| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,736,671 | T→C | 57.5% | F605F (TTT→TTC) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,736,671 | 0 | T | C | 57.5% | 35.4 / 90.6 | 73 | F605F (TTT→TTC) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base T (14/17); new base C (19/23); total (33/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01 | |||||||||||
ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACC > Exported/2736604‑2736740 | aCCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCttt > 1:3377618/1‑68 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGa < 1:3181238/71‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGa < 1:2776423/71‑1 (MQ=255) ccATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGa < 1:2300109/71‑1 (MQ=255) cATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGaa < 1:2615197/71‑1 (MQ=255) aCGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGc < 1:1897531/70‑1 (MQ=255) gTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGa > 1:1546699/1‑70 (MQ=255) tcctcTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGa < 1:3433950/58‑1 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTc > 1:1227680/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTc > 1:968382/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTc > 1:2852884/1‑71 (MQ=255) ctctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTc > 1:1524039/1‑71 (MQ=255) tctGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAg < 1:3187200/67‑1 (MQ=255) tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGc > 1:3182501/1‑71 (MQ=255) tGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCttc > 1:620241/1‑69 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGaaa < 1:771227/53‑1 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCtt < 1:1830009/71‑1 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCtt > 1:2351908/1‑71 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCtt < 1:1433779/71‑1 (MQ=255) aaCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCtt < 1:433635/71‑1 (MQ=255) aCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCtt < 1:2291080/70‑1 (MQ=255) aCTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTg < 1:437374/71‑1 (MQ=255) tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAg < 1:743721/60‑1 (MQ=255) tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAg < 1:3670699/60‑1 (MQ=255) tGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAg > 1:685702/1‑71 (MQ=255) tGGCGATTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCaa > 1:546430/1‑46 (MQ=38) tttAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGa < 1:3650605/49‑1 (MQ=255) tttAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGa < 1:415516/49‑1 (MQ=255) ttAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAAcc > 1:1565899/1‑52 (MQ=255) ttAAACTGGATGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGAt > 1:2076931/1‑70 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAg > 1:1459782/1‑53 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAg > 1:2033001/1‑53 (MQ=255) tAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGa < 1:2188254/68‑1 (MQ=255) aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCAt > 1:2454162/1‑70 (MQ=255) aaCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATg < 1:722417/71‑1 (MQ=255) aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGa < 1:3604941/71‑1 (MQ=255) aCTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCAt > 1:497630/1‑69 (MQ=255) gctgctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGa < 1:1497150/67‑1 (MQ=255) gctgctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTCCTGCTTGAGCCGATCATGa < 1:1418699/67‑1 (MQ=255) ctTCTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTg < 1:642329/69‑1 (MQ=255) tCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAg > 1:3647517/1‑70 (MQ=255) cTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAg > 1:1854589/1‑69 (MQ=255) cTATCGCCTTTAAAGAAGGCTTTAAAAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAg > 1:1528934/1‑69 (MQ=255) cTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGttg > 1:193638/1‑41 (MQ=38) cTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTc > 1:128096/1‑42 (MQ=255) cTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTc > 1:2538731/1‑42 (MQ=255) cTATCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAg < 1:2981759/69‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAg < 1:2306671/69‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTa < 1:1331567/68‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTa < 1:1925167/68‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTa < 1:2552403/68‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAg < 1:695636/69‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAg < 1:67445/69‑1 (MQ=255) tCGCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAg < 1:2047577/69‑1 (MQ=255) gCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGcc < 1:1757991/47‑1 (MQ=255) gCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGcc < 1:2226219/47‑1 (MQ=255) gCCTTTAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAAc > 1:3341979/1‑71 (MQ=255) tttAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTc > 1:994132/1‑70 (MQ=255) ttAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGa < 1:169597/64‑1 (MQ=255) tAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCg > 1:2969726/1‑70 (MQ=255) tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGtt > 1:150364/1‑55 (MQ=255) tAAAGAAGGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGtt > 1:3571980/1‑55 (MQ=255) aagaagGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa < 1:682373/71‑1 (MQ=255) aagaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAgg < 1:3221660/51‑1 (MQ=255) aagaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAgg < 1:3054502/51‑1 (MQ=255) agaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt < 1:3236325/64‑1 (MQ=255) gaagGCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGaa > 1:2991869/1‑69 (MQ=255) agGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAagag > 1:2532032/1‑70 (MQ=255) ggCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:670263/1‑40 (MQ=255) ggCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:386990/1‑40 (MQ=255) ggCTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCa > 1:429989/1‑40 (MQ=255) gCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt < 1:1874375/59‑1 (MQ=255) gCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACt < 1:511920/59‑1 (MQ=255) cTTCAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCgg > 1:2407531/1‑62 (MQ=255) ttAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACAcc > 1:1381012/1‑71 (MQ=255) | ACCATGACGTTGACTCCTCTGAACTGGCGTTTAAACTGGCTGCTTCTATCGCCTTTAAAGAAGGCTTTAAGAAAGCGAAACCAGTTCTGCTTGAGCCGATCATGAAGGTTGAAGTAGAAACTCCGGAAGAGAACACC > Exported/2736604‑2736740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |