Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 62,679 A→G 71.4% F260F (TTT→TTC polB ← DNA polymerase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported62,6790AG71.4% 62.3 / 28.4 42F260F (TTT→TTCpolBDNA polymerase II
Reads supporting (aligned to +/- strand):  ref base A (5/7);  new base G (19/11);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT  >  Exported/62613‑62746
                                                                  |                                                                   
tttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGc                                                                 >  1:2690478/1‑71 (MQ=255)
tttCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGc                                                                 >  1:2394792/1‑71 (MQ=255)
       gcCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCg                                                               <  1:2398823/66‑1 (MQ=255)
       gcCTCGATACCGTAGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCt                                                           <  1:2944209/70‑1 (MQ=255)
        ccTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTcg                                                         <  1:23099/71‑1 (MQ=255)
           cGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCgcgc                                                      >  1:1588657/1‑71 (MQ=255)
            gATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCgcgc                                                      >  1:2394126/1‑70 (MQ=255)
               aCCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAg                                                                  >  1:343245/1‑55 (MQ=255)
               aCCGGCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGcc                                                                <  1:1962456/57‑1 (MQ=255)
                   tCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg                                              >  1:1429094/1‑71 (MQ=255)
                    cGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTcc                                                >  1:2585803/1‑68 (MQ=255)
                      aTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCa                                                    >  1:1042706/1‑62 (MQ=255)
                      aTAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCa                                                    >  1:480745/1‑62 (MQ=255)
                       tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTc                                           >  1:946959/1‑70 (MQ=255)
                       tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCg                                          >  1:1610636/1‑71 (MQ=255)
                       tAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCg                                          >  1:1435419/1‑71 (MQ=255)
                         aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg                                              <  1:1749754/65‑1 (MQ=255)
                         aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg                                              <  1:3452304/65‑1 (MQ=255)
                         aTTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAg                                              <  1:2104381/65‑1 (MQ=255)
                           tAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtat                                      >  1:729526/1‑71 (MQ=255)
                             gCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtatt                                     <  1:623827/70‑1 (MQ=255)
                                   cTTTAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTg                                                             <  1:300852/40‑1 (MQ=255)
                                     ttAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc                             >  1:3683902/1‑70 (MQ=255)
                                      tAGCCTGGGCAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGcc                             <  1:2535342/69‑1 (MQ=255)
                                            gggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACg                      >  1:2883401/1‑70 (MQ=255)
                                             ggCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTcc                                                >  1:2814008/1‑43 (MQ=255)
                                               cAAAAAAGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGc                  <  1:3224207/71‑1 (MQ=255)
                                                  aaaaGACGCCGTTTTTGAAGCCGTGCTCGCGCCACt                                                  >  1:117696/1‑36 (MQ=255)
                                                     aGACGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGa            >  1:3140727/1‑71 (MQ=255)
                                                        cGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCgg                <  1:3263583/64‑1 (MQ=255)
                                                        cGCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCgg                <  1:1032486/64‑1 (MQ=255)
                                                         gCCGTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACgg         >  1:360274/1‑70 (MQ=255)
                                                            gTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtattat                                   >  1:3548458/1‑41 (MQ=255)
                                                            gTTTTTGAAGCCGTGCTCGCGCCACTCCAGCTCGCtattat                                   >  1:1436777/1‑41 (MQ=255)
                                                             tttttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACg    >  1:257212/1‑71 (MQ=255)
                                                             tttttGAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACg    >  1:2533294/1‑71 (MQ=255)
                                                               tttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGc   >  1:366476/1‑70 (MQ=255)
                                                                ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg                   <  1:1273132/53‑1 (MQ=255)
                                                                ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg                   <  1:1362190/53‑1 (MQ=255)
                                                                ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg                   <  1:3460936/53‑1 (MQ=255)
                                                                ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAg                   <  1:945836/53‑1 (MQ=255)
                                                                ttAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCt  <  1:1900921/70‑1 (MQ=255)
                                                                  |                                                                   
TTTCAGCGCCTCGATACCGTCGATAATTAGCCGACCTTTAGCCTGGGCAAAAAAGACGCCGTTTTTAAAGCCGTGCTCGCGCCACTCCAGCTCGCTATTATCGCGCCCAAGACGCAGCGGAAGACGGTAACGCT  >  Exported/62613‑62746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: