| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,864,289 | G→C | 7.0% | S185S (TCC→TCG) | mtlR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,864,289 | 0 | G | C | 7.0% | 210.7 / 5.1 | 71 | S185S (TCC→TCG) | mtlR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base G (38/28); new base C (2/3); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAATTTG > AP009048/3864219‑3864354 | ggCCGAAGCAGGTAGAGGGGCAGTAAGCAGACTTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTg < 1:1248760/71‑1 (MQ=255) gCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTg > 1:619431/1‑70 (MQ=255) cGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGaa < 1:3871603/71‑1 (MQ=255) gAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGaaa < 1:1685423/71‑1 (MQ=255) aaGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAAt > 1:1057622/1‑71 (MQ=255) aaGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAAt > 1:339987/1‑71 (MQ=255) aGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAAt > 1:932681/1‑70 (MQ=255) cAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAg < 1:798164/71‑1 (MQ=255) cAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAg < 1:4981206/71‑1 (MQ=255) agagGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCtcag > 1:805301/1‑71 (MQ=255) agagGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCtcag > 1:4015245/1‑71 (MQ=255) agGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCtcagt > 1:3318741/1‑70 (MQ=255) gCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGa < 1:129368/54‑1 (MQ=255) cAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa < 1:2755227/71‑1 (MQ=255) cAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa < 1:4453672/71‑1 (MQ=255) cAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa < 1:1957853/71‑1 (MQ=255) cAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa < 1:4060674/71‑1 (MQ=255) gTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa > 1:4373337/1‑69 (MQ=255) tAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAg < 1:786948/71‑1 (MQ=255) aaGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGc > 1:3730013/1‑56 (MQ=255) aGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa > 1:1766024/1‑66 (MQ=255) aGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGa > 1:3940401/1‑71 (MQ=255) aGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGa > 1:4736572/1‑71 (MQ=255) aGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTCCAAAATCAGc > 1:3560880/1‑55 (MQ=37) aGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTCCAAAATCAGc > 1:1024181/1‑55 (MQ=37) gCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTCCAAAATCa < 1:1574789/52‑1 (MQ=37) gACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCtcagtc < 1:1089161/57‑1 (MQ=255) gACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAt > 1:993254/1‑71 (MQ=255) gACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAt > 1:2438483/1‑71 (MQ=255) gACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAt > 1:1314826/1‑71 (MQ=255) tGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAttgtt > 1:1060230/1‑70 (MQ=255) tGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAttgtt > 1:84736/1‑70 (MQ=255) tACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGa < 1:425457/68‑1 (MQ=255) tACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCg > 1:2313886/1‑71 (MQ=255) aCTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGaa > 1:2173953/1‑53 (MQ=255) aCTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa > 1:3410272/1‑52 (MQ=255) aCTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCAttgttg > 1:4066434/1‑66 (MQ=255) aCTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCg > 1:2192971/1‑70 (MQ=255) aCTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCg > 1:4109333/1‑70 (MQ=255) tttGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGa < 1:930048/49‑1 (MQ=255) tttGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGca > 1:1326581/1‑69 (MQ=255) ttGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGcac < 1:2012717/69‑1 (MQ=255) ttGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAg > 1:1376303/1‑71 (MQ=255) ttGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAg > 1:1452843/1‑71 (MQ=255) aaaGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGAc > 1:2088397/1‑51 (MQ=255) aaGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTg > 1:3801019/1‑71 (MQ=255) gCTTTTTTTAAGCTGATTTTGGAAATCAGCtcagtcag < 1:1171897/38‑1 (MQ=255) gCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTg < 1:4083739/71‑1 (MQ=255) ttttttAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGcaca > 1:2195712/1‑60 (MQ=255) ttttttAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGa > 1:2401539/1‑69 (MQ=255) ttttttAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGa > 1:2988837/1‑69 (MQ=255) ttttttAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGa > 1:2553281/1‑69 (MQ=255) tttttAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCg < 1:168219/55‑1 (MQ=255) tAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGAt > 1:3673666/1‑49 (MQ=255) tAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGAt > 1:1378079/1‑49 (MQ=255) aaGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGa > 1:83550/1‑47 (MQ=255) cTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCa < 1:2470422/37‑1 (MQ=255) cTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAgcg < 1:1368498/65‑1 (MQ=255) cTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAt < 1:4260996/71‑1 (MQ=255) tGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAg < 1:232379/51‑1 (MQ=255) gATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCGCAGCCTGTTGATAGCGCTGAAttt > 1:4324860/1‑71 (MQ=255) gATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTg > 1:4505317/1‑57 (MQ=255) gATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAt < 1:1524511/69‑1 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAg > 1:1630105/1‑49 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGAt < 1:1279280/58‑1 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAttt < 1:2542662/70‑1 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAttt < 1:3779454/70‑1 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAtt < 1:2787139/69‑1 (MQ=255) aTTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAt < 1:4701073/68‑1 (MQ=255) aTTTTCGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAttt < 1:2341083/70‑1 (MQ=255) aTTTTCGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAAttt < 1:2341081/70‑1 (MQ=255) ttttGGAAATCAGCTCAGTCAGTGAAAGGACCAttgt < 1:624689/37‑1 (MQ=255) ttttGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAATttg > 1:4460851/1‑70 (MQ=255) | GGCCGAAGCAGGTAGAGGGGCAGTAAGCAGACGTTGCTTACTTTTGAAAGGCTTTTTTTAAGCTGATTTTGGAAATCAGCTCAGTCAGTGAAAGGACCATTGTTGATCGCACAGCCTGTTGATAGCGCTGAATTTG > AP009048/3864219‑3864354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |