BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	421,280	G→A	46.2%	A400V (GCA→GTA)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	421,280	0	G	A	46.2%	26.5 / 144.0	104	A400V (GCA→GTA)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand): ref base G (29/27); new base A (33/15); total (62/42)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 106 total aligned reads displayed.

CTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTT  >  Exported/421222‑421349

cTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGccc                                                            >  1:2788118/1‑70 (MQ=255)

  gtgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAg                                                         >  1:1879672/1‑71 (MQ=255)

   tgGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCa                                                          <  1:664489/69‑1 (MQ=255)

       ttAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGa                                                    <  1:3428392/71‑1 (MQ=255)

       ttAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGa                                                    <  1:116005/71‑1 (MQ=255)

        tAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGa                                                    >  1:1731538/1‑70 (MQ=255)

        tAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGa                                                    >  1:2979416/1‑70 (MQ=255)

           tACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATc                                                <  1:3609699/71‑1 (MQ=255)

           tACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATc                                                >  1:2290845/1‑71 (MQ=255)

           tACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATc                                                >  1:948602/1‑71 (MQ=255)

           tACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATc                                                >  1:175965/1‑71 (MQ=255)

            aCGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCg                                               >  1:2910979/1‑71 (MQ=255)

            aCGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCg                                               >  1:160467/1‑71 (MQ=255)

              gCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCt                                             <  1:1077441/71‑1 (MQ=255)

              gCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCAACGCCCCAGGCTGACATCg                                               >  1:1776842/1‑69 (MQ=255)

               cATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCa                                                                 >  1:2269548/1‑50 (MQ=255)

                aTCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCtt                                            <  1:934170/70‑1 (MQ=255)

                 tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGccc                                                            <  1:1845128/53‑1 (MQ=255)

                 tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGccc                                                            <  1:140538/53‑1 (MQ=255)

                 tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                          <  1:2637410/71‑1 (MQ=255)

                 tCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttg                                          <  1:445412/71‑1 (MQ=255)

                  cGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTc                                                                   >  1:1599224/1‑45 (MQ=255)

                  cGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTc                                                                   >  1:3380627/1‑45 (MQ=255)

                  cGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgt                                         >  1:3656716/1‑71 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTg                                                     >  1:2820681/1‑58 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTg                                                     >  1:430554/1‑58 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTg                                                     >  1:207858/1‑58 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:408815/71‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgtt                                        >  1:1254276/1‑71 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgt                                         <  1:1650116/70‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgt                                         <  1:635941/70‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgt                                         <  1:2205652/70‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:1043625/71‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:3501899/71‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:3655595/71‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:2376021/71‑1 (MQ=255)

                   gCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTttgtt                                        <  1:90898/71‑1 (MQ=255)

                    ccACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCt                                                      <  1:1205872/56‑1 (MQ=255)

                    ccACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTttgttg                                       <  1:3568065/71‑1 (MQ=255)

                     cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTcc                                                                  >  1:2137929/1‑43 (MQ=255)

                     cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTcc                                                                  >  1:2116676/1‑43 (MQ=255)

                     cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTcc                                                                  >  1:2226644/1‑43 (MQ=255)

                     cacaTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTcc                                                                  >  1:2286583/1‑43 (MQ=255)

                       caTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCt                                                      >  1:1038832/1‑53 (MQ=255)

                       caTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCt                                                      >  1:372281/1‑53 (MQ=255)

                        aTCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCt                                                      <  1:3638178/52‑1 (MQ=255)

                         tcgaGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcg                                  >  1:1597401/4‑71 (MQ=255)

                         tCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCttt                                           >  1:1357737/1‑62 (MQ=255)

                         tCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCttt                                           >  1:1062998/1‑62 (MQ=255)

                         tCAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcg                                  >  1:659951/1‑71 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCa                                                                 >  1:3259455/1‑39 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCa                                                                 >  1:1769223/1‑39 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGa                                                    >  1:3493776/1‑52 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGa                                                    >  1:1891551/1‑52 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGa                                                    >  1:313468/1‑52 (MQ=255)

                          cAAGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcg                                  >  1:752424/1‑70 (MQ=255)

                          cAAGGAATTATTTGTCGAGGAACGGAACGCGTGCTTCCa                                                                 >  1:2898011/1‑39 (MQ=255)

                           aaGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCAGGCCCCAGGCTGACa                                                  >  1:3613012/1‑53 (MQ=255)

                           aaGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGc                                                              >  1:3414805/1‑41 (MQ=255)

                           aaGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcg                                >  1:761432/1‑71 (MQ=255)

                           aaGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcg                                >  1:899817/1‑71 (MQ=255)

                           aaGGAATTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCg                                               >  1:1647148/1‑56 (MQ=255)

                             ggAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcgcg                              <  1:204882/71‑1 (MQ=255)

                               aaTTTTTTGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCAcgcgcg                              >  1:2868538/1‑69 (MQ=255)

                                aTTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAg                           >  1:1295115/1‑71 (MQ=255)

                                 ttttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt                          >  1:2906139/1‑71 (MQ=255)

                                 ttttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt                          >  1:1837687/1‑71 (MQ=255)

                                 ttttttGTCGAGGAACGGAACGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGt                          >  1:2731598/1‑71 (MQ=255)

                                     ttGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcat                       <  1:2410225/70‑1 (MQ=255)

                                        tCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTcatacat                   <  1:707648/71‑1 (MQ=255)

                                          gAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACatat                 <  1:1393503/71‑1 (MQ=255)

                                          gAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACatat                 <  1:2908279/71‑1 (MQ=255)

                                            ggaacggaacGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg                >  1:2179720/1‑70 (MQ=255)

                                            ggaacggaacGCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg                >  1:1892842/1‑70 (MQ=255)

                                             gaacggaacgCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCa              <  1:2633526/71‑1 (MQ=255)

                                             gaacggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATg                >  1:3468660/1‑69 (MQ=255)

                                              aacggaacgCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAg             <  1:1353985/71‑1 (MQ=255)

                                               acggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAgg            <  1:928597/71‑1 (MQ=255)

                                               acggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAgg            <  1:1856974/71‑1 (MQ=255)

                                               acggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAgg            <  1:2486527/71‑1 (MQ=255)

                                                 ggaacgCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc          <  1:3276716/71‑1 (MQ=255)

                                                 ggaacgCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc          <  1:2647574/71‑1 (MQ=255)

                                                 ggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc          >  1:2349240/1‑71 (MQ=255)

                                                 ggaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGc          >  1:3053919/1‑71 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGc                                     >  1:181983/1‑43 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCa                                    >  1:1515292/1‑44 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCa                                    >  1:1335216/1‑44 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCa                                    >  1:515672/1‑44 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCa                                    >  1:522719/1‑44 (MQ=255)

                                                  gaacgCGTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCa                                    >  1:1135023/1‑44 (MQ=255)

                                                      gcgTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCcag       >  1:3582998/1‑69 (MQ=255)

                                                      gcgTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCcag       >  1:3179972/1‑69 (MQ=255)

                                                       cgTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCcagcag    <  1:856074/71‑1 (MQ=255)

                                                       cgTACTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCcagcag    <  1:1292530/71‑1 (MQ=255)

                                                         tGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGGAGtt  <  1:228232/71‑1 (MQ=255)

                                                         tGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGtt  <  1:3157849/71‑1 (MQ=255)

                                                         tGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGtt  <  1:1999954/71‑1 (MQ=255)

                                                         tGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGtt  <  1:1899333/71‑1 (MQ=255)

                                                          gCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCCCGCGCGCAGTCATACATATGCAGGGCCAGCAGtt  >  1:3051766/1‑70 (MQ=255)

                                                          gCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGtt  >  1:1050223/1‑70 (MQ=255)

CTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTGCTTCCACGCCCCAGGCTGACATCGCTTTGTTGGCACGCGCGCAGTCATACATATGCAGGGCCAGCAGTT  >  Exported/421222‑421349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`