BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,197	A→T	5.7%	L686Q (CTG→CAG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,197	0	A	T	5.7%	257.7 / 6.0	87	L686Q (CTG→CAG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (31/51); new base T (3/2); total (34/53)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 101 total aligned reads displayed.

AACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACC  >  Exported/608130‑608267

aaCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGt                                                                     >  1:1161895/1‑71 (MQ=255)

 aCGTTGAGGTGTTGACCGCCTTCGACATCCGCTTCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGtt                                                                    <  1:2650862/71‑1 (MQ=255)

 aCGTTCAGGTGCTGACCACCTTCGATGTATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                                    >  1:221652/1‑71 (MQ=255)

 aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                                    >  1:2128310/1‑71 (MQ=255)

 aCGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGtt                                                                    <  1:3235564/71‑1 (MQ=255)

  cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                                                                   <  1:2547285/71‑1 (MQ=255)

  cGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTg                                                                   >  1:3416905/1‑71 (MQ=255)

   gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTgg                                                                  <  1:2992485/71‑1 (MQ=255)

   gTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTgg                                                                  <  1:1385402/71‑1 (MQ=255)

       aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               <  1:1380264/70‑1 (MQ=255)

       aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               <  1:2370936/70‑1 (MQ=255)

       aGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGTCtg                                                              <  1:828901/71‑2 (MQ=39)

          tGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTg                                                                   <  1:1656682/63‑1 (MQ=255)

           gCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCTATCAGACCAGCCAGGTTGGTCTTACg                                                           >  1:3258761/1‑70 (MQ=255)

            cTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGa                                                          <  1:434264/70‑1 (MQ=255)

            cTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGt                                                                 >  1:3086116/1‑63 (MQ=255)

            cTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGt                                                                 >  1:1586726/1‑63 (MQ=255)

            cTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAACCTGGCTGGt                                                                 >  1:895472/1‑63 (MQ=255)

             tGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                                        >  1:1377271/1‑71 (MQ=255)

               accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGt                                                                     <  1:2799403/56‑1 (MQ=255)

               accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACtt                                                      <  1:3205139/71‑1 (MQ=255)

               accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                                       <  1:1969002/70‑1 (MQ=255)

                ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     >  1:831423/1‑71 (MQ=255)

                ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     <  1:599943/71‑1 (MQ=255)

                ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     <  1:1914503/71‑1 (MQ=255)

                ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     <  1:1910382/71‑1 (MQ=255)

                 caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg                                                    <  1:2452448/71‑1 (MQ=255)

                    cTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtcg                                                 >  1:1584842/1‑71 (MQ=255)

                     ttCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGt                                                                     <  1:1420810/50‑1 (MQ=255)

                     ttCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGt                                                                     <  1:2916824/50‑1 (MQ=255)

                     ttCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCtt                                                              <  1:769596/57‑1 (MQ=255)

                       cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                <  1:158074/69‑1 (MQ=255)

                       cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                <  1:3281764/69‑1 (MQ=255)

                       cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                              >  1:1257412/1‑71 (MQ=255)

                        gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                             <  1:2778301/71‑1 (MQ=255)

                         atggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     <  1:547807/62‑1 (MQ=255)

                          tggatgCTTCGTGGTGTAAGTAACCATCCATCAGACCAGCCAGCTTGGTCTTACGa                                                          >  1:1817731/1‑56 (MQ=39)

                           ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                          >  1:978465/1‑71 (MQ=255)

                           ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                          >  1:1056648/1‑71 (MQ=255)

                           ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                          >  1:1084660/1‑71 (MQ=255)

                            gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTAGTCTTTAcc                                         <  1:1333530/71‑1 (MQ=255)

                             atgCTTCGTGGTTGAAGTAACCATCCATCAGACCAGCCAGGTTGGt                                                                 <  1:955561/46‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGt                                                                 <  1:1958557/46‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGt                                                                 <  1:2996878/46‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               <  1:2632856/48‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               <  1:2407937/48‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                        <  1:1049229/71‑1 (MQ=255)

                             atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                        <  1:3641242/71‑1 (MQ=255)

                               gCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                             >  1:151684/1‑48 (MQ=255)

                               gCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                             >  1:621257/1‑48 (MQ=255)

                               gCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                             >  1:1191422/1‑48 (MQ=255)

                               gCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                             >  1:3438648/1‑48 (MQ=255)

                               gCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTa                                                             >  1:1416806/1‑48 (MQ=255)

                                cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                                     <  1:2006578/71‑1 (MQ=255)

                                 ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                    >  1:555346/1‑69 (MQ=255)

                                 ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                    >  1:3317690/1‑69 (MQ=255)

                                     tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                       >  1:1810574/1‑64 (MQ=255)

                                     tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                       >  1:2123364/1‑64 (MQ=255)

                                     ctgtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                 <  1:1079716/68‑1 (MQ=255)

                                        tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               <  1:242005/37‑1 (MQ=255)

                                        tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                 <  1:2592002/67‑1 (MQ=255)

                                        tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                 <  1:1129711/67‑1 (MQ=255)

                                        tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                 <  1:1732766/67‑1 (MQ=255)

                                        tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                             <  1:1470584/71‑1 (MQ=255)

                                         ggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCtt                                                              <  1:651479/37‑1 (MQ=255)

                                          gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               >  1:2901128/1‑35 (MQ=255)

                                          gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCt                                                               >  1:271989/1‑35 (MQ=255)

                                          gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                                        >  1:3705305/1‑42 (MQ=255)

                                          gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                     <  1:2564473/45‑1 (MQ=255)

                                          gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                             <  1:81576/69‑1 (MQ=255)

                                            aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACGCAGTGCGTTCGGAAc                          >  1:3145403/1‑70 (MQ=255)

                                            aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                             <  1:2212706/67‑1 (MQ=255)

                                            aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                             <  1:3243649/67‑1 (MQ=255)

                                                aCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                               <  1:2868811/45‑1 (MQ=255)

                                                  catccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc                          <  1:2684017/64‑1 (MQ=255)

                                                   atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                   >  1:2197010/1‑70 (MQ=255)

                                                   atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                   >  1:2396999/1‑70 (MQ=255)

                                                   atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                  <  1:1198443/71‑1 (MQ=255)

                                                   atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                  <  1:2909350/71‑1 (MQ=255)

                                                     ccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAg                >  1:2451130/1‑71 (MQ=255)

                                                      catcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAgg               >  1:3194138/1‑71 (MQ=255)

                                                         cAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg             >  1:2079794/1‑70 (MQ=255)

                                                          aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt            <  1:1355717/70‑1 (MQ=255)

                                                          aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt            <  1:2219934/70‑1 (MQ=255)

                                                          aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt            <  1:541844/70‑1 (MQ=255)

                                                           gACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg          <  1:189502/71‑1 (MQ=255)

                                                             ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                              >  1:179306/1‑49 (MQ=255)

                                                             ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                              >  1:2560959/1‑49 (MQ=255)

                                                             ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                              >  1:2847790/1‑49 (MQ=255)

                                                             ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg          >  1:470641/1‑69 (MQ=255)

                                                              cagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGGACGATAGAGAAGGTGTAg          >  1:341976/1‑68 (MQ=255)

                                                               agccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg          >  1:3269415/1‑67 (MQ=255)

                                                               agccagGTTGGTCTTACGAACTTCGTCGTCTATACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa           >  1:2142812/1‑66 (MQ=255)

                                                                gccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga      >  1:2956301/1‑70 (MQ=255)

                                                                 ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                   <  1:2029097/56‑1 (MQ=255)

                                                                 ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATa    <  1:3022282/71‑1 (MQ=255)

                                                                  cagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAc   >  1:2464540/1‑71 (MQ=255)

                                                                   agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga      <  1:1493229/67‑1 (MQ=255)

                                                                   agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc  <  1:601451/71‑1 (MQ=255)

                                                                   agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc  <  1:1577235/71‑1 (MQ=255)

AACGTTCAGGTGCTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACC  >  Exported/608130‑608267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`