| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,452,530 | G→C | 6.2% | V1247L (GTA→CTA) | ytfN → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,452,530 | 0 | G | C | 6.2% | 197.1 / 2.6 | 64 | V1247L (GTA→CTA) | ytfN | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (34/26); new base C (2/2); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
TATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTT > AP009048/4452466‑4452598 | tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGa < 1:228375/69‑1 (MQ=255)tataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGAc > 1:381775/1‑70 (MQ=255) ataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTa > 1:2900083/1‑66 (MQ=255) ataGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGa > 1:364462/1‑68 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGAcc < 1:3073247/69‑1 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGAc < 1:3288614/68‑1 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCa > 1:2787027/1‑70 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAg > 1:2823125/1‑71 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAg > 1:4225690/1‑71 (MQ=255) taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAg > 1:4257586/1‑71 (MQ=255) aGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAg < 1:1962120/65‑1 (MQ=255) cAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGCCTGGTCTACACCAGACa < 1:4270169/71‑1 (MQ=38) aaCACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAg < 1:3872444/62‑1 (MQ=255) acacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACt < 1:505772/71‑1 (MQ=255) cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTg > 1:3993306/1‑71 (MQ=255) cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAtt > 1:1263025/1‑71 (MQ=255) cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAtt > 1:3574659/1‑71 (MQ=255) acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttt > 1:3944379/1‑71 (MQ=255) acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAtt < 1:1888161/70‑1 (MQ=255) acgttacgttATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAt < 1:4616915/69‑1 (MQ=255) cgttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTa > 1:7862/1‑53 (MQ=255) gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGc > 1:3166020/1‑71 (MQ=255) gttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGc < 1:4764269/71‑1 (MQ=255) ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGct > 1:3028840/1‑71 (MQ=255) tacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGct > 1:831210/1‑70 (MQ=255) gttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTAt > 1:2184392/1‑71 (MQ=255) ttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATc < 1:1359347/71‑1 (MQ=255) aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttt < 1:719870/61‑1 (MQ=255) aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAg > 1:3889355/1‑71 (MQ=255) tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTa < 1:4493873/66‑1 (MQ=255) tCGCCTGATGCCTAAGCTATATCTGGAAGACGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGt > 1:2799527/1‑71 (MQ=255) cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTa < 1:2339994/65‑1 (MQ=255) gCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGtt < 1:428932/70‑1 (MQ=255) ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTc > 1:3971944/1‑70 (MQ=255) cTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttt > 1:67652/1‑56 (MQ=255) tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAg > 1:5013915/1‑71 (MQ=255) tGATGCCTAAGCTATATCTGGAAGCCGTGCCTGGTCTAGACCAg < 1:2111638/44‑1 (MQ=38) gCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACt < 1:2016711/45‑1 (MQ=255) cTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTa > 1:1774430/1‑70 (MQ=255) aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGtt > 1:3343744/1‑58 (MQ=255) aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCa < 1:4991903/70‑1 (MQ=255) aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCa < 1:1860162/70‑1 (MQ=255) gCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCaa > 1:3087268/1‑70 (MQ=255) gCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAAt < 1:2825162/71‑1 (MQ=255) cTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATg < 1:3102649/71‑1 (MQ=255) cTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATg < 1:1739029/71‑1 (MQ=255) tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAg > 1:2793517/1‑61 (MQ=255) ggAAGCCGTGTCTGGTGTAGACCAGGCACTGGAttt > 1:2466951/1‑36 (MQ=255) gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAata < 1:3083517/68‑1 (MQ=255) gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAata < 1:321085/68‑1 (MQ=255) gAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATAtt < 1:2310630/70‑1 (MQ=255) gCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGa < 1:212205/47‑1 (MQ=255) gCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAg < 1:4549517/48‑1 (MQ=255) gCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGc < 1:2527121/55‑1 (MQ=255) cGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTa > 1:1155360/1‑71 (MQ=255) gtCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATAtt > 1:2827487/1‑62 (MQ=255) gtCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg > 1:4185317/1‑71 (MQ=255) gtCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg > 1:1422961/1‑71 (MQ=255) tCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg > 1:811605/1‑70 (MQ=255) tCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg > 1:4117328/1‑70 (MQ=255) tCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg > 1:2954071/1‑70 (MQ=255) tGGTCTACACCAGACACTGGATTTGCTCTATCAGTTCGAGTTTTAGCaa > 1:104698/1‑49 (MQ=37) ggTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGt > 1:4310403/1‑71 (MQ=255) ggTCTACACCAGACACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACg > 1:1200107/1‑66 (MQ=255) gtgtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCa < 1:1779184/68‑1 (MQ=255) gtgtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGtt < 1:5164024/71‑1 (MQ=255) gtgtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGtt > 1:3893399/1‑71 (MQ=255) gtgtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGtt > 1:4263916/1‑71 (MQ=255) tgtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCaa < 1:2174361/46‑1 (MQ=255) gtAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGtt < 1:5179845/69‑1 (MQ=255) | TATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGGATTTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTT > AP009048/4452466‑4452598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |