| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,745,295 | C→A | 8.8% | P52Q (CCG→CAG) | rpsN → | 30S ribosomal subunit protein S14 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,745,295 | 0 | C | A | 8.8% | 149.9 / 2.1 | 45 | P52Q (CCG→CAG) | rpsN | 30S ribosomal subunit protein S14 |
| Reads supporting (aligned to +/- strand): ref base C (29/12); new base A (2/2); total (31/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGATGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCG > Exported/2745236‑2745364 | tGATGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGAt < 1:325228/67‑1 (MQ=255)tGATGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTc < 1:1065611/69‑1 (MQ=255)tGATGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTc < 1:2231814/69‑1 (MQ=255) aTGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGc > 1:1733332/1‑71 (MQ=255) gtgAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGccc > 1:2843718/1‑71 (MQ=255) aaCGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGtc > 1:1421486/1‑71 (MQ=255) gCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCg < 1:3060806/71‑1 (MQ=255) cTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGt > 1:3250168/1‑71 (MQ=255) cTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGt > 1:3250710/1‑71 (MQ=255) cTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGt < 1:3344434/71‑1 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGt > 1:3695944/1‑50 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAg > 1:3167260/1‑71 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAg > 1:1291003/1‑71 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAg > 1:2111005/1‑71 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAg > 1:2774196/1‑71 (MQ=255) ccGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCACGTCTCGTCAg > 1:729515/1‑71 (MQ=255) cgacgaAGATCGTTGGAACGCTGTTATCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGc > 1:1111057/1‑71 (MQ=255) gaAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTc > 1:3106790/1‑64 (MQ=255) gaAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTaa > 1:2461660/1‑71 (MQ=255) gATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCg > 1:3165766/1‑71 (MQ=255) tCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCt > 1:891060/1‑71 (MQ=255) ggAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCg > 1:2385324/1‑52 (MQ=255) ggAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCg > 1:3390668/1‑70 (MQ=255) cTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCaaa > 1:232793/1‑69 (MQ=255) cTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACa < 1:1590882/71‑1 (MQ=255) tGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGt > 1:2589759/1‑46 (MQ=255) tGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGt > 1:1448198/1‑46 (MQ=255) ttCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGgt > 1:1073268/1‑71 (MQ=255) ttCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGgt > 1:1708959/1‑71 (MQ=255) ctcAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCt < 1:3551618/55‑1 (MQ=255) ctcAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGgtcg > 1:672346/1‑71 (MQ=255) tcACGCGGCAGAGTCTGCAGCTTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGgt > 1:2504158/1‑68 (MQ=25) tcACGCGGCAGAGTCTGCAGCTTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGgt > 1:1172617/1‑68 (MQ=25) cAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGt > 1:414556/1‑58 (MQ=255) aGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCg < 1:2858892/71‑1 (MQ=255) aGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCg < 1:465498/71‑1 (MQ=255) cTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCa > 1:32702/1‑71 (MQ=255) ggCAGAGTCTGCAGCTTGAGAACAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCAt < 1:1666566/70‑1 (MQ=25) ggCAGAGTCTGCAGCTTGAGAACAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCAt < 1:2290899/70‑1 (MQ=25) gACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCAt > 1:789707/1‑67 (MQ=255) gACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATg > 1:1130196/1‑68 (MQ=255) gACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGtt < 1:1631151/71‑1 (MQ=255) gACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGtt > 1:3104119/1‑71 (MQ=255) aCTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGtt < 1:185207/70‑1 (MQ=255) ctctGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTc < 1:3103177/71‑1 (MQ=255) ccGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTg < 1:3219184/69‑1 (MQ=255) ccGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCg < 1:2717661/71‑1 (MQ=255) ccGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCg < 1:1669543/71‑1 (MQ=255) ccGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCg > 1:2212623/1‑71 (MQ=255) cgcgTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCg > 1:723952/1‑70 (MQ=255) | TGATGTGAACGCTTCCGACGAAGATCGTTGGAACGCTGTTCTCAAGCTGCAGACTCTGCCGCGTGATTCCAGCCCGTCTCGTCAGCGTAACCGCTGCCGTCAAACAGGTCGTCCGCATGGTTTCCTGCG > Exported/2745236‑2745364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |