| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 780,667 | T→C | 58.1% | D125D (GAT→GAC) | ycgM → | predicted isomerase/hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 780,667 | 0 | T | C | 58.1% | 13.4 / 35.1 | 31 | D125D (GAT→GAC) | ycgM | predicted isomerase/hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (5/8); new base C (11/7); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATAC > Exported/780602‑780729 | ttGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAAct < 1:1345569/70‑1 (MQ=255) cGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCtt < 1:545327/70‑1 (MQ=255) cGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCtt < 1:45630/70‑1 (MQ=255) tGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTc < 1:356161/71‑1 (MQ=255) tGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTc > 1:2001011/1‑71 (MQ=255) aTGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTcc < 1:3711554/70‑1 (MQ=255) gTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCt > 1:1120275/1‑71 (MQ=255) aGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTcc < 1:1458129/71‑1 (MQ=255) gAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCggg > 1:3737286/1‑71 (MQ=255) aaaTGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGt > 1:2266411/1‑56 (MQ=255) aTGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTAt < 1:3622052/71‑1 (MQ=255) gaagaaAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTcc < 1:3230391/54‑1 (MQ=255) aagaaAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTcc < 1:1629600/71‑1 (MQ=255) aaaGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCcgc > 1:3428958/1‑71 (MQ=255) aGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCgcgg < 1:2380165/71‑1 (MQ=255) cGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGcggcgg < 1:2335409/71‑1 (MQ=255) gggCAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGcggcgg > 1:2341050/1‑70 (MQ=255) cAGCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAAtt > 1:1543873/1‑71 (MQ=255) gCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTa < 1:732465/71‑1 (MQ=255) gCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTa < 1:371823/71‑1 (MQ=255) gCCGTGGGAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTa > 1:2980566/1‑71 (MQ=255) tGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAAttt > 1:1456720/1‑66 (MQ=255) tGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTa > 1:1718513/1‑67 (MQ=255) tGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTa > 1:672684/1‑67 (MQ=255) ggAAAAGGCTAAAGCGTTTGACAACTCTTGTCCGCtt > 1:356220/1‑37 (MQ=255) aaaGGCTAAAGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGAcc > 1:398448/1‑71 (MQ=255) gtcTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTc > 1:2354668/3‑44 (MQ=255) aaGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCaaaa < 1:1045229/70‑1 (MQ=255) aGCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCcgc > 1:2891699/1‑42 (MQ=255) gCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATa > 1:265807/1‑70 (MQ=255) gCGTTTGACAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATAc < 1:2164621/71‑1 (MQ=255) | TTGCGTGATGTTCAGGGAAAAATGAAGAAAGCCGGGCAGCCGTGGGAAAAGGCTAAAGCGTTTGATAACTCTTGTCCGCTTTCCGGGTTTATTCCCGCGGCGGAATTTACCGGCGACCCGCAAAATAC > Exported/780602‑780729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |