| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,823,561 | A→C | 60.6% | intergenic (+306/+652) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,823,561 | 0 | A | C | 60.6% | 139.7 / 100.2 | 109 | intergenic (+306/+652) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (15/28); new base C (26/40); total (41/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 120 total aligned reads displayed.
TTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > Exported/2823492‑2823627 | aaaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc < 1:1570985/70‑1 (MQ=255) aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:1036278/71‑1 (MQ=255) aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:196280/71‑1 (MQ=255) aaaaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:1953000/71‑1 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:426767/1‑70 (MQ=255) aaaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACgggg < 1:1523505/71‑1 (MQ=255) aaaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa < 1:459520/71‑1 (MQ=255) aaaTCTACAAACCAACGCAACACAATTCATGCCCAGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:3275463/1‑69 (MQ=255) aaTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGa < 1:2489265/70‑1 (MQ=255) aTCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAAc > 1:2913080/1‑71 (MQ=255) tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCAc < 1:1174270/61‑1 (MQ=255) tACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACggg < 1:2206791/64‑1 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:3434228/68‑1 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:2557499/68‑1 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACgg < 1:3130077/68‑1 (MQ=255) cAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGgcgc < 1:3179479/71‑1 (MQ=255) aCCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTc > 1:473221/1‑70 (MQ=255) aCCAACGCAACACAATTCATGCACTGGCAGTATGTCACGTTCTCGCGTTTCTCaa > 1:898567/1‑55 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:3590782/1‑52 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1056391/1‑52 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:1928345/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:2664479/71‑1 (MQ=255) aaCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCAtt < 1:3331176/71‑1 (MQ=255) aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:3149285/1‑51 (MQ=255) aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:916323/1‑51 (MQ=255) aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1544231/1‑51 (MQ=255) aCGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTg < 1:580328/71‑1 (MQ=255) gCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAg < 1:3108039/71‑1 (MQ=255) gCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACg > 1:798001/1‑51 (MQ=255) aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCt > 1:1356321/1‑61 (MQ=255) aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCt > 1:2136624/1‑61 (MQ=255) aaCACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAgg > 1:3133376/1‑70 (MQ=255) acacAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:3683298/49‑1 (MQ=255) acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgggg > 1:3270017/1‑49 (MQ=255) acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgggg > 1:985826/1‑49 (MQ=255) acaATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACggg > 1:1032228/1‑48 (MQ=255) aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGa < 1:2094606/66‑1 (MQ=255) aaTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGa < 1:2002410/66‑1 (MQ=255) aTTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACgg < 1:876964/44‑1 (MQ=255) ttCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:2125330/1‑71 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:2145697/1‑39 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:268267/1‑39 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:687826/1‑39 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGaa > 1:1297122/1‑39 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:845450/62‑1 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGATCGGGGAACGGCGCTCCATTGa < 1:1304714/60‑1 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGa < 1:2405933/60‑1 (MQ=255) tCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGa < 1:2730958/60‑1 (MQ=255) aTGCCCTGGCAGTATGTCACGTTCTCGGGTTTCTGCACGGGGAACGGCGCTCCATTGAGGaa > 1:2283553/1‑62 (MQ=255) aTGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGaa > 1:3715896/1‑62 (MQ=255) aTGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcattcat < 1:2212346/71‑1 (MQ=255) gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:1862397/50‑1 (MQ=255) gCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTc < 1:1494944/50‑1 (MQ=255) cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1394440/1‑70 (MQ=255) cccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:1081670/1‑70 (MQ=255) ccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcattca < 1:2670769/66‑1 (MQ=255) ccTGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:3281668/1‑69 (MQ=255) ccTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCataa > 1:533356/1‑69 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACgg < 1:92054/41‑1 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg > 1:393815/1‑51 (MQ=255) tGGCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTg > 1:1204238/1‑51 (MQ=255) ggCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCa > 1:2431735/1‑47 (MQ=255) ggCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa > 1:2359772/1‑70 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcatt > 1:964902/1‑60 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca < 1:2897445/58‑1 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGaa < 1:2209736/70‑1 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:736684/69‑1 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGa < 1:1827862/69‑1 (MQ=255) gCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGt < 1:2594546/56‑1 (MQ=255) gTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtc < 1:864108/54‑1 (MQ=255) gTATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtc < 1:830625/54‑1 (MQ=255) tATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCa < 1:291537/71‑1 (MQ=255) tATGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCa < 1:2854270/71‑1 (MQ=255) aTGTCACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCAt > 1:2561140/1‑71 (MQ=255) gTCACGTTCTCGCGTTTCTGCACGTGGAACGGCGCTCCATTGAGGAAGtc < 1:2616992/50‑1 (MQ=255) cACGTTCTCGCGTTTGTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:2176642/1‑50 (MQ=255) cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:2271518/1‑50 (MQ=255) cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:1073937/1‑50 (MQ=255) cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtcat > 1:1189566/1‑50 (MQ=255) cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGtca > 1:416504/1‑49 (MQ=255) cACGTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg < 1:2308150/59‑1 (MQ=255) aCGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaa < 1:69990/71‑1 (MQ=255) cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:1646152/71‑1 (MQ=255) cGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaa < 1:1738819/71‑1 (MQ=255) gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaaa < 1:151660/71‑1 (MQ=255) gTTCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGaaaa < 1:2682894/71‑1 (MQ=255) ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAgg < 1:196758/39‑1 (MQ=255) ttCTCGCGTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAg < 1:2463737/71‑1 (MQ=255) ctcGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:1985728/1‑70 (MQ=255) ctcGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAgg > 1:2573960/1‑70 (MQ=255) gcgTTTCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAggg < 1:2072724/68‑1 (MQ=255) gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:1243953/1‑71 (MQ=255) gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:1265365/1‑71 (MQ=255) gTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatga > 1:2173912/1‑71 (MQ=255) tCTGCACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAatgatg < 1:3364975/70‑1 (MQ=255) cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAg > 1:842225/1‑44 (MQ=255) cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc < 1:2663418/70‑1 (MQ=255) cTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGc < 1:1964246/70‑1 (MQ=255) tGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata < 1:2612864/39‑1 (MQ=255) tGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCata < 1:2495626/39‑1 (MQ=255) | TTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCGTTTCTGAACGGGGAACGGCGCTCCATTGAGGAAGTCATTCATAATAGAAACTCATTCGAAAAGGGAATGATGC > Exported/2823492‑2823627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |