Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,625,149 G→A 35.2% R16H (CGT→CAT)  ftsX → predicted transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,625,1490GA35.2% 76.2 / 68.5 71R16H (CGT→CAT) ftsXpredicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (19/27);  new base A (14/11);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 3.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAACGC  >  Exported/2625083‑2625213
                                                                  |                                                                
cTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCtt                                                              <  1:1144298/71‑1 (MQ=255)
cTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCtt                                                              <  1:682085/71‑1 (MQ=255)
cTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCtt                                                              <  1:2409808/71‑1 (MQ=255)
cTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCtt                                                              <  1:1669923/71‑1 (MQ=255)
   gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg                                                             <  1:3623984/69‑1 (MQ=255)
   gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg                                                             <  1:3304523/69‑1 (MQ=255)
   gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCt                                                               >  1:1342592/1‑67 (MQ=255)
   gCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCt                                                               >  1:1953631/1‑67 (MQ=255)
    cATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCTATCATATTCGGCAGTTTGGCGGGCGTCTTg                                                             <  1:3215687/68‑1 (MQ=255)
    cATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg                                                             <  1:2379569/68‑1 (MQ=255)
     aTGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCg                                                         >  1:1068300/1‑71 (MQ=255)
      tGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt                                                           >  1:2052070/1‑68 (MQ=255)
      tGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGAt                                                           >  1:3112897/1‑68 (MQ=255)
      tGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGc                                                        <  1:3487935/71‑1 (MQ=255)
      tGGAGGCGTGGGCCATGAATAAGAGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGa                                                            >  1:2737740/1‑67 (MQ=255)
          ggCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTcc                                                    <  1:2277731/71‑1 (MQ=255)
          ggCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTcc                                                    <  1:2156771/71‑1 (MQ=255)
           gCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCg                                                   <  1:3383654/71‑1 (MQ=255)
           gCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCg                                                   <  1:3387564/71‑1 (MQ=255)
           gCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCg                                                   <  1:2263194/71‑1 (MQ=255)
              tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaa                                                <  1:3308893/71‑1 (MQ=255)
              tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaa                                                <  1:3418863/71‑1 (MQ=255)
              tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaa                                                <  1:3573251/71‑1 (MQ=255)
              tGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaa                                                <  1:2019825/71‑1 (MQ=255)
               gggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCtt                                                      >  1:2534334/1‑64 (MQ=255)
                ggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGAt                                                           >  1:1960844/1‑58 (MQ=255)
                ggCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGAt                                                           >  1:1377848/1‑58 (MQ=255)
                  ccATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTaaa                                               >  1:3625313/1‑68 (MQ=255)
                  ccATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcg                                            >  1:903319/1‑71 (MQ=255)
                  ccATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcg                                            <  1:1246021/71‑1 (MQ=255)
                  ccATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcg                                            <  1:1676624/71‑1 (MQ=255)
                   cATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCt                                                               <  1:3385858/51‑1 (MQ=255)
                    aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggt                                          <  1:1669772/71‑1 (MQ=255)
                    aTGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggt                                          >  1:1661566/1‑71 (MQ=255)
                     tGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTg                                                             <  1:954598/51‑1 (MQ=255)
                      gAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcg                                        >  1:2869612/1‑71 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc                                         <  1:3733678/69‑1 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc                                         <  1:3461085/69‑1 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc                                         <  1:2255262/69‑1 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc                                         <  1:204773/69‑1 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATc                                                                >  1:754847/1‑46 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATc                                                                >  1:3488364/1‑46 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcgg                                       >  1:302083/1‑71 (MQ=255)
                       aaTAAGCGCGATGCAATCAATCATAATCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcgg                                       <  1:3262742/71‑1 (MQ=255)
                        aTAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTcggc                                      <  1:2062330/71‑1 (MQ=255)
                           aGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCggcggc                                   >  1:2466012/1‑71 (MQ=255)
                             cgcgATGCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCt                                  >  1:2703665/1‑70 (MQ=255)
                                  tGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGc                             >  1:1701523/1‑70 (MQ=255)
                                  tGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCGTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGc                             >  1:3661754/1‑70 (MQ=255)
                                   gCAATCAATCATATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAAtcggtcgg                                       >  1:2408049/1‑59 (MQ=255)
                                      atcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                        >  1:1581186/1‑71 (MQ=255)
                                      atcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                        >  1:2623943/1‑71 (MQ=255)
                                      atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                        <  1:3580831/71‑1 (MQ=255)
                                      atcaatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcgg                        <  1:1656835/71‑1 (MQ=255)
                                       tcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATc                                                          >  1:101985/1‑36 (MQ=255)
                                       tcaatcaTATTCGGCAGTTTGGCGGGCGTCTTGATc                                                          >  1:3028004/1‑36 (MQ=255)
                                        caatcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtc                                         >  1:3150350/1‑52 (MQ=255)
                                        caatcaTATTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGAcggc                       >  1:373310/1‑70 (MQ=255)
                                          atcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAg                               >  1:1576353/1‑60 (MQ=255)
                                           tcaTATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTc                   >  1:1870206/1‑71 (MQ=255)
                                             atatTCGGCAGTTTGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt                 <  1:2306461/71‑1 (MQ=255)
                                                ttCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGc                             <  1:1739718/56‑1 (MQ=255)
                                                 tCGGCAGTTTGGCGGGCGTCTTGATAGCTTCCGTAAATCGGTCGGCGGCTCAggg                             >  1:2414246/1‑54 (MQ=255)
                                                     cAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAAtcggt                                          >  1:228107/1‑38 (MQ=255)
                                                       gTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaa       <  1:2840038/71‑1 (MQ=255)
                                                       gTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaa       <  1:1616376/71‑1 (MQ=255)
                                                       gTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCa        >  1:1234655/1‑70 (MQ=255)
                                                        tttGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtcg                                        <  1:1576267/37‑1 (MQ=255)
                                                        tttGGCGGGCGTCTTGATCGCTTCCGTAAAtcggtcg                                        <  1:2458810/37‑1 (MQ=255)
                                                          tGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGt                 >  1:177710/1‑58 (MQ=255)
                                                          tGGCGGGCATCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCaaaa     <  1:1045719/70‑1 (MQ=255)
                                                           ggcgggcgTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAAc    <  1:2556944/70‑1 (MQ=255)
                                                            gcgggcaTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAAcgc  <  1:1603770/71‑1 (MQ=255)
                                                            gcgggcaTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAAcgc  <  1:196587/71‑1 (MQ=255)
                                                                  |                                                                
CTTGCATGGAGGCGTGGGCCATGAATAAGCGCGATGCAATCAATCATATTCGGCAGTTTGGCGGGCGTCTTGATCGCTTCCGTAAATCGGTCGGCGGCTCAGGCGACGGCGGTCGTAACGCACCAAAACGC  >  Exported/2625083‑2625213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: