Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 792,959 T→C 6.5% I443V (ATT→GTT)  cvrA ← predicted cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported792,9590TC 6.5% 270.9 / 8.0 92I443V (ATT→GTT) cvrApredicted cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (48/38);  new base C (2/4);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

ACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGA  >  Exported/792889‑793029
                                                                      |                                                                      
acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                                                        <  1:1622244/71‑1 (MQ=255)
acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                                                        <  1:2747909/71‑1 (MQ=255)
acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                                                        <  1:1890368/71‑1 (MQ=255)
acaTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAAt                                                                        <  1:3679739/71‑1 (MQ=255)
 caTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATa                                                                       <  1:3680946/71‑1 (MQ=255)
 caTCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATa                                                                       >  1:155704/1‑71 (MQ=255)
     gCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg                                                                   >  1:3169493/1‑71 (MQ=255)
     gCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATAcgcg                                                                   >  1:2240018/1‑71 (MQ=255)
       cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtc                                                                 >  1:1712626/1‑71 (MQ=255)
       cTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                  >  1:1968074/1‑70 (MQ=255)
         tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc                                                               >  1:1065465/1‑71 (MQ=255)
         tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc                                                               >  1:1079260/1‑71 (MQ=255)
         tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc                                                               >  1:193429/1‑71 (MQ=255)
         tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc                                                               >  1:1336352/1‑71 (MQ=255)
         tCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtctc                                                               >  1:232508/1‑71 (MQ=255)
           gcgcAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtct                                                                >  1:560536/1‑68 (MQ=255)
              cAGTCGGGTGCTGACGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCtttt                                                           >  1:119954/1‑70 (MQ=255)
                    ggTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt                                                             <  1:3642306/62‑1 (MQ=255)
                    ggTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt                                                             <  1:3184558/62‑1 (MQ=255)
                     gTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg                                                   <  1:201795/71‑1 (MQ=255)
                     gTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg                                                   <  1:2632840/71‑1 (MQ=255)
                      tgctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGTCATATGc                                                  <  1:1492866/71‑1 (MQ=255)
                      tgctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATg                                                   >  1:1082112/1‑70 (MQ=255)
                       gctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt                                                             <  1:2035396/59‑1 (MQ=255)
                       gctgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGAGTCTCtt                                                             <  1:3038828/59‑1 (MQ=255)
                         tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtttt                                                           >  1:1602719/1‑59 (MQ=255)
                         tgcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt                                                            >  1:848966/1‑58 (MQ=255)
                          gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt                                              >  1:851288/1‑71 (MQ=255)
                          gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt                                              >  1:2493095/1‑71 (MQ=255)
                          gcCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAAt                                              >  1:1873080/1‑71 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTctct                                                              <  1:3207917/54‑1 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTctct                                                              <  1:2417147/54‑1 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa                                               <  1:2283003/69‑1 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc                                             >  1:3073932/1‑71 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc                                             <  1:3621053/71‑1 (MQ=255)
                           ccGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATc                                             <  1:1938564/71‑1 (MQ=255)
                              gTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                  <  1:2848842/47‑1 (MQ=255)
                              gTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                  <  1:2516428/47‑1 (MQ=255)
                               tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGt                                                                  <  1:2133838/46‑1 (MQ=255)
                               tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg                                          >  1:585649/1‑70 (MQ=255)
                               tAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATcgcg                                          >  1:644120/1‑70 (MQ=255)
                                aGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGtct                                                                >  1:1209474/1‑47 (MQ=255)
                                 ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg                                       <  1:62047/71‑1 (MQ=255)
                                 ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg                                       >  1:220998/1‑71 (MQ=255)
                                 ggATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAg                                       >  1:698983/1‑71 (MQ=255)
                                    tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt                                                            >  1:3496105/1‑47 (MQ=255)
                                    tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCttt                                                            >  1:1444253/1‑47 (MQ=255)
                                    tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCaaa                                               >  1:1928597/1‑60 (MQ=255)
                                    tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGc                                    >  1:2886290/1‑71 (MQ=255)
                                    tGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGc                                    >  1:1008415/1‑71 (MQ=255)
                                      aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGcc                                   <  1:864595/70‑1 (MQ=255)
                                      aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg                                  <  1:2834723/71‑1 (MQ=255)
                                      aaGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcg                                  <  1:1466947/71‑1 (MQ=255)
                                        gCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtt                                                             <  1:1051737/42‑1 (MQ=255)
                                        gCAACTGGTTATCACGAAACAGTGCCGCAATACGCGGCTCTTTTGGCATATGCAAATCGCGCAGTGCcgcg                                <  1:3621203/71‑1 (MQ=255)
                                         cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc                                                  <  1:2567685/52‑1 (MQ=255)
                                         cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc                                                  <  1:2208956/52‑1 (MQ=255)
                                         cAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGc                                                  <  1:1993530/52‑1 (MQ=255)
                                          aaCTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGcc                              >  1:634550/1‑71 (MQ=255)
                                           aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCtttt                                                           <  1:3402942/41‑1 (MQ=255)
                                           aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGt                                      >  1:814650/1‑62 (MQ=255)
                                           aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGt                                      >  1:2113095/1‑62 (MQ=255)
                                           aCTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGccc                             <  1:1673288/71‑1 (MQ=255)
                                            cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa                            <  1:1324449/71‑1 (MQ=255)
                                            cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa                            >  1:1486678/1‑71 (MQ=255)
                                            cTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCa                            <  1:3115980/71‑1 (MQ=255)
                                            cTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGc                                                        <  1:2554920/43‑1 (MQ=255)
                                            cTGGTTATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGc                                                        <  1:1602248/43‑1 (MQ=255)
                                              ggTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg                                     >  1:1950953/1‑60 (MQ=255)
                                              ggTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACg                          >  1:876201/1‑71 (MQ=255)
                                                ttATCACGAAACAGTGCCGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCcgc                                 <  1:3477933/62‑1 (MQ=255)
                                                  aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc                                         <  1:3538933/52‑1 (MQ=255)
                                                  aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc                                         <  1:873221/52‑1 (MQ=255)
                                                  aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCgcgc                                         <  1:2199420/52‑1 (MQ=255)
                                                  aTCACGAAACAGTGCCGCAATACGCGTCTCTTTTGCATATGCAAATCgcgc                                         <  1:3388436/51‑1 (MQ=255)
                                                   tCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCAcc                      <  1:2267553/70‑1 (MQ=255)
                                                   tCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCAcc                      <  1:2119595/70‑1 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:2498561/1‑48 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:1485154/1‑48 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:923866/1‑48 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:3642296/1‑48 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:1897238/1‑48 (MQ=255)
                                                       gAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCa                                        >  1:1575955/1‑48 (MQ=255)
                                                        aaaCAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt                <  1:1877754/71‑1 (MQ=255)
                                                             gTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg                                     <  1:1079223/45‑1 (MQ=255)
                                                             gTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTg                                     <  1:2707275/45‑1 (MQ=255)
                                                              tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt                >  1:3379971/1‑65 (MQ=255)
                                                              tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt                >  1:2397932/1‑65 (MQ=255)
                                                              tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt                >  1:1953057/1‑65 (MQ=255)
                                                              tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTAt                >  1:1297127/1‑65 (MQ=255)
                                                              tGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCAc          >  1:1938431/1‑71 (MQ=255)
                                                                 cGCAACACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCa       <  1:989668/71‑1 (MQ=255)
                                                                    aaTACGCGTCTCTTTTGGCATATGTAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAAtt    >  1:1605743/1‑71 (MQ=255)
                                                                    aaTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAAt     >  1:708624/1‑70 (MQ=255)
                                                                     aTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa                        >  1:3573543/1‑50 (MQ=255)
                                                                     aTACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCa                        >  1:1984093/1‑50 (MQ=255)
                                                                      tACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGa  <  1:2739216/71‑1 (MQ=255)
                                                                      |                                                                      
ACATCGCCTTCGCGCAGTCGGGTGCTGCCGGTAGGATGAAGCAACTGGTTATCACGAAACAGTGCCGCAATACGCGTCTCTTTTGGCATATGCAAATCGCGCAGTGCCGCGCCCACGCACCATTTATCGGCACTCAATTGA  >  Exported/792889‑793029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: