| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 418,094 | C→T | 9.9% | A55T (GCT→ACT) | miaB ← | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 418,094 | 0 | C | T | 9.9% | 301.2 / 18.4 | 101 | A55T (GCT→ACT) | miaB | isopentenyl‑adenosine A37 tRNA methylthiolase |
| Reads supporting (aligned to +/- strand): ref base C (47/44); new base T (3/7); total (50/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
AATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > Exported/418026‑418162 | aaTCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCt > 1:12828/1‑71 (MQ=255) gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:2403995/1‑71 (MQ=255) gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:2793722/1‑71 (MQ=255) gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:1396872/1‑71 (MQ=255) tCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:3432270/71‑1 (MQ=255) ggATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGa > 1:439746/1‑70 (MQ=255) gATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAt < 1:884611/70‑1 (MQ=255) aTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGa < 1:3620661/68‑1 (MQ=255) aTTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGAtt > 1:2599418/1‑70 (MQ=255) cttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:3308206/1‑70 (MQ=255) cttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAg > 1:2402315/1‑70 (MQ=255) tctcTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAg > 1:1313059/1‑71 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:357231/1‑59 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:2310581/68‑1 (MQ=255) ctctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:2319047/68‑1 (MQ=255) tctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCgg < 1:2372195/60‑1 (MQ=255) tctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCgg < 1:2546245/60‑1 (MQ=255) tctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGgt > 1:2861345/1‑71 (MQ=255) ctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGgtg > 1:2657404/1‑71 (MQ=255) ctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:766897/1‑57 (MQ=255) ctTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgc > 1:1719597/1‑57 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGgt < 1:1429114/69‑1 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGgt < 1:1491595/69‑1 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:420873/57‑1 (MQ=255) ttttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:224128/57‑1 (MQ=255) tttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCGTTCTCGCGGATTGa < 1:3291204/62‑1 (MQ=255) tttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCGTTCTCGCGGATTGa < 1:3295551/62‑1 (MQ=255) tttAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTcgcg < 1:540034/56‑1 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGtt > 1:443955/1‑69 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGtt > 1:2894668/1‑69 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:197511/70‑1 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:2960582/70‑1 (MQ=255) tAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTc < 1:2938681/70‑1 (MQ=255) aaCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:3335641/1‑44 (MQ=255) aaCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:492980/1‑44 (MQ=255) aaCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGc > 1:404211/1‑44 (MQ=255) aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagca < 1:1447232/70‑1 (MQ=255) aGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagca < 1:356844/70‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcagc < 1:3126243/71‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcagc < 1:685679/71‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTcagcagc < 1:1368529/71‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTg < 1:1826468/54‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTg < 1:1840150/54‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag < 1:1741085/70‑1 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:838671/1‑70 (MQ=255) gTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcag > 1:450198/1‑70 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagc > 1:1868421/1‑70 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagc > 1:1315627/1‑70 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagc < 1:1209017/70‑1 (MQ=255) tttCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagcagc > 1:1131725/1‑70 (MQ=255) cAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAgcagcagca < 1:2352839/70‑1 (MQ=255) aGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGcaca < 1:1444836/71‑1 (MQ=255) aGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGcaca < 1:3266652/71‑1 (MQ=255) cGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc > 1:2261233/1‑71 (MQ=255) aCCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCg > 1:2593271/1‑71 (MQ=255) aCCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCg > 1:11539/1‑71 (MQ=255) aCCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCg > 1:1477219/1‑71 (MQ=255) aCCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCg > 1:770159/1‑71 (MQ=255) ccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCTGATTGAGc < 1:3633881/45‑1 (MQ=255) ccAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGc < 1:2212081/45‑1 (MQ=255) aCTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttct > 1:2787613/1‑71 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagca > 1:2989530/1‑53 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc > 1:2073143/1‑63 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATc > 1:2068879/1‑63 (MQ=255) cTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt > 1:3160182/1‑71 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:3138310/51‑1 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:2511014/51‑1 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:1110747/51‑1 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagc < 1:28879/51‑1 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCttcttc > 1:2959277/1‑71 (MQ=255) tGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCttcttc > 1:2150759/1‑71 (MQ=255) gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGcttctt > 1:1361378/1‑69 (MQ=255) gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:466098/1‑71 (MQ=255) gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:2417376/1‑71 (MQ=255) gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTcc > 1:2344898/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:642415/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:1144338/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:226812/1‑71 (MQ=255) aTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCg > 1:3188649/1‑71 (MQ=255) tGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGc < 1:78820/71‑1 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTcagca > 1:2149138/1‑45 (MQ=255) aGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACg < 1:658404/71‑1 (MQ=255) gACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:2137247/71‑1 (MQ=255) gACTTTTTCCTGAACCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGt < 1:1000852/71‑1 (MQ=255) tttttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCgg > 1:3665689/1‑71 (MQ=255) tttttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCgg > 1:2018086/1‑71 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCAc < 1:1276247/65‑1 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCAc < 1:1681518/65‑1 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCAc < 1:2054500/65‑1 (MQ=255) ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:901397/1‑71 (MQ=255) tttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGt > 1:2841955/1‑70 (MQ=255) ttCCTGAGTCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTc < 1:1644577/70‑1 (MQ=255) ttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTc < 1:3531999/70‑1 (MQ=255) tCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCa < 1:2255555/70‑1 (MQ=255) cTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGtt < 1:1606157/71‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg < 1:3553818/69‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg < 1:1322408/69‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTg < 1:1276511/69‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAt < 1:541193/71‑1 (MQ=255) aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAt < 1:1027319/71‑1 (MQ=255) | AATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGAT > Exported/418026‑418162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |