Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,211	A→T	5.1%	V681V (GTT→GTA)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,211	0	A	T	5.1%	280.7 / 2.1	93	V681V (GTT→GTA)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (36/52);  new base T (2/3);  total (38/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTA  >  Exported/608142‑608279                                                                      |                                                                     cTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGa                                                                      <  1:434264/70‑1 (MQ=255)  tGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                                                    >  1:1377271/1‑71 (MQ=255)    accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACtt                                                                  <  1:3205139/71‑1 (MQ=255)    accaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACt                                                                   <  1:1969002/70‑1 (MQ=255)     ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 >  1:831423/1‑71 (MQ=255)     ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 <  1:599943/71‑1 (MQ=255)     ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 <  1:1914503/71‑1 (MQ=255)     ccaccTTCGATGGATGCTTCGTGGTGGAAGTAACAATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 <  1:1910382/71‑1 (MQ=255)      caccTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcg                                                                <  1:2452448/71‑1 (MQ=255)         cTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtcgtcg                                                             >  1:1584842/1‑71 (MQ=255)            cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                            <  1:3281764/69‑1 (MQ=255)            cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTcgtcgt                                                            <  1:158074/69‑1 (MQ=255)            cGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCt                                                          >  1:1257412/1‑71 (MQ=255)             gatggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCtt                                                         <  1:2778301/71‑1 (MQ=255)              atggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 <  1:547807/62‑1 (MQ=255)               tggatgCTTCGTGGTGTAAGTAACCATCCATCAGACCAGCCAGCTTGGTCTTACGa                                                                      >  1:1817731/1‑56 (MQ=39)                ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                      >  1:1056648/1‑71 (MQ=255)                ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                      >  1:978465/1‑71 (MQ=255)                ggatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAc                                                      >  1:1084660/1‑71 (MQ=255)                 gatgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTAGTCTTTAcc                                                     <  1:1333530/71‑1 (MQ=255)                  atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                                    <  1:1049229/71‑1 (MQ=255)                  atgCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTAccc                                                    <  1:3641242/71‑1 (MQ=255)                     cTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGt                                                 <  1:2006578/71‑1 (MQ=255)                      ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                                >  1:3317690/1‑69 (MQ=255)                      ttCGTGGTGGAAATACCCATCCAGCAGGCCGACAAGGTTGGTTTTACGTACTGGATCTTCTTTGCCCAgcg                                                >  1:555346/1‑69 (MQ=255)                          tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                                   >  1:1810574/1‑64 (MQ=255)                          tggtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCa                                                   >  1:2123364/1‑64 (MQ=255)                          ctgtggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                             <  1:1079716/68‑1 (MQ=255)                             tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                             <  1:2592002/67‑1 (MQ=255)                             tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                             <  1:1732766/67‑1 (MQ=255)                             tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGt                                             <  1:1129711/67‑1 (MQ=255)                             tggAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                         <  1:1470584/71‑1 (MQ=255)                               gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAAc                                                                    >  1:3705305/1‑42 (MQ=255)                               gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTtc                                                                 <  1:2564473/45‑1 (MQ=255)                               gAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                         <  1:81576/69‑1 (MQ=255)                                 aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACGCAGTGCGTTCGGAAc                                      >  1:3145403/1‑70 (MQ=255)                                 aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                         <  1:2212706/67‑1 (MQ=255)                                 aGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCgg                                         <  1:3243649/67‑1 (MQ=255)                                     aCCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCgtcgtc                                                           <  1:2868811/45‑1 (MQ=255)                                       catccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAAc                                      <  1:2684017/64‑1 (MQ=255)                                        atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                               >  1:2396999/1‑70 (MQ=255)                                        atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                               >  1:2197010/1‑70 (MQ=255)                                        atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                              <  1:1198443/71‑1 (MQ=255)                                        atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                              <  1:983901/71‑1 (MQ=255)                                        atccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAgaga                              <  1:2909350/71‑1 (MQ=255)                                          ccatcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAg                            >  1:2451130/1‑71 (MQ=255)                                           catcAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAgg                           >  1:3194138/1‑71 (MQ=255)                                              cAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtg                         >  1:2079794/1‑70 (MQ=255)                                               aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                        <  1:541844/70‑1 (MQ=255)                                               aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                        <  1:1355717/70‑1 (MQ=255)                                               aGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGgtgt                        <  1:2219934/70‑1 (MQ=255)                                                gACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                      <  1:189502/71‑1 (MQ=255)                                                  ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                          >  1:2847790/1‑49 (MQ=255)                                                  ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                          >  1:2560959/1‑49 (MQ=255)                                                  ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCg                                          >  1:179306/1‑49 (MQ=255)                                                  ccagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                      >  1:470641/1‑69 (MQ=255)                                                   cagccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGGACGATAGAGAAGGTGTAg                      >  1:341976/1‑68 (MQ=255)                                                    agccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAg                      >  1:3269415/1‑67 (MQ=255)                                                    agccagGTTGGTCTTACGAACTTCGTCGTCTATACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                       >  1:2142812/1‑66 (MQ=255)                                                     gccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga                  >  1:2956301/1‑70 (MQ=255)                                                      ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATagag                               <  1:2029097/56‑1 (MQ=255)                                                      ccagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATa                <  1:3022282/71‑1 (MQ=255)                                                       cagGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAc               >  1:2464540/1‑71 (MQ=255)                                                        agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGgaga                  <  1:1493229/67‑1 (MQ=255)                                                        agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc              <  1:601451/71‑1 (MQ=255)                                                        agGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATAcc              <  1:1577235/71‑1 (MQ=255)                                                         ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa             <  1:2778570/71‑1 (MQ=255)                                                         ggTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCa             >  1:1274257/1‑71 (MQ=255)                                                          gTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt            <  1:632466/71‑1 (MQ=255)                                                          gTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCAt            <  1:2998289/71‑1 (MQ=255)                                                           ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc           <  1:672893/71‑1 (MQ=255)                                                           ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc           <  1:3163479/71‑1 (MQ=255)                                                           ttGGTTTTACGTACTGGATCTTCTTTGCCCAGCGCCGCAGGAACGATTGAGAAGGTGTACGAGATCCCATc           <  1:2991555/71‑1 (MQ=255)                                                           ttGGTCTTACGAACTTGGTCGTCTTTACCCAGTGCTTTCGGAACGATAGAGAACGTGTAGGAGATACCa             <  1:3379116/69‑1 (MQ=255)                                                           ttGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATc           >  1:3214630/1‑71 (MQ=255)                                                           ttGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATc           >  1:1002129/1‑71 (MQ=255)                                                            tGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt          >  1:3175971/1‑71 (MQ=255)                                                             ggTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt          <  1:3048909/70‑1 (MQ=255)                                                              gTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt         <  1:3229215/70‑1 (MQ=255)                                                               tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATaga                                <  1:225981/46‑1 (MQ=255)                                                               tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCtt         <  1:2306891/69‑1 (MQ=255)                                                               tCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCt          <  1:343722/68‑1 (MQ=255)                                                                cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                 <  1:989949/44‑1 (MQ=255)                                                                cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                 <  1:3274462/44‑1 (MQ=255)                                                                cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                 <  1:2567041/44‑1 (MQ=255)                                                                cTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATag                                 <  1:1673309/44‑1 (MQ=255)                                                                 ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                       >  1:3565558/1‑53 (MQ=255)                                                                 ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTa                       >  1:200017/1‑53 (MQ=255)                                                                 ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGga                    >  1:215671/1‑56 (MQ=255)                                                                 ttACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGc     >  1:2327995/1‑71 (MQ=255)                                                                   aCGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCg    >  1:3613149/1‑70 (MQ=255)                                                                    cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt   <  1:3484541/70‑1 (MQ=255)                                                                    cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt   >  1:2259443/1‑70 (MQ=255)                                                                    cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGt   >  1:1394865/1‑70 (MQ=255)                                                                    cGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTa  <  1:543070/71‑1 (MQ=255)                                                                      |                                                                     CTGACCACCTTCGATGGATGCTTCGTGGTGGAAGTAACCATCCATCAGACCAGCCAGGTTGGTCTTACGAACTTCGTCGTCTTTACCCAGTGCGTTCGGAACGATAGAGAAGGTGTAGGAGATACCATCTTTAGCGTA  >  Exported/608142‑608279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑