| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,072 | C→T | 100% | A142A (GCC→GCT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,072 | 0 | C | T | 100.0% | 231.0 / NA | 78 | A142A (GCC→GCT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (31/47); total (31/47) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825006‑3825139 | aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGTTTCGCGAATCGATGGAGATTATTCAGGCTAATg > 1:2773843/1‑71 (MQ=255)aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:2935818/70‑1 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:975689/1‑71 (MQ=255) aTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg < 1:1547425/71‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcg > 1:2789711/1‑70 (MQ=255) gTAGATGATGTGATCAGCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:2111416/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:5110054/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGcgcg < 1:3002781/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGAc > 1:4288239/1‑71 (MQ=255) tAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACg < 1:4907272/71‑1 (MQ=255) gatgatGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGc < 1:3714681/70‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:409352/60‑1 (MQ=255) atgatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCtt < 1:1294167/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg > 1:4554703/1‑71 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg < 1:3471435/71‑1 (MQ=255) tgtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTg > 1:4751754/1‑71 (MQ=255) gtgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTgg > 1:3186607/1‑71 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGc < 1:1002157/67‑1 (MQ=255) tgATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTACTCAGGCTAATgg > 1:3625188/1‑55 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:1666408/71‑2 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5083493/71‑2 (MQ=255) tCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgta < 1:5143105/71‑2 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:990384/67‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:598691/71‑1 (MQ=255) ccgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTAtt < 1:4398871/70‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGGTGGCGTATTGa > 1:890219/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt > 1:5067526/1‑66 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:1420943/1‑71 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa > 1:3307288/1‑71 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt < 1:567019/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt > 1:3659965/1‑71 (MQ=255) gcgGTACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATg < 1:2706552/44‑1 (MQ=37) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt < 1:1176174/64‑1 (MQ=255) ccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCgt < 1:1234933/64‑1 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATgg > 1:3320328/1‑45 (MQ=38) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg > 1:4421202/1‑71 (MQ=255) aaCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:2876051/71‑1 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg < 1:454432/69‑1 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:4540550/71‑2 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCttg < 1:4885902/71‑3 (MQ=255) ggCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1591931/1‑69 (MQ=255) cGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:4939862/1‑71 (MQ=255) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg < 1:900332/53‑1 (MQ=39) gATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:4188034/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGc < 1:4451041/50‑1 (MQ=39) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1307027/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:4841797/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:2974398/70‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:3794901/70‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:557728/70‑1 (MQ=255) gcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:2408887/1‑58 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:2111461/1‑69 (MQ=255) cgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:4351201/71‑1 (MQ=255) gaatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGa < 1:2676720/68‑1 (MQ=255) aatCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGaa > 1:486598/3‑71 (MQ=255) atCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:3211332/70‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:1901699/62‑1 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:4035541/1‑65 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:49131/1‑65 (MQ=255) tCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcg < 1:4945953/71‑1 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGGGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg < 1:4502927/71‑3 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcggg > 1:1258178/1‑69 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:2912654/70‑2 (MQ=255) gATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAcgg < 1:4375421/70‑2 (MQ=255) aTGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAgg < 1:2598413/64‑1 (MQ=255) tGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGgggg < 1:4910219/71‑1 (MQ=255) ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc > 1:2954676/1‑71 (MQ=255) ggAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgc > 1:1833793/1‑71 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc > 1:2046396/1‑55 (MQ=255) agaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcgc > 1:5158911/1‑71 (MQ=255) ttattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:4599990/1‑71 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAAc < 1:1408462/59‑1 (MQ=255) attCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc > 1:2909494/1‑52 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCa > 1:1824494/1‑52 (MQ=255) cAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAt > 1:4052875/1‑71 (MQ=255) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:3474160/39‑1 (MQ=37) aGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAtt < 1:592841/71‑1 (MQ=255) ggCTAATGGCCCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:2476450/71‑1 (MQ=255) | AATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAGTCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGCTCGATCGTCAGGAACGCGGGCGCGGCGAGATTT > AP009048/3825006‑3825139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |