| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,312 | G→A | 100% | pseudogene (810/1645 nt) pseudogene (810/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,312 | 0 | G | A | 100.0% | 215.4 / NA | 75 | pseudogene (810/1645 nt) pseudogene (810/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (36/39); total (36/39) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCC > AP009048/3685245‑3685377 | tatGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCAcc < 1:3213817/70‑1 (MQ=255) tGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCAc < 1:3936508/71‑1 (MQ=255) gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg < 1:758020/71‑1 (MQ=255) gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACgg > 1:1342044/1‑71 (MQ=255) tAACACTGGCGTGCGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGc > 1:1260536/1‑70 (MQ=38) acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt > 1:4560832/1‑71 (MQ=255) acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt < 1:2740506/71‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa < 1:5163421/71‑2 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa < 1:3374351/71‑2 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc > 1:4262953/1‑69 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATca < 1:4016201/67‑1 (MQ=255) gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:338256/71‑1 (MQ=39) gtgtGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt > 1:1827315/1‑71 (MQ=39) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaag < 1:2695586/67‑3 (MQ=38) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg < 1:4942132/70‑2 (MQ=38) gtgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:1200475/69‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt < 1:4747600/70‑1 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:3990593/2‑71 (MQ=38) tgcgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACt > 1:1801817/2‑71 (MQ=38) tgcgAAGGGGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagca < 1:5114602/61‑1 (MQ=38) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc > 1:4867980/1‑71 (MQ=39) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc > 1:3247028/1‑71 (MQ=39) gAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCgcac < 1:1544752/67‑1 (MQ=38) gAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCt < 1:247211/71‑1 (MQ=39) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt < 1:448059/53‑1 (MQ=255) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcag < 1:2363627/56‑1 (MQ=255) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcag < 1:486723/56‑1 (MQ=255) aGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc < 1:4383247/71‑1 (MQ=39) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAc < 1:4641247/71‑1 (MQ=39) tgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagca < 1:2587252/54‑1 (MQ=255) tCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa < 1:2310606/39‑1 (MQ=255) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa > 1:4877998/1‑50 (MQ=255) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaa > 1:4903781/1‑50 (MQ=255) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:1974466/70‑1 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:1220646/70‑1 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:791781/1‑70 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg > 1:1756024/1‑69 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGa < 1:4689141/70‑1 (MQ=38) tttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:3182603/71‑1 (MQ=39) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttc > 1:3886749/1‑70 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:4638580/70‑3 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:2791040/70‑3 (MQ=255) ggTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa > 1:4101647/1‑63 (MQ=38) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:876138/1‑71 (MQ=255) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:1369833/1‑71 (MQ=255) tCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:4487747/71‑1 (MQ=255) tCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt > 1:2917296/1‑71 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAGGTTCGCCGCtt > 1:4715110/1‑71 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt > 1:4754213/1‑71 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg < 1:2238785/42‑2 (MQ=25) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:1541728/71‑1 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt > 1:4617108/1‑71 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCttt < 1:1056266/70‑1 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:5080745/1‑71 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:2770940/1‑71 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:4355081/71‑1 (MQ=255) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTgg > 1:3243188/1‑71 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc > 1:501349/1‑70 (MQ=255) atcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGccat < 1:1502340/71‑3 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:3298459/1‑52 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAc < 1:5007782/42‑1 (MQ=37) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg > 1:2787486/1‑61 (MQ=255) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAt > 1:1705933/1‑71 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGa > 1:4284679/1‑45 (MQ=38) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:1081978/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:698636/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1055331/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:1968242/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt < 1:2551105/71‑2 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAtt > 1:4765006/1‑70 (MQ=255) aaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTCGGTGCCATGCAtt > 1:710355/1‑70 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4683121/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4676943/1‑69 (MQ=255) cGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCCCTTTGGTGCCATGCATtcc < 1:4503563/71‑1 (MQ=255) gTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc > 1:505713/1‑70 (MQ=255) | TATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCC > AP009048/3685245‑3685377 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |