaTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     <  1:2585008/70‑1 (MQ=255)
  aTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAc                                                                  >  1:3231164/1‑71 (MQ=255)
  aTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAc                                                                  >  1:1925103/1‑71 (MQ=255)
  aTAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAc                                                                  >  1:598080/1‑71 (MQ=255)
   tAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACCTGCTGGTTTTATaa                                                                   <  1:3480275/69‑1 (MQ=255)
    aGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                >  1:400622/1‑71 (MQ=255)
     gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAcc                                                                 >  1:1288446/1‑69 (MQ=255)
     gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAAcc                                                                 >  1:232454/1‑69 (MQ=255)
     gCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:271625/70‑1 (MQ=255)
         tcatcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATg                                                           <  1:3347005/71‑1 (MQ=255)
         tcatcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATg                                                           <  1:1706700/71‑1 (MQ=255)
         tcatcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATg                                                           >  1:2247123/1‑71 (MQ=255)
          catcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTtat                                                                     >  1:1344545/1‑60 (MQ=255)
           atcTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTa                                                         <  1:1046118/71‑1 (MQ=255)
             cTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCAt                                                            >  1:2860795/1‑66 (MQ=255)
              tAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTg                                                      >  1:3069029/1‑71 (MQ=255)
                 cGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTAt                                                   >  1:3603340/1‑71 (MQ=255)
                 cGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTAt                                                   <  1:2283179/71‑1 (MQ=255)
                 cGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTAt                                                   >  1:1354968/1‑71 (MQ=255)
                      cAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              >  1:2179839/1‑71 (MQ=255)
                       aaTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTa                                              <  1:1106185/70‑1 (MQ=255)
                        aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:1743896/51‑1 (MQ=255)
                        aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:234458/51‑1 (MQ=255)
                        aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:3193301/51‑1 (MQ=255)
                        aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCt                                                                <  1:832957/51‑1 (MQ=255)
                        aTGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATc                                            <  1:1094330/71‑1 (MQ=255)
                         tGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCa                                                             >  1:74172/1‑53 (MQ=255)
                         tGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCa                                                             >  1:3453262/1‑53 (MQ=255)
                            tAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTAc                                                        >  1:1300914/1‑55 (MQ=255)
                            tAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:1211607/1‑71 (MQ=255)
                             aaTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:553935/1‑70 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGa                                                 >  1:1554702/1‑60 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAg                                         >  1:774673/1‑68 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGtt                                       <  1:3347621/70‑1 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:3223519/1‑69 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGt                                        >  1:3600606/1‑69 (MQ=255)
                              aTACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTa                                      <  1:791380/71‑1 (MQ=255)
                               tACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAg                                     <  1:3017796/71‑1 (MQ=255)
                                aCCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGt                                                     >  1:3063566/1‑54 (MQ=255)
                                aCCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGt                                                     >  1:2021301/1‑54 (MQ=255)
                                  cATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctc                                  <  1:641844/71‑1 (MQ=255)
                                   aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:516266/1‑71 (MQ=255)
                                   aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:2596128/71‑1 (MQ=255)
                                   aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:808780/71‑1 (MQ=255)
                                   aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:1748427/71‑1 (MQ=255)
                                   aTACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:1797642/1‑71 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTAt                                             >  1:3480440/1‑58 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATc                                            >  1:262560/1‑59 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:611462/1‑70 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:1699891/1‑70 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:3515820/1‑70 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 >  1:110745/1‑70 (MQ=255)
                                    tACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:1896531/71‑1 (MQ=255)
                                     aCTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGc                                                              <  1:2509656/40‑1 (MQ=255)
                                      cTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGc                                                              <  1:2748524/39‑1 (MQ=255)
                                      cTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACt                                                       <  1:762892/46‑1 (MQ=255)
                                      cTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGct                                   >  1:3183120/1‑66 (MQ=255)
                                       tGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCAt                                                            >  1:2080459/1‑40 (MQ=255)
                                       tGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCAt                                                            >  1:3371760/1‑40 (MQ=255)
                                        gAAACTATTGCAAGGACGTGCTGGTTTTATAACCTg                                                               >  1:1863051/1‑36 (MQ=255)
                                        gAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAgg                             >  1:106203/1‑70 (MQ=255)
                                         aaaCTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGc                            <  1:3189504/70‑1 (MQ=255)
                                             tATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  >  1:2565124/1‑44 (MQ=255)
                                               ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  <  1:2401840/42‑1 (MQ=255)
                                               ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATg                                                  <  1:2406202/42‑1 (MQ=255)
                                               ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCAtttt                       <  1:3195880/69‑1 (MQ=255)
                                               ttGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCAtttt                       <  1:801549/69‑1 (MQ=255)
                                                tGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGa                                                 <  1:873837/42‑1 (MQ=255)
                                                 gCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGctct                                 <  1:706716/57‑1 (MQ=255)
                                                 gCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCAct                   >  1:3529837/1‑71 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:867750/56‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:2181717/56‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1356944/71‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:2789489/71‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1662676/71‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:1715039/71‑1 (MQ=255)
                                                   aaGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCActct                 <  1:298291/71‑1 (MQ=255)
                                                    aGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTg                >  1:719788/1‑71 (MQ=255)
                                                    aGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTg                >  1:697735/1‑71 (MQ=255)
                                                     ggACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGc                            >  1:2143394/1‑58 (MQ=255)
                                                       aCGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCa             <  1:974962/71‑1 (MQ=255)
                                                          tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:1837684/49‑1 (MQ=25)
                                                          tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:422361/49‑1 (MQ=25)
                                                          tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:1528762/49‑1 (MQ=25)
                                                          tGCAGGTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTg                                <  1:278000/49‑1 (MQ=25)
                                                           gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgc         >  1:179605/4‑71 (MQ=255)
                                                           gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgc         >  1:3559915/4‑71 (MQ=255)
                                                           gcaggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTAACTCTGGCAATgc         >  1:3026147/4‑71 (MQ=255)
                                                            caggTTATAAAACCAGCATGTACTGTATGATTATCCAGTTAGCTCTGAg                              >  1:86314/3‑49 (MQ=25)
                                                             tGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgcg        >  1:1667402/1‑70 (MQ=255)
                                                             tGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATgcg        >  1:3448464/1‑70 (MQ=255)
                                                              ggTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCa      >  1:190786/1‑71 (MQ=255)
                                                              ggTTATAAAACCAGCACGTACTGTATGATTATCCAGTTAGctct                                 >  1:1486738/1‑44 (MQ=21)
                                                               gTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCAt     <  1:1989709/71‑1 (MQ=255)
                                                                  ttATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATaaa  >  1:2026377/1‑71 (MQ=255)
                                                                  ttATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATaa   <  1:2193967/70‑1 (MQ=255)
                                                                     |                                                                   
AGATAGCCATCATCTAACGCATCAATGCTAATACCATACTGAAACTATTGCAAGGACGTGCTGGTTTTATAACCTGCATGTACTGTATGATTATCCAGTTAGCTCTGAGGCATTTTCACTCTGGCAATGCGCATAAA  >  Exported/2597453‑2597589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: