| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,613,354 | C→T | 9.2% | E57K (GAA→AAA) | nikA ← | nickel transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,613,354 | 0 | C | T | 9.2% | 291.5 / 15.6 | 99 | E57K (GAA→AAA) | nikA | nickel transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (46/41); new base T (5/4); total (52/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
AATGAGTCCAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACATTCAC > Exported/2613288‑2613423 | aaTGAGTCCAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:1654546/70‑1 (MQ=255) aGTCCAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCa > 1:3417917/1‑71 (MQ=255) gTCCAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCAt > 1:3248121/1‑71 (MQ=255) cAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:2182334/62‑1 (MQ=255) cAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:1495542/62‑1 (MQ=255) cAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:326935/62‑1 (MQ=255) cAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGc > 1:43617/1‑70 (MQ=255) aGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGGCTGATATTTCGCCAATGGTTCATAAACCATGctc > 1:425146/1‑71 (MQ=255) aGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGctc > 1:2599146/1‑71 (MQ=255) aGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGctc > 1:1488608/1‑71 (MQ=255) aGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTAATAAACCATGctc > 1:3276771/1‑71 (MQ=255) gCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:1946326/60‑1 (MQ=255) cTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg > 1:1039907/1‑71 (MQ=255) cTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg < 1:3628020/71‑1 (MQ=255) cTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg > 1:2292678/1‑71 (MQ=255) tttttGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTc < 1:2742664/55‑1 (MQ=255) tttGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCAt > 1:3509743/1‑55 (MQ=255) tttGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCAt > 1:1191026/1‑55 (MQ=255) tttGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTgg < 1:612409/69‑1 (MQ=255) tttGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTgg < 1:1595720/69‑1 (MQ=255) tGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCg > 1:3491156/1‑70 (MQ=255) tGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCg > 1:1299683/1‑70 (MQ=255) ccagccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATa < 1:787046/52‑1 (MQ=255) gccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACAt > 1:1087690/1‑70 (MQ=255) gccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATc > 1:2432081/1‑71 (MQ=255) gccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATc > 1:2210839/1‑71 (MQ=255) ccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCt > 1:1683828/1‑71 (MQ=255) ccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCt > 1:1489860/1‑71 (MQ=255) ccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCt > 1:470948/1‑71 (MQ=255) ccaCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCt > 1:2139831/1‑71 (MQ=255) caCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTg < 1:1717989/71‑1 (MQ=255) caCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTg > 1:1275509/1‑71 (MQ=255) caCGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTg > 1:2263756/1‑71 (MQ=255) cGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGt > 1:1200439/1‑71 (MQ=255) cGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGt > 1:2035565/1‑71 (MQ=255) gggATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGtt < 1:3686179/71‑1 (MQ=255) ggATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGt > 1:1332339/1‑69 (MQ=255) ggATCACCGAACCGTCTGCCTGATATTTCACAAATGGTTCATAAACCATGCTCTGGGCGAACATCTgg > 1:2484173/1‑68 (MQ=255) aTCACCGAACCGTCTGCATGATATTTCACCTATGGTTGATAAACCATGCTCTGGGCg < 1:3563045/57‑1 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACGAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGc > 1:1360293/1‑71 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATaa < 1:2135922/41‑1 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATaa < 1:354916/41‑1 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATaa < 1:1911124/41‑1 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGctct > 1:1817510/1‑51 (MQ=255) tCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGc < 1:2785865/71‑1 (MQ=255) aCCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg < 1:1042348/50‑1 (MQ=255) aCCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg < 1:3702185/50‑1 (MQ=255) aCCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTg < 1:3201116/50‑1 (MQ=255) aCCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCgt > 1:1240273/1‑71 (MQ=255) gAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTa < 1:492660/71‑1 (MQ=255) aaCCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTaa < 1:2371425/71‑1 (MQ=255) ccGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAg > 1:485053/1‑71 (MQ=255) gTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGt > 1:1107649/1‑71 (MQ=255) gTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGt < 1:1389161/71‑1 (MQ=255) gTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGt < 1:1483438/71‑1 (MQ=255) tCTGCCTGATATTTCACCAATGGTTCATAAACCATGctc < 1:2138091/39‑1 (MQ=255) tCTGCCTGATATTTCACCAATGGTTCATAAACCATGctc < 1:714173/39‑1 (MQ=255) tCTGCCTGATATTTCACCAATGGTTCATAAACCATGctc < 1:2861086/39‑1 (MQ=255) tCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTaaa > 1:1756983/1‑67 (MQ=255) tCTGCATGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTg < 1:2442565/71‑1 (MQ=255) cTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACa > 1:50966/1‑48 (MQ=255) cTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACAt > 1:2664608/1‑49 (MQ=255) gCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAg > 1:1783139/1‑55 (MQ=255) ccTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGc < 1:1387350/68‑1 (MQ=255) tGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGtt > 1:1053396/1‑71 (MQ=255) tGATATTTCACAAATGGTTCATAAACCATGCTCTGGGCGAACAGCTGGTTAGGCGTGTAAAGGAGCGGGtt > 1:2005399/1‑71 (MQ=255) atatTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAg < 1:2052466/50‑1 (MQ=255) tatTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAg > 1:2880481/1‑71 (MQ=255) tatTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAg > 1:2879362/1‑71 (MQ=255) atTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTaa < 1:2897382/56‑1 (MQ=255) atTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAg < 1:2960620/70‑1 (MQ=255) tttCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAg < 1:1534288/69‑1 (MQ=255) tttCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTg < 1:2250384/71‑1 (MQ=255) cACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGc < 1:3695774/70‑1 (MQ=255) ccAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAg > 1:2888484/1‑52 (MQ=255) ccAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAg > 1:2097863/1‑52 (MQ=255) ccAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCg < 1:752397/71‑1 (MQ=255) ccAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCg < 1:1718329/71‑1 (MQ=255) cAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCg > 1:189312/1‑70 (MQ=255) caTGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgt < 1:2421532/43‑1 (MQ=37) caTGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgt < 1:655967/43‑1 (MQ=37) caTGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgt < 1:2969160/43‑1 (MQ=37) caTGGTTTATGAACCATGCTCTGCGCGAACATCTGGTTAGGCgt < 1:3313638/43‑1 (MQ=25) aaTGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTgg > 1:3316838/1‑65 (MQ=255) aaTGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTgg > 1:1805088/1‑65 (MQ=255) aaTGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTgg > 1:3312446/1‑65 (MQ=255) aaTGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGa > 1:2803330/1‑70 (MQ=255) aTGGTTGATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGGGGGTTTAGTGGCCCGACa < 1:1338723/71‑1 (MQ=255) tGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgtgt > 1:2762992/1‑44 (MQ=38) tGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgtgt > 1:496951/1‑44 (MQ=38) tGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgtgt > 1:2792432/1‑44 (MQ=38) tGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgtgt > 1:766854/1‑44 (MQ=38) tGGTTTATGAACCATGCTCTGGGCGAACATCTGGTTAGGCgtgt > 1:2797915/1‑44 (MQ=38) tGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACAt < 1:1062328/71‑1 (MQ=255) tGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACAt < 1:771544/71‑1 (MQ=255) ggTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACAt > 1:3699443/1‑70 (MQ=255) ttCATAAACCATGCGCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGt < 1:3538156/58‑1 (MQ=255) cATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGtt < 1:303156/52‑1 (MQ=255) cATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGtt < 1:2250764/52‑1 (MQ=255) cATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACATTCAc < 1:3275813/70‑1 (MQ=255) | AATGAGTCCAGCTTTTTGCCAGCCACGGGATCACCGAACCGTCTGCCTGATATTTCACCAATGGTTCATAAACCATGCTCTGGGCGAACATCTGGTTAGGCGTGTAAAGGTGCGGGTTTAGTGGCCCGACATTCAC > Exported/2613288‑2613423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |