| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,639,772 | T→G | 9.2% | C233G (TGT→GGT) | ugpQ → | glycerophosphodiester phosphodiesterase, cytosolic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,639,772 | 0 | T | G | 9.2% | 223.2 / 18.5 | 87 | C233G (TGT→GGT) | ugpQ | glycerophosphodiester phosphodiesterase, cytosolic |
| Reads supporting (aligned to +/- strand): ref base T (37/42); new base G (6/2); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
GTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCA > Exported/2639709‑2639836 | gTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCg < 1:3151088/70‑1 (MQ=255)gTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:2054930/1‑71 (MQ=255)gTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:1893877/1‑71 (MQ=255) ttATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGAt > 1:1424794/1‑70 (MQ=255) tataCCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATg < 1:1763242/70‑1 (MQ=255) taCCGTCAATAAACCCCAGCGCTCAGCAGACTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGa < 1:2156367/71‑1 (MQ=255) tCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGAc > 1:1529102/1‑71 (MQ=255) tCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGAc > 1:2152719/1‑71 (MQ=255) tCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGAc > 1:956289/1‑71 (MQ=255) cAATAAACCCCAGCGCGCAGCAGGGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACg > 1:2408062/1‑71 (MQ=255) aaTAAACCCCAGCGCGCAGCAGAGGTGCTGCGCTGGGGTGTGGATTGCATCTGt < 1:3246523/54‑1 (MQ=255) aTAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTg < 1:1719584/71‑1 (MQ=255) aaaCCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGt < 1:79178/51‑1 (MQ=255) aaCCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGAtt > 1:731269/1‑71 (MQ=255) aCCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTg < 1:38378/71‑1 (MQ=255) gcgcAGCAGAGTTGCTGCGCTGTGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAAc > 1:2358279/1‑71 (MQ=255) gcgcAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAAc < 1:1525095/71‑1 (MQ=255) cgcAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTAc < 1:412553/42‑1 (MQ=255) cagcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACtt < 1:996165/70‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa < 1:3342294/71‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa < 1:1141894/71‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa < 1:1089598/71‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa < 1:1594859/71‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa > 1:1934246/1‑71 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa < 1:1462127/71‑1 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa > 1:181197/1‑71 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTa > 1:1257570/1‑71 (MQ=255) agcagAGTTGCTGCGCTGGGGTGTGGATCGCATCGGTACAGATg > 1:59702/1‑44 (MQ=25) agcagAGTTGCTGCGCTGGGGTGTGGATCGCATCGGTACAGATg > 1:2205999/1‑44 (MQ=25) agcagAGTTGCTGCGCTGGGGTGTGGATCGCATCGGTACAGATg > 1:2377748/1‑44 (MQ=25) agcagAGTTGCTGCGCTGGGGTGTGGATCGCATCGGTACAGATg > 1:2491028/1‑44 (MQ=25) gcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGt < 1:2762977/60‑1 (MQ=255) gcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGt < 1:343533/60‑1 (MQ=255) gcagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGa < 1:3591616/64‑1 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:3475367/1‑40 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:2061878/1‑40 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:1305446/1‑40 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:259333/1‑40 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGa > 1:2541757/1‑40 (MQ=255) cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:3598848/71‑1 (MQ=255) ttGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCa < 1:3610186/71‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGAc < 1:684557/41‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCg < 1:446929/53‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCg < 1:2946055/53‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCg < 1:3249729/53‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGa < 1:1745254/54‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGa < 1:3044968/54‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGa < 1:3107011/54‑1 (MQ=255) tgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAg > 1:1211886/1‑71 (MQ=255) gcgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg > 1:437439/1‑61 (MQ=255) gcgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGt > 1:3374671/1‑71 (MQ=255) gcgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGt > 1:2922585/1‑71 (MQ=255) cgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCTCAATAg < 1:4002/69‑1 (MQ=255) gcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAAc > 1:1911871/1‑53 (MQ=255) gcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGttt > 1:2135097/1‑71 (MQ=255) gggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTc < 1:16025/69‑1 (MQ=255) gggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCaa < 1:995802/71‑1 (MQ=255) ggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGtt > 1:3635684/1‑65 (MQ=255) tgtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:827376/52‑1 (MQ=255) tgtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:68800/52‑1 (MQ=255) tgtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:1901731/52‑1 (MQ=255) tgtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:2739948/52‑1 (MQ=255) tgtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACg < 1:2755108/52‑1 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATg > 1:2627419/1‑71 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATg > 1:386677/1‑71 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATg > 1:3323047/1‑71 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATg > 1:592000/1‑71 (MQ=255) aTTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGt < 1:2496893/71‑1 (MQ=255) aTTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGt < 1:188041/71‑1 (MQ=255) ttGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGt > 1:716917/1‑70 (MQ=255) ttGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGt > 1:2217813/1‑70 (MQ=255) tcgCATCGGTACAGATGCGATTGACGTGATTGGTCCGAACtt < 1:1240878/40‑1 (MQ=25) tcgCATCGGTACAGATGCGATTGACGTGATTGGTCCGAACtt < 1:2179467/40‑1 (MQ=25) tcgCATCGGTACAGATGCAATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:3424455/3‑51 (MQ=25) tcgCATCGGTACAGATGCAATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:1341274/3‑51 (MQ=25) gCATCTGTACCGATGCGTTTGACGTGATTGGTCCGAACTTTACGGCCCAATTGttt > 1:3105722/1‑56 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa < 1:881114/70‑1 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa < 1:2011086/70‑1 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa > 1:2015295/1‑70 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa < 1:3257172/70‑1 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGGCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCa < 1:2870965/70‑1 (MQ=255) aTCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACgg < 1:2495285/43‑1 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:2231048/1‑45 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:2483508/1‑45 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:3024099/1‑45 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCa > 1:3182988/1‑45 (MQ=255) cTGTACCGATGCGATTGACGTAATTGGTCCGAACTTTACGGCCCa > 1:2418452/1‑45 (MQ=255) | GTTTATACCGTCAATAAACCCCAGCGCGCAGCAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCA > Exported/2639709‑2639836 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |