Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 723,178 T→C 12.0% T149A (ACC→GCC)  rne ← fused ribonucleaseE endoribonuclease and scaffold for formation of degradosome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported723,1780TC12.0% 131.4 / 9.9 50T149A (ACC→GCC) rnefused ribonucleaseE endoribonuclease and scaffold for formation of degradosome
Reads supporting (aligned to +/- strand):  ref base T (22/22);  new base C (3/3);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

CAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAG  >  Exported/723109‑723248
                                                                     |                                                                      
cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTa                                                                       <  1:2126240/71‑1 (MQ=255)
 aGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTAc                                                                      >  1:3419571/1‑71 (MQ=255)
      tGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcg                                                                 <  1:2024618/71‑1 (MQ=255)
              aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGcc                                                            <  1:1961621/68‑1 (MQ=255)
               tAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCg                                                        <  1:2200781/71‑1 (MQ=255)
                 aGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGAt                                                      <  1:809831/71‑1 (MQ=255)
                  gCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGgtcgtc                                                               <  1:33670/61‑1 (MQ=255)
                        tGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTa                                                                       >  1:1871809/1‑47 (MQ=255)
                         gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGc                                                 >  1:2943488/1‑68 (MQ=255)
                          ccTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga                                             <  1:3365211/71‑1 (MQ=255)
                          ccTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgag                                              >  1:2096029/1‑70 (MQ=255)
                           cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                                            <  1:3097443/71‑1 (MQ=255)
                           cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                                            <  1:2506882/71‑1 (MQ=255)
                           cTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaa                                            <  1:2131506/71‑1 (MQ=255)
                            ttCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTc                                                         <  1:426720/57‑1 (MQ=255)
                             tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa                                                       <  1:2078974/58‑1 (MQ=255)
                              ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGaaa                                           <  1:1655508/69‑1 (MQ=255)
                              ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg                                         >  1:3104243/1‑71 (MQ=255)
                              ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGaaa                                           <  1:1351433/69‑1 (MQ=255)
                               cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATa                                                     >  1:1718521/1‑58 (MQ=255)
                                    gTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCgaga                                             >  1:2672832/1‑61 (MQ=255)
                                     ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCgaga                                             <  1:1710693/60‑1 (MQ=255)
                                     ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg                                  <  1:1487525/71‑1 (MQ=255)
                                     ttCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcg                                  <  1:1502009/71‑1 (MQ=255)
                                       cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAAt                                          >  1:1078752/1‑61 (MQ=255)
                                       cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg                                >  1:506822/1‑71 (MQ=255)
                                       cAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc                                 <  1:2717541/70‑1 (MQ=255)
                                        aaGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa                                                       >  1:640201/1‑47 (MQ=255)
                                        aaGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGa                                                       >  1:390135/1‑47 (MQ=255)
                                        aaGGCTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgc                               >  1:2257428/1‑71 (MQ=255)
                                         aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcg                              <  1:2319902/71‑1 (MQ=255)
                                         aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg                                >  1:3045707/1‑69 (MQ=255)
                                         aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcg                                >  1:261677/1‑69 (MQ=255)
                                          ggCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgcgcgc                             <  1:291688/71‑1 (MQ=255)
                                            cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCgg                           >  1:3273684/1‑71 (MQ=255)
                                            cTTGCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATa                                                     >  1:410491/1‑45 (MQ=255)
                                               gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCcgc                                 >  1:695376/1‑62 (MQ=255)
                                               gCCAGTGCTTCTTTTAATTCGGCACGGTCGTCGCCTTCGATa                                                     <  1:1520116/42‑1 (MQ=255)
                                                 cAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATg                                         >  1:2430196/1‑52 (MQ=255)
                                                      cttcttTTAATTCGGTACGGTCGTCGCCTTCGATACg                                                   >  1:140194/1‑37 (MQ=255)
                                                      cttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCgg                  >  1:3565498/1‑70 (MQ=255)
                                                      cttcttTTAATTCGGCACGGTCGTCGCCTTCGATACGGCGAGaa                                            <  1:3358839/44‑1 (MQ=255)
                                                        tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGgttgtag                    >  1:1408868/1‑64 (MQ=255)
                                                        tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt               >  1:3218098/1‑71 (MQ=255)
                                                         cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt               <  1:1705933/70‑1 (MQ=255)
                                                          ttttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCAt               >  1:3106038/1‑69 (MQ=255)
                                                            ttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAg            >  1:275913/1‑70 (MQ=255)
                                                               aTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAAcc        <  1:401396/71‑1 (MQ=255)
                                                                 tCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGcc                                       >  1:1558079/1‑38 (MQ=255)
                                                                    gTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTAGGCATCAGAACCAGAt    <  1:2664584/70‑1 (MQ=255)
                                                                     tACGGTAGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAg  >  1:1679804/1‑71 (MQ=255)
                                                                     |                                                                      
CAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAG  >  Exported/723109‑723248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: