Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,892,177 G→A 47.6% D278N (GAC→AAC)  ytfQ → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,892,1770GA47.6% 8.4 / 87.3 63D278N (GAC→AAC) ytfQpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (17/16);  new base A (21/9);  total (38/25)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC  >  Exported/2892111‑2892242
                                                                  |                                                                 
gCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGACGAATATGGCAGGTCCCGCCTTCAAcgc                                                               >  1:253698/1‑71 (MQ=255)
  gatgatGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCAAATATGGCAGGTCCCGCCTTCGACgcgc                                                             <  1:1458203/71‑1 (MQ=255)
     gatggatgGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTg                                                           >  1:2263692/1‑70 (MQ=255)
       tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                        <  1:3170661/71‑1 (MQ=255)
       tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                        <  1:2950030/71‑1 (MQ=255)
       tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                        <  1:2722233/71‑1 (MQ=255)
       tggatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGgag                                                        <  1:2312324/71‑1 (MQ=255)
         gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga                                                       >  1:3109195/1‑70 (MQ=255)
         gatggCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGgaga                                                       >  1:1016659/1‑70 (MQ=255)
             gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGTTCCCGCCTTCGACGCGCTGGAGAAATa                                                   <  1:1062231/70‑1 (MQ=255)
             gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa                                                   <  1:3466392/70‑1 (MQ=255)
             gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa                                                   <  1:1036881/70‑1 (MQ=255)
             gcgaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATa                                                   <  1:1723644/70‑1 (MQ=255)
               gaagcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACa                                                 >  1:383313/1‑70 (MQ=255)
                 agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaa                                               >  1:3735498/1‑70 (MQ=255)
                 agcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaa                                               >  1:3716202/1‑70 (MQ=255)
                  gcgaaCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaa                                             <  1:3578902/71‑1 (MQ=255)
                    gaaCCCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaa                                              >  1:3252216/1‑68 (MQ=255)
                      aCGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGAc                                         >  1:1028507/1‑71 (MQ=255)
                       cGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACg                                        >  1:900112/1‑71 (MQ=255)
                          cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa                                     >  1:487186/1‑71 (MQ=255)
                          cAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCa                                     >  1:371540/1‑71 (MQ=255)
                          aaGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCa                                     >  1:2622618/2‑71 (MQ=255)
                           aGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    <  1:1465471/71‑1 (MQ=255)
                            gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    >  1:1022778/1‑70 (MQ=255)
                            gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    >  1:2944146/1‑70 (MQ=255)
                            gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    >  1:2757459/1‑70 (MQ=255)
                            gtgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    >  1:2419946/1‑70 (MQ=255)
                             tgtTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATAc                                                  <  1:1588034/55‑1 (MQ=255)
                               ttGAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGATAAATACAAAAAAGACGGCACCAt                                 <  1:1927494/70‑1 (MQ=255)
                                 gAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGc                               >  1:2199687/1‑70 (MQ=255)
                                 gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAAt                                                    >  1:3733289/1‑49 (MQ=255)
                                 gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc                              >  1:114306/1‑71 (MQ=255)
                                 gAACTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc                              >  1:2829525/1‑71 (MQ=255)
                                   aCTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCt                             >  1:2616260/1‑70 (MQ=255)
                                    cTGACGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGcc                              >  1:155899/1‑68 (MQ=255)
                                       aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa                                            >  1:1359732/1‑51 (MQ=255)
                                       aCGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACaaaaaa                                            >  1:143461/1‑51 (MQ=255)
                                       aCGCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaa                          <  1:602871/69‑1 (MQ=255)
                                         gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACaaaaaa                                            >  1:1591633/1‑49 (MQ=255)
                                         gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                      >  1:2686568/1‑71 (MQ=255)
                                         gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                      >  1:457196/1‑71 (MQ=255)
                                         gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                      >  1:2507619/1‑71 (MQ=255)
                                         gCCGAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGc                      >  1:3516704/1‑71 (MQ=255)
                                          ccGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCt                     <  1:2175328/71‑1 (MQ=255)
                                            gAATATGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGa                   >  1:16642/1‑71 (MQ=255)
                                               tatGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGt                <  1:169628/71‑1 (MQ=255)
                                                 tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGtt               <  1:962460/70‑1 (MQ=255)
                                                 tGGCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTa              <  1:2248280/71‑1 (MQ=255)
                                                   gCAGGTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTaa             <  1:3676005/70‑1 (MQ=255)
                                                      ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAAcc           >  1:1770742/1‑69 (MQ=255)
                                                      ggTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCa          >  1:2413012/1‑70 (MQ=255)
                                                       gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa        <  1:2890479/71‑1 (MQ=255)
                                                       gTCCCGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCaaa        <  1:2326991/71‑1 (MQ=255)
                                                         cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGAc                  >  1:1794505/1‑59 (MQ=255)
                                                         cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg                 >  1:2261450/1‑60 (MQ=255)
                                                         cccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACg                 >  1:1722575/1‑60 (MQ=255)
                                                          ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa                        >  1:3190382/1‑52 (MQ=255)
                                                          ccGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGaaaa                        >  1:435538/1‑52 (MQ=255)
                                                          ccGCCTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATc      <  1:2360818/70‑1 (MQ=255)
                                                            gCCTTCGACGCGCTGGAGAAAAACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATacac   <  1:1675265/71‑1 (MQ=255)
                                                             ccTTCAACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCAcc  <  1:2498058/71‑1 (MQ=255)
                                                               ttCGACGCGCTGGAGAAATACAAAAAAGACGGCAc                                    <  1:558214/35‑1 (MQ=255)
                                                                  |                                                                 
GCGATGATGGATGGCGAAGCGAACGCCAGTGTTGAACTGACGCCGAATATGGCAGGTCCCGCCTTCGACGCGCTGGAGAAATACAAAAAAGACGGCACCATGCCTGAAAAGCTGACGTTAACCAAATCCACC  >  Exported/2892111‑2892242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: