Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,895,458 G→A 62.0% A164A (GCG→GCA yjfF → predicted sugar transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,895,4580GA62.0% 37.0 / 53.6 50A164A (GCG→GCAyjfFpredicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (6/13);  new base A (16/15);  total (22/28)
Fisher's exact test for biased strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGG  >  Exported/2895391‑2895523
                                                                   |                                                                 
aCCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCg                                                                   >  1:2930751/1‑68 (MQ=255)
aCCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCg                                                                   >  1:1839555/1‑68 (MQ=255)
aCCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCg                                                                   >  1:1793566/1‑68 (MQ=255)
     cccATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGAct                                                           >  1:878082/1‑71 (MQ=255)
      ccATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGActg                                                          >  1:1822284/1‑71 (MQ=255)
         tttATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGActgct                                                        >  1:175161/1‑70 (MQ=255)
                cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGActgct                                                        <  1:3296506/63‑1 (MQ=255)
                cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGActgct                                                        <  1:3733957/63‑1 (MQ=255)
                cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTg                                                 >  1:527165/1‑70 (MQ=255)
                cacGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTg                                                 >  1:1695943/1‑70 (MQ=255)
                  cGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCg                                              <  1:2946626/71‑1 (MQ=255)
                  cGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCg                                              <  1:2742580/71‑1 (MQ=255)
                  cGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCg                                              <  1:764837/71‑1 (MQ=255)
                      ctcAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGa                                                      <  1:2192372/59‑1 (MQ=255)
                       tcAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGt                                                   <  1:3058883/61‑1 (MQ=255)
                       tcAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggt                                         >  1:878246/1‑71 (MQ=255)
                       tcAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggt                                         >  1:576359/1‑71 (MQ=255)
                       tcAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtgga                                         >  1:3639953/1‑70 (MQ=255)
                         aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCggtggttg                                       >  1:1506334/1‑69 (MQ=255)
                         aaGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtgg                                          <  1:2020793/68‑1 (MQ=255)
                          aGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGc                                               >  1:462401/1‑62 (MQ=255)
                          aGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGc                                               >  1:2019677/1‑62 (MQ=255)
                          aGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggtggtggt                                      >  1:700244/1‑71 (MQ=255)
                               tGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCggttg                                          <  1:2404344/62‑3 (MQ=255)
                                 cGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCgg                                <  1:917978/70‑1 (MQ=255)
                                       aaaTCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTc                         <  1:3157220/71‑1 (MQ=255)
                                              tGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgcg                   >  1:846969/1‑70 (MQ=255)
                                              tGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTcgcg                   >  1:989968/1‑70 (MQ=255)
                                               ggcggcGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCggt                                            >  1:711878/1‑44 (MQ=255)
                                                gcggcgGTCGCTTAAGTGCGATGTGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCa                 <  1:2637508/70‑1 (MQ=255)
                                                gcggcgGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCa                 <  1:385330/70‑1 (MQ=255)
                                                gcggcgGTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCgcgc                  <  1:1780974/69‑1 (MQ=255)
                                                    cggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTa            >  1:3403903/1‑71 (MQ=255)
                                                     ggTCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCg                                 >  1:2119478/1‑49 (MQ=255)
                                                      gTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTTCTCGCGCATCGTa            <  1:981042/69‑1 (MQ=255)
                                                      gTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCa                 <  1:3431607/64‑1 (MQ=255)
                                                      gTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTAcc          <  1:1408267/71‑1 (MQ=255)
                                                       tCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCgg                                <  1:1456075/48‑1 (MQ=255)
                                                       tCGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCgg                                <  1:989347/48‑1 (MQ=255)
                                                        cGCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCg        >  1:2224287/1‑71 (MQ=255)
                                                         gCTTAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGt       <  1:3735477/71‑1 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCt                       <  1:2125944/53‑1 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCt                       <  1:881548/53‑1 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCt                       <  1:1333462/53‑1 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     >  1:3155505/1‑71 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     >  1:1061484/1‑71 (MQ=255)
                                                           ttAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGt       <  1:520482/69‑1 (MQ=255)
                                                            tAAGTGCAATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGtttt    >  1:1232320/1‑71 (MQ=255)
                                                             aaGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     <  1:527188/69‑1 (MQ=255)
                                                             aaGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     <  1:1842814/69‑1 (MQ=255)
                                                             aaGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGttt     <  1:1324860/69‑1 (MQ=255)
                                                               gTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTgg  >  1:2991634/1‑70 (MQ=255)
                                                                  cGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCAt                <  1:1238958/53‑1 (MQ=255)
                                                                   gATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGc                               <  1:399974/37‑1 (MQ=255)
                                                                   gATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGc                               <  1:2925366/37‑1 (MQ=255)
                                                                   |                                                                 
ACCATCCCATTTATGACACGCTCTCAAGCCTTGCGTGGAAAATCCCTGGCGGCGGTCGCTTAAGTGCGATGGGACTGCTGATGTTGGCGGTGGTGGTTATCGGCATATTCCTCGCGCATCGTACCCGTTTTGG  >  Exported/2895391‑2895523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: