| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,825,216 | C→T | 100% | Y190Y (TAC→TAT) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,825,216 | 0 | C | T | 100.0% | 194.0 / NA | 65 | Y190Y (TAC→TAT) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/37); total (28/37) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGT > AP009048/3825150‑3825277 | ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:1694183/71‑1 (MQ=255)ggAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTg > 1:4322960/1‑70 (MQ=255) aGTTGAGCGTGTTTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAag < 1:1135267/71‑1 (MQ=255) ttGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag < 1:2282409/71‑1 (MQ=255) tGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:2661754/1‑70 (MQ=255) tGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAag > 1:2354713/1‑68 (MQ=255) gAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:4377842/1‑69 (MQ=255) gAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAagag > 1:2241810/1‑69 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:67620/71‑1 (MQ=255) cGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGcc < 1:1646192/71‑1 (MQ=255) gTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAgaga > 1:4258595/1‑66 (MQ=255) ttACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaaa < 1:2615440/71‑1 (MQ=255) ttACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaa < 1:3539447/70‑1 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAgaga > 1:2594038/1‑61 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:4200201/1‑71 (MQ=255) tACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt > 1:3902058/1‑71 (MQ=255) aCAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa > 1:2874556/1‑67 (MQ=255) aCAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAAt < 1:1318402/70‑1 (MQ=255) cAACTGCTAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:3585312/70‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:1022560/71‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:1742235/71‑1 (MQ=255) cAACTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATgg < 1:4744873/71‑1 (MQ=255) aaCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCgg < 1:1021976/64‑1 (MQ=255) aCTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg < 1:1868467/71‑1 (MQ=255) cTGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:4104293/1‑70 (MQ=255) tGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:44249/49‑1 (MQ=38) tGCAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTgg < 1:4575471/49‑1 (MQ=38) cataGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGa < 1:756402/66‑1 (MQ=255) cAAAGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGa < 1:1369398/69‑1 (MQ=255) aaaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGa < 1:3925388/59‑1 (MQ=38) aaaGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAAc < 1:4320331/70‑1 (MQ=255) aGTGATCTCTATCATCACCCTGAATGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATc > 1:5670/1‑71 (MQ=255) aGTGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCTGGAAATGGCGGAACAt < 1:2347012/70‑1 (MQ=255) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt > 1:1097074/1‑68 (MQ=255) tGATCTCTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTg > 1:1179415/1‑71 (MQ=255) tctcTATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCCGAACAt < 1:12102/65‑1 (MQ=255) tctATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcgg > 1:3988191/1‑70 (MQ=255) ctATCATCACCATGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACAt > 1:1806508/1‑62 (MQ=255) tATCATCACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCt < 1:1672203/63‑1 (MQ=255) atcatcACCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCTGt > 1:3085364/1‑71 (MQ=255) tcatcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGtt < 1:2607877/71‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTa < 1:5146238/70‑1 (MQ=255) atcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTa < 1:4129509/70‑1 (MQ=255) tcaCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTggcggc > 1:3511601/1‑64 (MQ=38) aCCCTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGaa > 1:2558676/1‑42 (MQ=25) ccTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATg < 1:2986861/43‑1 (MQ=25) cTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCg > 1:4448692/1‑45 (MQ=37) cTGAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTa < 1:3513343/71‑1 (MQ=255) gAAAGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATc > 1:3041606/1‑71 (MQ=255) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGTAAATGGCGGAAc > 1:448469/1‑46 (MQ=25) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTaa > 1:96517/1‑62 (MQ=38) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcg > 1:1456536/1‑71 (MQ=255) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcg > 1:1414763/1‑71 (MQ=255) aaaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATc < 1:4844396/70‑1 (MQ=255) aaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAg > 1:3734219/1‑62 (MQ=38) aaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgc < 1:2182828/71‑1 (MQ=255) aaGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgc < 1:62046/71‑1 (MQ=255) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCt > 1:4268967/1‑65 (MQ=39) aGATCTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg < 1:3339955/71‑1 (MQ=255) atcTGATTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATcgcg > 1:3445111/3‑69 (MQ=39) gATTGCCTATCTGGAAGAGAAGCCGGAAATGGCgg < 1:3291003/35‑1 (MQ=25) aTTGCCTATCTGGAAGAGAAGCCGGGAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGt < 1:3955526/70‑1 (MQ=255) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGaa < 1:5081628/66‑1 (MQ=255) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAagag < 1:3410005/69‑1 (MQ=255) aTTGCCTATCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAag < 1:1421428/67‑1 (MQ=255) | GGAAGTTGAGCGTGATTACAACTGCAAAGTGATCTCTATCATCACCCTGAAAGACCTGATTGCTTACCTGGAAGAGAAGCCGGAAATGGCGGAACATCTGGCGGCGGTTAAGGCCTATCGCGAAGAGT > AP009048/3825150‑3825277 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |