Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 40,322 C→T 5.7% P81L (CCG→CTG)  fixA → predicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported40,3220CT 5.7% 339.4 / 6.7 104P81L (CCG→CTG) fixApredicted electron transfer flavoprotein subunit, ETFP adenine nucleotide‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (46/52);  new base T (3/3);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 110 total aligned reads displayed.

ACAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT  >  Exported/40252‑40391
                                                                      |                                                                     
aCAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGccc                                                                       >  1:2795670/1‑71 (MQ=255)
 cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg                                                                      <  1:1117365/71‑1 (MQ=255)
 cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg                                                                      >  1:1659519/1‑71 (MQ=255)
 cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg                                                                      >  1:416671/1‑71 (MQ=255)
 cAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg                                                                      >  1:174239/1‑71 (MQ=255)
 cAGCCTTAAGTGGGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCg                                                                      <  1:682260/71‑1 (MQ=255)
   gCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa                                                                    <  1:597107/71‑1 (MQ=255)
    ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt                                                                   >  1:2736683/1‑71 (MQ=255)
    ccTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt                                                                   <  1:1418417/71‑1 (MQ=255)
       tAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGaa                                                                >  1:2736496/1‑71 (MQ=255)
        aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa                                                                    >  1:1915158/1‑66 (MQ=255)
        aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGa                                                                 <  1:165923/69‑1 (MQ=255)
        aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGa                                                                 <  1:2442613/69‑1 (MQ=255)
        aaGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAAc                                                               >  1:2301033/1‑71 (MQ=255)
         aGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACt                                                              <  1:2134842/71‑1 (MQ=255)
          gtgtGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACt                                                              >  1:2177700/1‑70 (MQ=255)
           tgtgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGa                                                            <  1:1798842/71‑1 (MQ=255)
             tgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGAtt                                                          >  1:487716/1‑71 (MQ=255)
             tgGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGAtt                                                          >  1:3544786/1‑71 (MQ=255)
               ggCGGTAAAGCCCTTACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg                                                         <  1:617844/70‑1 (MQ=255)
               ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt                                                        <  1:3663493/71‑1 (MQ=255)
               ggCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg                                                         <  1:2337582/70‑1 (MQ=255)
                gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt                                                           <  1:1204609/67‑1 (MQ=255)
                gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt                                                           <  1:914451/67‑1 (MQ=255)
                gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGAt                                                           <  1:3327001/67‑1 (MQ=255)
                gCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg                                                       >  1:3593964/1‑71 (MQ=255)
                    tAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAattgattg                                                         <  1:8051/65‑1 (MQ=255)
                    tAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg                                                         <  1:2148054/65‑1 (MQ=255)
                     aaaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat                                                  >  1:1443036/1‑71 (MQ=255)
                      aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt                                                 >  1:289964/1‑71 (MQ=255)
                      aaGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat                                                  <  1:1823803/70‑1 (MQ=255)
                       aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGa                                                                    <  1:181808/51‑1 (MQ=255)
                       aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattg                                                <  1:3545868/71‑1 (MQ=255)
                       aGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgatt                                                 >  1:2130979/1‑70 (MQ=255)
                        gCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattga                                               <  1:1116821/71‑1 (MQ=255)
                            tGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat                                              <  1:1704763/68‑1 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg                                                      >  1:3041212/1‑59 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg                                                      >  1:1032475/1‑59 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCTGGATGAACTGATTgtgg                                                      >  1:1161172/1‑59 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGAt                                                                   >  1:3105361/1‑46 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc                                           >  1:3620101/1‑70 (MQ=255)
                             gACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc                                           >  1:1741470/1‑70 (MQ=255)
                               ccAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGAc                                           <  1:1449023/68‑1 (MQ=255)
                                  aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg                                                       <  1:1044255/53‑1 (MQ=255)
                                  aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg                                                       <  1:3735032/53‑1 (MQ=255)
                                  aCGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgtg                                                       <  1:3060515/53‑1 (MQ=255)
                                   cGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc                                     >  1:3196553/1‑70 (MQ=255)
                                    gCCAAAGGGCGTGAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc                                     >  1:728921/1‑69 (MQ=255)
                                    gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTgtggt                                                     >  1:464765/1‑53 (MQ=255)
                                    gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc                                     >  1:2531707/1‑69 (MQ=255)
                                    gCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc                                     >  1:903135/1‑69 (MQ=255)
                                     ccAAAGGGCGTAAAGATGTTCTATCGCGCGGCCCGGATGAACTGATtg                                                         >  1:2461243/1‑48 (MQ=255)
                                     ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg                                                         >  1:2725510/1‑48 (MQ=255)
                                     ccAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtg                                                         >  1:2853047/1‑48 (MQ=255)
                                      cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATtgatga                                            >  1:1491540/1‑60 (MQ=255)
                                      cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATtgatga                                            >  1:2944526/1‑60 (MQ=255)
                                      cAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg                                  >  1:2238661/1‑70 (MQ=255)
                                       aaaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGtt                                      >  1:3566322/1‑65 (MQ=255)
                                       aaaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGtt                                      >  1:3581954/1‑65 (MQ=255)
                                        aaGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATtgt                                                        <  1:923493/46‑1 (MQ=255)
                                         aGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAgg                              <  1:3677618/71‑1 (MQ=255)
                                          gggCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGc                             <  1:3643301/71‑1 (MQ=255)
                                          gggCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGc                             <  1:1214239/71‑1 (MQ=255)
                                             cGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACt                          <  1:2240839/71‑1 (MQ=255)
                                             cGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACt                          <  1:386949/71‑1 (MQ=255)
                                               tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTgtggt                                                     <  1:1164383/42‑1 (MQ=255)
                                               tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc                        <  1:2948967/71‑1 (MQ=255)
                                               tAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGc                        <  1:3096832/71‑1 (MQ=255)
                                               tAAAGATGTGCTATCGCGCGGCACGGATGAACTGATTgtggt                                                     <  1:845930/42‑1 (MQ=255)
                                                aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg                                  <  1:247612/60‑1 (MQ=255)
                                                aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAg                                  <  1:3057863/60‑1 (MQ=255)
                                                aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCa                                <  1:3308049/62‑1 (MQ=255)
                                                aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGcc                       >  1:1258197/1‑71 (MQ=255)
                                                aaaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGcc                       >  1:1518105/1‑71 (MQ=255)
                                                 aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat                                              >  1:773324/1‑47 (MQ=255)
                                                 aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat                                              >  1:146351/1‑47 (MQ=255)
                                                 aaGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgattgat                                              >  1:2054567/1‑47 (MQ=255)
                                                  aGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCg                      <  1:3626871/70‑1 (MQ=255)
                                                   gATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGtgat                                                  <  1:185235/41‑1 (MQ=255)
                                                   gATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGca                    <  1:2317170/71‑1 (MQ=255)
                                                    aTGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGt                                       <  1:2611095/51‑1 (MQ=255)
                                                     tgtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGcaa                   >  1:476312/1‑70 (MQ=255)
                                                      gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCa                                         <  1:2401416/47‑1 (MQ=255)
                                                      gtgCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCa                                         <  1:3345284/47‑1 (MQ=255)
                                                         cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTc                                     <  1:1308624/48‑1 (MQ=255)
                                                         cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAAc              >  1:1002405/1‑71 (MQ=255)
                                                         cTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAAc              <  1:3001104/71‑1 (MQ=255)
                                                          tATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCa                                <  1:3312378/52‑1 (MQ=255)
                                                            tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACgg            <  1:3525639/70‑1 (MQ=255)
                                                            tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGc           <  1:2313260/71‑1 (MQ=255)
                                                            tCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGc           <  1:1667585/71‑1 (MQ=255)
                                                              gcgcgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGc                                 <  1:1790498/47‑1 (MQ=255)
                                                                 cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg      <  1:1317377/71‑1 (MQ=255)
                                                                 cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg      <  1:2758419/71‑1 (MQ=255)
                                                                 cgGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAgcg      <  1:2205278/71‑1 (MQ=255)
                                                                    cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGa         <  1:3490668/65‑1 (MQ=255)
                                                                    cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc   <  1:3495318/71‑1 (MQ=255)
                                                                    cccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCAc   <  1:2403885/71‑1 (MQ=255)
                                                                     ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt  >  1:1415494/1‑71 (MQ=255)
                                                                     ccGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACt  <  1:1644501/71‑1 (MQ=255)
                                                                      |                                                                     
ACAGCCTTAAGTGTGGGCGGTAAAGCCCTGACCAACGCCAAAGGGCGTAAAGATGTGCTATCGCGCGGCCCGGATGAACTGATTGTGGTGATTGATGACCAGTTCGAGCAGGCACTGCCGCAACAAACGGCGAGCGCACT  >  Exported/40252‑40391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: