| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 552,454 | C→T | 7.5% | G357D (GGT→GAT) ‡ | ybiW ← | predicted pyruvate formate lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 552,454 | 0 | C | T | 7.5% | 251.5 / 8.4 | 80 | G357D (GGT→GAT) ‡ | ybiW | predicted pyruvate formate lyase |
| Reads supporting (aligned to +/- strand): ref base C (34/40); new base T (3/3); total (37/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GAACGCAGGCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCTTTTGA > Exported/552388‑552521 | gAACGCAGGCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATc < 1:710261/71‑1 (MQ=255) aCGCAGGCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCaa < 1:1068206/71‑1 (MQ=255) aCGCAGGCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCaa > 1:883035/1‑71 (MQ=255) aGGCGACCGCAGGATTCGAGGATCGCGAAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAAc > 1:1881873/1‑68 (MQ=255) ggCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGa < 1:812876/71‑1 (MQ=255) gCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATc < 1:1539809/63‑1 (MQ=255) gCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGAt > 1:2429984/1‑71 (MQ=255) aCCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACGGCGTCCATTGGTTGATGGTCAACCaa > 1:923310/1‑65 (MQ=255) aCCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGATGGTCAACCaa > 1:2356208/1‑65 (MQ=255) aCCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGATGGTCAACCaa > 1:2235541/1‑65 (MQ=255) cGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGc < 1:2208169/71‑1 (MQ=255) cAGGATTCGTGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGAtttt < 1:3062836/67‑1 (MQ=255) cAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGc > 1:2595029/1‑69 (MQ=255) aGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAAc < 1:1045340/58‑1 (MQ=255) ggATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGA‑TTTGCCCGcc < 1:1097586/71‑1 (MQ=255) aTTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGAtt < 1:96259/61‑1 (MQ=255) ttCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCaa < 1:1573835/71‑1 (MQ=255) ttCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCaa < 1:421016/71‑1 (MQ=255) ttCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCaa < 1:2285369/71‑1 (MQ=255) ttCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCaa < 1:3480346/71‑1 (MQ=255) gAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGATGGTCAAcc < 1:1244578/52‑1 (MQ=38) gAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGATGGTCAAcc < 1:3365720/52‑1 (MQ=38) gAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGATGGTCAAcc < 1:1335690/52‑1 (MQ=38) aGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGt < 1:1069580/71‑1 (MQ=255) ggATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAAc > 1:1850855/1‑49 (MQ=255) ggATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATa > 1:932827/1‑68 (MQ=255) ggATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATa > 1:1806657/1‑68 (MQ=255) ggATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTg < 1:1532682/71‑1 (MQ=255) aTCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATc < 1:2811979/44‑1 (MQ=255) aTCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATc < 1:3356362/44‑1 (MQ=255) tCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACg < 1:827382/71‑1 (MQ=255) tCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACg > 1:2989142/1‑71 (MQ=255) gcgTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGt < 1:2172123/70‑1 (MQ=255) gcgTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGt < 1:214983/70‑1 (MQ=255) gcgTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGt < 1:384046/70‑1 (MQ=255) gcgTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGt < 1:2684545/70‑1 (MQ=255) gTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTc < 1:1741366/70‑1 (MQ=255) tAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCt > 1:2418888/1‑70 (MQ=255) agagagTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGa > 1:1876400/1‑70 (MQ=255) agagTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATAc < 1:3251063/71‑1 (MQ=255) gTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCt < 1:366619/63‑1 (MQ=255) gTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGc > 1:2830416/1‑71 (MQ=255) tGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCg < 1:32654/71‑1 (MQ=255) gATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGcc < 1:989809/46‑1 (MQ=255) gATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGa > 1:3160162/1‑71 (MQ=255) gATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATAAAGCGGa > 1:764967/1‑71 (MQ=255) gATTCAACGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGt > 1:2382/1‑52 (MQ=255) ttCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGc > 1:491728/1‑66 (MQ=255) ttCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACt < 1:1497602/71‑1 (MQ=255) cACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTc > 1:3248403/1‑71 (MQ=255) ccGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGt < 1:209752/51‑1 (MQ=255) cgcgTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCg < 1:547000/71‑1 (MQ=255) gcgTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCg > 1:2653490/1‑70 (MQ=255) gcgTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCg > 1:1146253/1‑70 (MQ=255) gcgTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCg > 1:1414998/1‑70 (MQ=255) gcgTCCATTGGTTGACCATCAACAAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCg > 1:1200639/1‑70 (MQ=255) tCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAAt < 1:2468886/38‑1 (MQ=255) tCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCa > 1:1021705/1‑69 (MQ=255) cATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAg > 1:2770093/1‑38 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGa > 1:223853/1‑56 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGa > 1:2991157/1‑56 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGa > 1:1202046/1‑56 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCt > 1:3519402/1‑71 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCt > 1:171087/1‑71 (MQ=255) ttGGTTGACCATCAACCAGATTTTGCCCGCAAATAGTGACGTTCTGATACAGCGGa > 1:1617210/1‑56 (MQ=255) tGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATAc > 1:88073/1‑49 (MQ=255) tgTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACtt > 1:3054459/2‑57 (MQ=255) ggTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCtt < 1:1232990/70‑1 (MQ=255) gTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAg < 1:1465563/49‑1 (MQ=255) gTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAg < 1:3112161/49‑1 (MQ=255) gTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAg < 1:1508494/49‑1 (MQ=255) ttGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCagag < 1:695316/64‑1 (MQ=255) ttGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCTTTTg < 1:1887472/71‑1 (MQ=255) ttGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCTTTTg < 1:1931246/71‑1 (MQ=255) tGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCtttt > 1:2863763/1‑69 (MQ=255) gACCATCAACCAGATTTTGCCCGCCAATAGTGACGtt > 1:3312705/1‑37 (MQ=255) gACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGa < 1:2333883/41‑1 (MQ=255) gACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCTTTTGa < 1:610187/70‑1 (MQ=255) gACCATCAACCAGATTTTCCCCGCCAATAGTGACGtt > 1:3737101/1‑37 (MQ=255) gACCATCAACCAGAATTTGCCCGCCAATAGTGACGtt > 1:2971124/1‑37 (MQ=255) | GAACGCAGGCGACCGCAGGATTCGAGGATCGCGTAAGAGAGTGGATTCACCGCGTCCATTGGTTGACCATCAACCAGATTTTGCCCGCCAATAGTGACGTTCTGATACAGCGGACTTCCCGCAGAGGCTTTTGA > Exported/552388‑552521 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |