| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,714,239 | C→A | 48.9% | R41L (CGC→CTC) | nirB ← | nitrite reductase, large subunit, NAD(P)H‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,714,239 | 0 | C | A | 48.9% | 23.1 / 56.0 | 47 | R41L (CGC→CTC) | nirB | nitrite reductase, large subunit, NAD(P)H‑binding |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base A (11/12); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
AGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGATAA > Exported/2714179‑2714308 | aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATgcgc > 1:2616183/1‑63 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:931059/1‑70 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:2717227/1‑70 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:2860640/1‑70 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:3135195/1‑70 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:3154311/1‑70 (MQ=255)aGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGttctt > 1:1296485/1‑70 (MQ=255) ctTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca > 1:2801897/1‑68 (MQ=255) ctTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca > 1:2935090/1‑68 (MQ=255) ggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGtt < 1:1435502/59‑1 (MQ=255) ggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGtt < 1:3536905/59‑1 (MQ=255) ggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGtt < 1:2773235/59‑1 (MQ=255) ggcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaaaa > 1:2610664/1‑71 (MQ=255) gcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGtt < 1:3704656/58‑1 (MQ=255) gcggTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAAc < 1:1838903/71‑1 (MQ=255) gtgtGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACgg > 1:2503122/1‑70 (MQ=255) gtggtgAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTaa > 1:3197604/1‑71 (MQ=255) agagaTGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:468762/70‑1 (MQ=255) agagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:377969/70‑1 (MQ=255) agagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCa < 1:1536371/70‑1 (MQ=255) gagaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCa > 1:3539199/1‑69 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAg < 1:1364965/71‑1 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAg > 1:2589207/1‑71 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca < 1:2986180/50‑1 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca < 1:2909960/50‑1 (MQ=255) agaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca < 1:3374875/50‑1 (MQ=255) gaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGt < 1:935190/71‑1 (MQ=255) gaAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGt < 1:1525163/71‑1 (MQ=255) aaGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt > 1:3365810/1‑71 (MQ=255) aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTg < 1:2173391/71‑1 (MQ=255) aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTg < 1:445373/71‑1 (MQ=255) aGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGTGGTTCTTCACAGAAAACGGTAATATCAAAGtt < 1:137717/70‑1 (MQ=255) gTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTgg < 1:2271085/71‑1 (MQ=255) tAAGACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGc < 1:1716658/71‑1 (MQ=255) gACGAGAGGTGTACGCGGTCATAAGCGATGAGCGGTTCTTca > 1:3344969/1‑42 (MQ=255) agagGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGaaaa > 1:1623316/1‑45 (MQ=255) gagGTGTACGCGGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAg < 1:1967647/71‑1 (MQ=255) tACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTa < 1:1076827/70‑1 (MQ=255) cgcgGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATc > 1:1487704/1‑70 (MQ=255) cgcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAGGTTgc > 1:3459390/1‑53 (MQ=255) cgcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGtt > 1:2435371/1‑52 (MQ=255) cgcgGTCATAAGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGG‑CGCATCAGATTTATc > 1:1583003/1‑69 (MQ=255) aaGCGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGa > 1:2643771/1‑55 (MQ=255) aGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCt < 1:3142656/70‑1 (MQ=255) cGATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCg < 1:837945/71‑1 (MQ=255) gATGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCa < 1:3523530/58‑1 (MQ=255) aTGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:1450492/1‑59 (MQ=255) aTGAGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCaag > 1:122803/1‑59 (MQ=255) cgcgGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGATaa < 1:2828941/70‑1 (MQ=255) cgcgGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGATaa < 1:1121440/70‑1 (MQ=255) | AGCTCTTCGGCGGTGTGGTGAGAGAAGTAAGACGAGAGGTGTACGCGGTCATAAGCGATGCGCGGTTCTTCACAGAAAACGGTAATATCAAAGTTGGCCGCATCAGATTTATCAAGAAGATCTTCGATAA > Exported/2714179‑2714308 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |