| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 70,740 | T→G | 20.4% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 70,740 | 0 | T | G | 20.4% | 80.9 / 35.1 | 59 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (22/25); new base G (6/6); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.20e-01 | |||||||||||
TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > AP009048/70673‑70806 | tttACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttc < 1:1094871/71‑1 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt > 1:943840/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttctt > 1:2666879/1‑71 (MQ=255) tACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:2236746/70‑1 (MQ=255) aCTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttcttt > 1:4295748/1‑71 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt < 1:1830874/69‑1 (MQ=255) ttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:4484649/62‑1 (MQ=255) ttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:1692918/62‑1 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCg > 1:1358566/1‑69 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:4320031/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:1793602/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:4757972/1‑71 (MQ=255) tCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGcc > 1:3083280/1‑71 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGccc > 1:348627/1‑71 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:2107922/60‑1 (MQ=255) cgcgcgCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa < 1:1388628/70‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:3845053/71‑1 (MQ=255) gcgcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg < 1:4106264/71‑1 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATg > 1:4875297/1‑69 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGa < 1:2882129/70‑1 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:4545187/55‑1 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgc > 1:405441/1‑71 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgc < 1:4187779/71‑1 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAg < 1:4733366/70‑1 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:4351299/71‑1 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt > 1:2003815/1‑46 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt > 1:4461804/1‑46 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcgc < 1:779543/68‑1 (MQ=255) tGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:4419985/49‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:9109/1‑47 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGaa < 1:3879029/61‑1 (MQ=255) gCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCa < 1:3665051/69‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAg > 1:3137125/1‑69 (MQ=255) aTGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct < 1:3848456/45‑1 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcg < 1:2915913/58‑1 (MQ=255) aTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAtt > 1:1336791/1‑71 (MQ=255) tGGCTTATCTGGCCGTCAATATTTGCGAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:3677752/71‑1 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttctttct > 1:190117/1‑43 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:4101649/71‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:5101282/1‑58 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa > 1:4340819/1‑48 (MQ=255) tAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa > 1:1005434/1‑68 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg > 1:3677726/1‑70 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc < 1:1546665/71‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAc > 1:1629556/1‑71 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGc < 1:3557586/67‑1 (MQ=255) cTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAcc > 1:3204386/1‑71 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt > 1:459224/1‑71 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:2475554/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt > 1:4583343/1‑71 (MQ=255) ccGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:2087507/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:231834/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:3041848/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:4178955/71‑1 (MQ=255) cGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGttt < 1:4462148/71‑1 (MQ=255) gTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGAt < 1:4771188/37‑1 (MQ=255) gTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTg > 1:13040/1‑71 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg < 1:5147075/71‑1 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:3210472/1‑70 (MQ=255) aaTATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:3772194/1‑69 (MQ=255) atatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc < 1:3990562/42‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:372991/64‑1 (MQ=255) tatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa > 1:2214464/1‑70 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATc < 1:3772208/70‑1 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa > 1:5005015/1‑71 (MQ=255) tGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCa > 1:1063051/1‑71 (MQ=255) cAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCAtt < 1:1042933/70‑1 (MQ=255) cAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTa < 1:865112/71‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:242873/43‑1 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt > 1:4664303/1‑55 (MQ=255) aTACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc > 1:2798433/1‑71 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:414184/55‑1 (MQ=255) aCGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc > 1:2520072/1‑35 (MQ=255) aCGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc > 1:1206057/1‑35 (MQ=255) cGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGcc < 1:33778/71‑1 (MQ=255) | TTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC > AP009048/70673‑70806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |