Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,852,261 T→C 9.4% H304R (CAC→CGC)  rsgA ← ribosome small subunit‑dependent GTPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,852,2610TC 9.4% 181.0 / 9.1 64H304R (CAC→CGC) rsgAribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base T (36/22);  new base C (2/4);  total (38/26)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

TCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGTT  >  Exported/2852192‑2852330
                                                                     |                                                                     
tCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCgt                                                                       <  1:1849199/70‑1 (MQ=255)
tCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCgt                                                                       <  1:1966881/70‑1 (MQ=255)
 ggAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCgtg                                                                      >  1:3422523/2‑70 (MQ=255)
 cGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCgtgt                                                                     >  1:540971/1‑71 (MQ=255)
     aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTg                                                                  >  1:1520900/1‑70 (MQ=255)
     aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGc                                                                 <  1:3528921/71‑1 (MQ=255)
     aCGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGc                                                                 <  1:3533269/71‑1 (MQ=255)
       gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               <  1:536184/71‑1 (MQ=255)
       gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               <  1:404548/71‑1 (MQ=255)
       gggTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               <  1:394875/71‑1 (MQ=255)
          tttCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAAt                                                              <  1:470501/69‑1 (MQ=255)
          tttCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAAt                                                              <  1:1860923/69‑1 (MQ=255)
              cgcgATTTTCCCTTACTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGat                                                        <  1:509343/71‑1 (MQ=255)
                 gATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt                                                     >  1:669150/1‑71 (MQ=255)
                   ttttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAttt                                                    >  1:1765263/1‑70 (MQ=255)
                     ttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTg                                                                  >  1:280034/1‑54 (MQ=255)
                     ttCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATc                                                             >  1:1534235/1‑59 (MQ=255)
                      tCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTaca                                                 <  1:953887/70‑1 (MQ=255)
                        ccTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTAcaca                                               <  1:1918157/70‑1 (MQ=255)
                         cTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt                                                     <  1:3677075/63‑1 (MQ=255)
                         cTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATAtt                                                     <  1:2885649/63‑1 (MQ=255)
                          ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               >  1:3486802/1‑52 (MQ=255)
                          ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               >  1:3193652/1‑52 (MQ=255)
                          ttCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCaa                                                               >  1:218970/1‑52 (MQ=255)
                           tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATGGTGTTTGCAATCGCGATATTTACACAGAcc                                           >  1:62465/1‑71 (MQ=255)
                           tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGatat                                                      >  1:1255918/1‑60 (MQ=255)
                           tCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTa                                                   >  1:2514641/1‑63 (MQ=255)
                            ccTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGAcc                                           >  1:1516278/1‑70 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCt                                          <  1:2318806/70‑1 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa                                         <  1:1726579/71‑1 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa                                         <  1:2742110/71‑1 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa                                         <  1:2686342/71‑1 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa                                         <  1:816498/71‑1 (MQ=255)
                             cTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTa                                         <  1:886755/71‑1 (MQ=255)
                                aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:3438348/1‑70 (MQ=255)
                                aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:3142763/1‑70 (MQ=255)
                                aaCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:3057364/1‑70 (MQ=255)
                                 aCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:3222504/1‑69 (MQ=255)
                                 aCCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:309502/1‑69 (MQ=255)
                                     cTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCa                                 >  1:3059887/1‑71 (MQ=255)
                                        cccGGATAGCGCAGCCCGGATCGGTATCGTCTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATg                               >  1:2482006/1‑70 (MQ=255)
                                        cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat                                                        <  1:581387/45‑1 (MQ=17)
                                        cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat                                                        <  1:520680/45‑1 (MQ=17)
                                        cccGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGat                                                        <  1:1957072/45‑1 (MQ=17)
                                        cccGGATAGCGCAGCCCGGATCGATATCGCGATAGCAAACACGat                                                        <  1:64711/45‑1 (MQ=12)
                                         ccGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATgg                              <  1:370204/70‑1 (MQ=255)
                                          cGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGatac                                                      >  1:3719780/1‑44 (MQ=9)
                                          cGGATAGCGCAGCCCGGATCGATATCGCGATTGCAAACACGatac                                                      >  1:2481723/1‑44 (MQ=9)
                                             aTAGCGGAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:478954/1‑57 (MQ=255)
                                             aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTaaa                                       >  1:1517284/1‑57 (MQ=255)
                                             aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTc                                  <  1:2735768/62‑1 (MQ=255)
                                             aTAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTc                                  <  1:1821846/62‑1 (MQ=255)
                                               aGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa                       >  1:3160072/1‑71 (MQ=255)
                                                        cGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt              >  1:2026721/1‑71 (MQ=255)
                                                        cGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt              >  1:1819061/1‑71 (MQ=255)
                                                          gATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt              >  1:3293946/1‑69 (MQ=255)
                                                          gATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCt              >  1:2752428/1‑69 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACATACCTAAATAGTCATGGAATTCGACAAAGCCCTGAg           <  1:390681/71‑1 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACaaa                   >  1:2872075/1‑63 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc               >  1:1657178/1‑67 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc               >  1:1081180/1‑67 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc               >  1:2059711/1‑67 (MQ=255)
                                                           aTCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGccc               >  1:287005/1‑67 (MQ=255)
                                                              ggTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGa        >  1:251895/1‑71 (MQ=255)
                                                                 aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt     >  1:232565/1‑71 (MQ=255)
                                                                 aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt     >  1:894164/1‑71 (MQ=255)
                                                                 aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt     >  1:1947891/1‑71 (MQ=255)
                                                                 aTCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGAttt     >  1:206698/1‑71 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAAt                           >  1:2329464/1‑46 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTc                         >  1:1779667/1‑48 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTc                         >  1:230874/1‑48 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa                       >  1:2852664/1‑50 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa                       >  1:794823/1‑50 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGa                       >  1:572891/1‑50 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGtt  <  1:1758270/71‑1 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGt   >  1:409249/1‑70 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTAGa                       >  1:1485525/1‑50 (MQ=255)
                                                                    gtgtTTGCAATCGCGATATTTACACAGACCTAAATAGTCATAGAATTCGACAAAGCCCTGAGTGATTTGt   <  1:3554812/70‑1 (MQ=255)
                                                                    gtgtTTGCAATCGAGATATTTACACAGACCTAAATAGTCATGGAATTCGa                       >  1:1488903/1‑50 (MQ=255)
                                                                     |                                                                     
TCGAAACGGGTTTCCGCGATTTTCCCTTCCTCAACCGCTTCCCGGATAGCGCAGCCCGGATCGGTATCGTGTTTGCAATCGCGATATTTACACAGACCTAAATAGTCATGGAATTCGACAAAGCCCTGAGTGATTTGTT  >  Exported/2852192‑2852330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: