Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,892,019:1 +GC 100% intergenic (‑437/+54) gudP ← / ← ygdL predicted D‑glucarate transporter/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,892,0191.G100.0% 188.6 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (23/29);  total (23/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
*Exported1,892,0192.C100.0% 189.6 / NA 52intergenic (‑437/+54)gudP/ygdLpredicted D‑glucarate transporter/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (23/29);  total (23/29)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891950‑1892077
                                                                      ||                                                          
aCTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag                                                             >  1:1154319/1‑70 (MQ=255)
aCTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag                                                             >  1:1159339/1‑70 (MQ=255)
 cTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag                                                             <  1:2208931/70‑2 (MQ=255)
    gCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct                                                           >  1:1103042/1‑66 (MQ=255)
          gCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca                                                    <  1:1697133/70‑1 (MQ=255)
           ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa                                                  <  1:2351477/71‑1 (MQ=255)
               aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg                                              >  1:197381/1‑71 (MQ=255)
                aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg                                             >  1:3223967/1‑71 (MQ=255)
                    attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:2359118/71‑1 (MQ=255)
                    attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:3833150/71‑1 (MQ=255)
                    attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         >  1:1944104/1‑71 (MQ=255)
                    attcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg                                         <  1:1741483/71‑1 (MQ=255)
                        gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      >  1:4132315/1‑70 (MQ=255)
                        gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      >  1:131093/1‑70 (MQ=255)
                        gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGAt                                      >  1:3644824/1‑70 (MQ=255)
                         attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:3137889/71‑1 (MQ=255)
                          ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                   <  1:4209194/71‑1 (MQ=255)
                          ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                   <  1:1772769/71‑1 (MQ=255)
                            cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa                                  >  1:1638793/1‑70 (MQ=255)
                             aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                <  1:1749050/71‑1 (MQ=255)
                             aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                >  1:3443538/1‑71 (MQ=255)
                             aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                >  1:1317496/1‑71 (MQ=255)
                             aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                <  1:298213/71‑1 (MQ=255)
                             aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg                                <  1:24685/71‑1 (MQ=255)
                              gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               <  1:2767483/71‑1 (MQ=255)
                              gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               <  1:3452346/71‑1 (MQ=255)
                              gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               <  1:1972842/71‑1 (MQ=255)
                               cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATGAGACgc                               >  1:3827123/1‑69 (MQ=255)
                               cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                   >  1:3575347/1‑66 (MQ=255)
                               cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt                              <  1:3982883/71‑1 (MQ=255)
                               cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               >  1:1619440/1‑70 (MQ=255)
                               cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt                               >  1:553395/1‑70 (MQ=255)
                                  cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATa                                     >  1:891440/1‑61 (MQ=255)
                                   gCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg                          <  1:2662413/71‑1 (MQ=255)
                                    cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTc                             >  1:2466187/1‑67 (MQ=255)
                                    cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt                         >  1:310342/1‑71 (MQ=255)
                                      aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa                                    <  1:1976264/58‑1 (MQ=255)
                                      aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg                                   >  1:1760021/1‑59 (MQ=255)
                                       aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:2082376/70‑1 (MQ=255)
                                       aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtct                       <  1:1988892/70‑1 (MQ=255)
                                       aaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc                        <  1:2239209/69‑1 (MQ=255)
                                        aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:2997915/44‑1 (MQ=255)
                                        aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:2216322/44‑1 (MQ=255)
                                        aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAc                                                <  1:2431102/44‑1 (MQ=255)
                                         aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                           >  1:4195979/1‑64 (MQ=255)
                                          cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATc                   <  1:2522928/71‑1 (MQ=255)
                                            aCATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc                  >  1:202204/1‑70 (MQ=255)
                                                 tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCAACCGACCt             >  1:136926/1‑70 (MQ=255)
                                                 tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAACACGTTCGCGTCTCATc                   >  1:1911112/1‑64 (MQ=255)
                                                     gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc                           <  1:4362729/52‑1 (MQ=255)
                                                     gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:1493834/71‑1 (MQ=39)
                                                     gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg        <  1:3450451/71‑1 (MQ=39)
                                                         gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt     <  1:3355806/70‑1 (MQ=38)
                                                           tGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt                              >  1:1613098/1‑43 (MQ=37)
                                                           tGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  >  1:1709172/1‑71 (MQ=39)
                                                             aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAc  <  1:3382678/69‑1 (MQ=38)
                                                                      ||                                                          
ACTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAC  >  Exported/1891950‑1892077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: