Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 377,108 A→T 7.6% intergenic (‑22/‑89) fepD ← / → ybdA iron‑enterobactin transporter subunit/predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported377,1080AT 7.6% 191.0 / 6.6 66intergenic (‑22/‑89)fepD/ybdAiron‑enterobactin transporter subunit/predicted transporter
Reads supporting (aligned to +/- strand):  ref base A (27/34);  new base T (2/3);  total (29/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATA  >  Exported/377039‑377176
                                                                     |                                                                    
cAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGaaa                                                                      >  1:1511250/1‑70 (MQ=255)
cAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaat                                                                     >  1:322500/1‑71 (MQ=255)
 aaTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaatt                                                                    <  1:2637753/71‑1 (MQ=255)
 aaTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaatt                                                                    <  1:507188/71‑1 (MQ=255)
 aaTCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaatt                                                                    <  1:1825287/71‑1 (MQ=255)
     cGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaattaat                                                                 >  1:1942898/1‑70 (MQ=255)
     cGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAaattaat                                                                 >  1:2157397/1‑70 (MQ=255)
      gggCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAattaatta                                                               <  1:86053/71‑1 (MQ=255)
       ggCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttat                                                              >  1:260524/1‑71 (MQ=255)
        gCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatc                                                             >  1:2714233/1‑71 (MQ=255)
          aCGGCAATGGCGCGTGTCACGGCAACAGAACCAGTCATACGAACTTCCATGATAATGAAATTAAttatcgt                                                           >  1:3112033/1‑71 (MQ=255)
              cAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAattaatta                                                               >  1:2334295/1‑63 (MQ=255)
               aaTGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                      <  1:1020459/71‑1 (MQ=255)
               aaTGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                      <  1:407331/71‑1 (MQ=255)
                 tGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                    <  1:3363074/71‑1 (MQ=255)
                  ggCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATc                                                   >  1:1962610/1‑71 (MQ=255)
                  ggCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATc                                                   >  1:1958223/1‑71 (MQ=255)
                   gcgcGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcaga                                                    >  1:2840828/1‑66 (MQ=255)
                     gcgTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTa                                                <  1:1282427/71‑1 (MQ=255)
                      cgTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTAt                                               <  1:71599/71‑1 (MQ=255)
                          tCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTgg                                           <  1:1618884/71‑1 (MQ=255)
                            aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                    <  1:2282973/60‑1 (MQ=255)
                            aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGAt                                                    <  1:2068965/60‑1 (MQ=255)
                            aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTAt                                               <  1:2735353/65‑1 (MQ=255)
                            aCGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          >  1:3170685/1‑70 (MQ=255)
                                cAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     <  1:535955/71‑1 (MQ=255)
                                 aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGtt                                    <  1:3209827/71‑1 (MQ=255)
                                 aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:3224774/1‑70 (MQ=255)
                                 aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:288609/1‑70 (MQ=255)
                                 aaCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:1157318/1‑70 (MQ=255)
                                  aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:1575665/1‑69 (MQ=255)
                                  aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:1536296/1‑69 (MQ=255)
                                  aCAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGt                                     >  1:3542214/1‑69 (MQ=255)
                                   cAGAACCAGACATACGAACTTCCATGATAATGAAATTAAttatcgt                                                           <  1:106851/46‑1 (MQ=255)
                                    aGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGc                                 <  1:2761244/71‑1 (MQ=255)
                                    aGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGc                                 <  1:3246406/71‑1 (MQ=255)
                                     gAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCa                                <  1:101615/71‑1 (MQ=255)
                                     gAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCa                                <  1:3551172/71‑1 (MQ=255)
                                      aaCCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                               >  1:750521/1‑71 (MQ=255)
                                      aaCCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                               >  1:3077840/1‑71 (MQ=255)
                                        ccAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                      <  1:510616/46‑1 (MQ=255)
                                        ccAGACATACGAACTTCCATGATAATGAAATTAAttatcgttatcg                                                      <  1:2240170/46‑1 (MQ=255)
                                           gACATACGAACTTCCATGATAATGAAATTAATTATAGTTATCGATCTTATTTGGATATGTTAGCAtgtg                            >  1:1581571/1‑69 (MQ=255)
                                            aCATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          <  1:2695204/54‑1 (MQ=255)
                                            aCATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          <  1:312530/54‑1 (MQ=255)
                                            aCATACGAACTTCCATGATAATGAAATTAATTATCGTTTCGATCTTATTTGGa                                          <  1:314989/53‑1 (MQ=255)
                                                aCGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCtt                                                 <  1:2304308/43‑1 (MQ=255)
                                                aCGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCtt                                                 <  1:117190/43‑1 (MQ=255)
                                                aCGAACTTCCATGACAATGAAATTAATTATCGTTATCGATCtt                                                 <  1:1981079/43‑1 (MQ=255)
                                                 cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          <  1:1079328/49‑1 (MQ=255)
                                                 cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          <  1:1029691/49‑1 (MQ=255)
                                                 cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGa                                          <  1:255102/49‑1 (MQ=255)
                                                 cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                      >  1:3660957/1‑69 (MQ=255)
                                                 cGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                      >  1:1027629/1‑69 (MQ=255)
                                                  gAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                      >  1:3152176/1‑68 (MQ=255)
                                                     cTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAAt                <  1:2137943/71‑1 (MQ=255)
                                                       tCCATGATATTGATATTAATTATCGTTATCGATCtt                                                 <  1:2638157/36‑1 (MQ=37)
                                                        ccATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATa               <  1:1922845/69‑1 (MQ=255)
                                                        ccATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAgg             >  1:3277673/1‑71 (MQ=255)
                                                         cATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAgg             <  1:3021942/70‑1 (MQ=255)
                                                         aacgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCt                      <  1:2679491/58‑1 (MQ=37)
                                                          acgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                               >  1:977289/3‑51 (MQ=25)
                                                          acgATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATGTTAGCAt                               >  1:1251318/3‑51 (MQ=25)
                                                           tGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt          >  1:3149905/1‑71 (MQ=255)
                                                           tGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt          >  1:1915890/1‑71 (MQ=255)
                                                            gATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATg                                      <  1:1607141/42‑1 (MQ=25)
                                                            gATAATTAATTTCATTATCGTTATCGATCTTATTTGGATATg                                      <  1:643583/42‑1 (MQ=25)
                                                            gATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAtt         >  1:986834/1‑71 (MQ=255)
                                                            gATAATGAAATTAATTATCGTTATCGATATTATTTGGATATGTTAGc                                 >  1:1957861/1‑47 (MQ=255)
                                                              taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                    >  1:144464/1‑42 (MQ=25)
                                                              taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                    >  1:2008541/1‑42 (MQ=25)
                                                              taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                    >  1:1089039/1‑42 (MQ=25)
                                                              taattaatTTCATTATCGTTATCGATCTTATTTGGATATGtt                                    >  1:255457/1‑42 (MQ=25)
                                                              tAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTa       <  1:534874/71‑1 (MQ=255)
                                                              tAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTa       <  1:818938/71‑1 (MQ=255)
                                                              tAATGAAATTAATTATAGTTATCGATCTTATTTGGATATGTTAGCAt                               >  1:3628334/1‑47 (MQ=255)
                                                                aTGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAATAATAGGTATTTaa      <  1:2398993/70‑1 (MQ=255)
                                                                 tGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt          >  1:764446/1‑65 (MQ=255)
                                                                 tGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTAt          >  1:801841/1‑65 (MQ=255)
                                                                     attaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAata  <  1:3717363/69‑1 (MQ=255)
                                                                     attaattaTCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAata  <  1:1289861/69‑1 (MQ=255)
                                                                     |                                                                    
CAATCCGGGCACGGCAATGGCGCGTGTCACGGCAACAGAACCAGACATACGAACTTCCATGATAATGAAATTAATTATCGTTATCGATCTTATTTGGATATGTTAGCATGTGCAGCCTAAGAATAGGTATTTAAAATA  >  Exported/377039‑377176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: