Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 450,341 C→G 5.4% intergenic (‑4/+19) kdpB ← / ← kdpA potassium translocating ATPase, subunit B/potassium translocating ATPase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported450,3410CG 5.4% 234.0 / 2.0 74intergenic (‑4/+19)kdpB/kdpApotassium translocating ATPase, subunit B/potassium translocating ATPase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (33/37);  new base G (2/2);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

CACCGCTTCTTTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATAAAGGT  >  Exported/450272‑450410
                                                                     |                                                                     
cACCGCTTCTTTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTc                                                                       <  1:655153/70‑1 (MQ=255)
     cttcttTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGct                                                                 >  1:2381285/1‑71 (MQ=255)
        cttTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGGGCCAGTTGTTTACGACTCATATTCAGTGctca                                                               <  1:1872063/70‑1 (MQ=255)
        cttTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctca                                                               <  1:2094701/70‑1 (MQ=255)
           tCAGCGCCTGAACTACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:1643406/68‑1 (MQ=255)
           tCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:3280637/68‑1 (MQ=255)
             aGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCaa                                                          >  1:171247/1‑70 (MQ=255)
              gcgcCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTGAGTGagcactga                                                           <  1:3289959/68‑3 (MQ=255)
              gcgcCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTGAGTGagcactga                                                           <  1:2246258/68‑3 (MQ=255)
                gcCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTGAGTGAGCACTGAat                                                         >  1:597038/1‑68 (MQ=38)
                gcCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAatat                                                       >  1:365797/1‑70 (MQ=255)
                gcCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGATGTTTACGACTCATATAGAGTGAGCACTGAat                                                         >  1:2409394/1‑68 (MQ=255)
                  cTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATatcat                                                    <  1:2564911/71‑1 (MQ=255)
                   tGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATatcat                                                    >  1:716749/1‑70 (MQ=255)
                     aaCGACAAGTGTTTGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATatcatc                                                   <  1:1009670/69‑1 (MQ=255)
                     aaCGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAg                                                                     >  1:1532420/1‑51 (MQ=255)
                     aaCGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAg                                                 >  1:853560/1‑71 (MQ=255)
                     aaCGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAg                                                 <  1:238476/71‑1 (MQ=255)
                           aaGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGgagaga                                           <  1:3277218/71‑1 (MQ=255)
                           aaGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:2513470/1‑67 (MQ=255)
                             gtgtTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAgg                                                >  1:2252634/1‑64 (MQ=255)
                             gtgtTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGata                                         >  1:3479911/1‑71 (MQ=255)
                                ttGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATAtt                                       <  1:2120346/70‑1 (MQ=255)
                                ttGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTc                                      >  1:2409131/1‑71 (MQ=255)
                                 tGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:2906608/46‑1 (MQ=255)
                                 tGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:1162873/46‑1 (MQ=255)
                                 tGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:856841/46‑1 (MQ=255)
                                 tGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcac                                                              <  1:1195336/46‑1 (MQ=255)
                                  ggTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAg                                                                     >  1:1277007/1‑38 (MQ=255)
                                  ggTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAg                                                                     >  1:813561/1‑38 (MQ=255)
                                  ggTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAg                                                                     >  1:936223/1‑38 (MQ=255)
                                  ggTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAg                                                                     >  1:2011643/1‑38 (MQ=255)
                                   gTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGat                                          <  1:2277185/64‑1 (MQ=255)
                                   gTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGc                                   <  1:3220880/71‑1 (MQ=255)
                                   gTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGc                                   <  1:2041498/71‑1 (MQ=255)
                                   gTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGc                                   <  1:492694/71‑1 (MQ=255)
                                    ttCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGctcact                                                             >  1:524635/1‑44 (MQ=255)
                                    ttCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGcc                                  <  1:1619492/71‑1 (MQ=255)
                                    ttCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGcc                                  <  1:2225873/71‑1 (MQ=255)
                                      cGAATAGCGCCAGTTGTTTACGACTCATATTCAGTg                                                                   >  1:2410931/1‑36 (MQ=255)
                                           aGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               <  1:8895/51‑1 (MQ=255)
                                                 aGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAgcg                     >  1:3729623/1‑71 (MQ=255)
                                                   ttgttTACGACTCATATTCAGTTCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCaa                        <  1:275120/66‑1 (MQ=255)
                                                   ttgttTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCaa                        <  1:1689561/66‑1 (MQ=255)
                                                   ttgttTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCaa                        <  1:3684108/66‑1 (MQ=255)
                                                   ttgttTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCaa                        <  1:3322091/66‑1 (MQ=255)
                                                   ttgttTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGcc                   <  1:1865134/71‑1 (MQ=255)
                                                    tgttTACGACTCATATTCAGTGCTCACTCAATATCATCAg                                                 <  1:1287895/40‑1 (MQ=255)
                                                     gttAACGACTCATATTCAGTCCTCAATCAATATCATCAGTAGAGATATTCCGcc                                  >  1:1770779/1‑54 (MQ=255)
                                                      tttACGACTCATATTCAGTGCTCACTCAATATCATCAg                                                 <  1:2180387/38‑1 (MQ=255)
                                                      tttACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGcc                                  <  1:3141704/53‑1 (MQ=255)
                                                      tttACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCcagg                <  1:1628023/71‑1 (MQ=255)
                                                       ttACGACTCATATTCAGTGCTCACTCAATATCATCAGgagaga                                           >  1:2630190/1‑43 (MQ=255)
                                                       ttACGACTCATATTCAGTGCTCACTCAATATCATCAGgagaga                                           >  1:2322055/1‑43 (MQ=255)
                                                       ttACGACTCATATTCAGTGCTCACTCAATATCATCAGgagaga                                           >  1:3488585/1‑43 (MQ=255)
                                                       ttACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGAcc                          <  1:2261609/60‑1 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:261542/1‑38 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:1378517/1‑38 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:3092387/1‑38 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:1484659/1‑38 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGga                                               >  1:1680054/1‑38 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGc                                   <  1:384319/50‑1 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCAc                                >  1:3105376/1‑53 (MQ=255)
                                                        tACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCAc                                >  1:637096/1‑53 (MQ=255)
                                                         aCGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGc                                   <  1:1551154/49‑1 (MQ=255)
                                                         aCGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCcagggc              <  1:597946/70‑1 (MQ=255)
                                                                atatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCcagg                <  1:1145357/61‑1 (MQ=255)
                                                                atatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCcagg                <  1:192800/61‑1 (MQ=255)
                                                                atatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATaa      <  1:830769/71‑1 (MQ=255)
                                                                atatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATa       <  1:3152882/70‑1 (MQ=255)
                                                                 tatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATa       >  1:620542/1‑69 (MQ=255)
                                                                 tatTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATa       >  1:1805540/1‑69 (MQ=255)
                                                                  atTCAGTGCTCACTCAATATCATCAGTAGAGATATTCCGCCACCGGACCAAgc                      >  1:1988743/1‑53 (MQ=255)
                                                                  atTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAgc                      >  1:959177/1‑53 (MQ=255)
                                                                    tCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATAAAGGt  >  1:171741/1‑71 (MQ=255)
                                                                     cAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATAAAGGt  <  1:963696/70‑1 (MQ=255)
                                                                     |                                                                     
CACCGCTTCTTTCAGCGCCTGAACGACAAGTGTTGGTTCGAATAGCGCCAGTTGTTTACGACTCATATTCAGTGCTCACTCAATATCATCAGGAGAGATATTCCGCCACCGGACCAAGCGCCAGGGCAGGGATAAAGGT  >  Exported/450272‑450410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: