Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 4,371,271 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0090484,371,2710A.100.0% 289.9 / NA 70intergenic (‑6/+300)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (35/35);  total (35/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*AP0090484,371,2720A.100.0% 289.9 / NA 70intergenic (‑7/+299)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (35/35);  total (35/35)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAA  >  AP009048/4371202‑4371335
                                                                     ||                                                               
ggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa                                                                 <  1:991149/71‑1 (MQ=255)
ggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCAAGCAACCTGCATGGCAGaa                                                                 <  1:2455368/71‑1 (MQ=255)
ggTTACGGCGCAGCAGCTTTTCTGCCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa                                                                 <  1:4510073/71‑1 (MQ=255)
 gTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGa                                                                  <  1:1284239/69‑1 (MQ=255)
  ttACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa                                                                <  1:3543523/70‑1 (MQ=255)
   tACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa                                                                >  1:176418/1‑69 (MQ=255)
    aCGGCGCAGCAGCTTTTCTGTCTGATGAACCATATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaata                                                              <  1:4032316/70‑3 (MQ=255)
          cagcagCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGa                                                     >  1:289450/1‑71 (MQ=255)
          cagcagCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGa                                                     <  1:1433622/71‑1 (MQ=255)
           agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                                    <  1:4209265/71‑1 (MQ=255)
           agcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                                    <  1:3167387/71‑1 (MQ=255)
            gcagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                   <  1:3608743/71‑1 (MQ=255)
            gcagcTTTTCTGTCTGATGAACC‑‑ATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAc                                                 <  1:2088806/71‑1 (MQ=255)
             cagcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaa                                                                >  1:931361/1‑59 (MQ=255)
              agcTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAAc                                                 <  1:1826421/71‑1 (MQ=255)
                cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                               <  1:3324362/71‑1 (MQ=255)
                cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                               >  1:856035/1‑71 (MQ=255)
                   ttCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc                                              <  1:1074361/69‑1 (MQ=255)
                    tctttctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCAt                                           >  1:4282408/1‑71 (MQ=255)
                    tctgtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCAt                                           >  1:483661/1‑71 (MQ=255)
                     ctgtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCAt                                           <  1:1610390/70‑1 (MQ=255)
                        tctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGc                                              <  1:3206788/64‑1 (MQ=255)
                        tctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGAt                                        <  1:4466293/70‑1 (MQ=255)
                          tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCAt                                           <  1:1901033/65‑1 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                      <  1:672501/68‑1 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    <  1:4686240/70‑1 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    >  1:3178824/1‑70 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    >  1:3408765/1‑70 (MQ=255)
                             tgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    >  1:610281/1‑69 (MQ=255)
                              gaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAgcggcg                                                      >  1:1112359/1‑50 (MQ=255)
                              gaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAgcggcg                                                      >  1:3745995/1‑50 (MQ=255)
                              gaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    >  1:4974159/1‑68 (MQ=255)
                              gaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                    >  1:3771260/1‑68 (MQ=255)
                               aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                                    <  1:945504/51‑1 (MQ=255)
                               aaCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGgag                                 <  1:733123/70‑1 (MQ=255)
                                aCCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaaat                                                               <  1:3624064/41‑2 (MQ=255)
                                   aGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa                             >  1:1462641/1‑70 (MQ=255)
                                    gATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACa                           >  1:415453/1‑71 (MQ=255)
                                    gATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACa                           >  1:4235751/1‑71 (MQ=255)
                                     aTAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACa                           >  1:1050735/1‑70 (MQ=255)
                                        gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                        >  1:202538/1‑70 (MQ=255)
                                        gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGa                       <  1:2533536/71‑1 (MQ=255)
                                         tCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGAc                            <  1:2689354/65‑1 (MQ=255)
                                           cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                      >  1:647364/1‑53 (MQ=255)
                                           cAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAAc                     <  1:1669040/70‑1 (MQ=255)
                                            aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa                      >  1:3419553/1‑68 (MQ=255)
                                            aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                   <  1:357178/71‑1 (MQ=255)
                                            aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                   <  1:1910174/71‑1 (MQ=255)
                                            aGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                   <  1:2972515/71‑1 (MQ=255)
                                             gACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                        <  1:254635/65‑1 (MQ=255)
                                             gACCGCAAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg                  <  1:2148250/71‑1 (MQ=255)
                                               ccGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGAt                <  1:1830814/71‑1 (MQ=255)
                                                cGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGAt                <  1:5136855/70‑1 (MQ=255)
                                                cGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATg               >  1:1899415/1‑71 (MQ=255)
                                                  ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                   >  1:2318371/1‑65 (MQ=255)
                                                  ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGtt             <  1:4166957/71‑1 (MQ=255)
                                                  ccAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGt              >  1:2726710/1‑70 (MQ=255)
                                                   cAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTa            >  1:1919204/1‑71 (MQ=255)
                                                    aGCAACCTGCATGGCAG‑‑AAATAGCGGCGCTAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAc           >  1:2868941/1‑71 (MQ=255)
                                                     gCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGa                 >  1:1278580/1‑64 (MQ=255)
                                                     gCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTAcc          >  1:2426777/1‑71 (MQ=255)
                                                      cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa                             >  1:1523234/1‑51 (MQ=38)
                                                      cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa                             >  1:3096419/1‑51 (MQ=38)
                                                      cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa                             >  1:5159816/1‑51 (MQ=38)
                                                      cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACgg                   <  1:2679550/61‑1 (MQ=255)
                                                      cAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCg         >  1:4903134/1‑71 (MQ=255)
                                                       aaCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                      >  1:3449069/1‑41 (MQ=37)
                                                        aCCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCggg       >  1:1505306/1‑71 (MQ=255)
                                                         ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                        <  1:3956158/53‑1 (MQ=255)
                                                         ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                        <  1:588664/53‑1 (MQ=255)
                                                         ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGt      <  1:4083375/71‑1 (MQ=255)
                                                          cTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                      >  1:4052936/1‑38 (MQ=25)
                                                           tGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCggg       <  1:5129856/68‑1 (MQ=255)
                                                            gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGaa                      >  1:4330828/1‑52 (MQ=38)
                                                            gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACggg                  <  1:1279655/56‑1 (MQ=255)
                                                            gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGttt    >  1:5102204/1‑70 (MQ=255)
                                                             cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGttt    <  1:3131905/69‑1 (MQ=255)
                                                             cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTaa  >  1:2875945/1‑71 (MQ=255)
                                                             cATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTa   <  1:2737630/70‑1 (MQ=255)
                                                                     ||                                                               
GGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTTTAA  >  AP009048/4371202‑4371335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: