| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 30,273 | T→C | 6.5% | G593G (GGT→GGC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 30,273 | 0 | T | C | 6.5% | 169.7 / 3.9 | 61 | G593G (GGT→GGC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (27/30); new base C (2/2); total (29/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
AGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGC > Exported/30215‑30339 | aGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt > 1:2246741/1‑70 (MQ=255)aGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt > 1:4013542/1‑70 (MQ=255)aGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt < 1:2972580/70‑1 (MQ=255)aGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt > 1:16251/1‑70 (MQ=255) gggTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAtt < 1:1827662/70‑1 (MQ=255) gggTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTa < 1:1037913/71‑1 (MQ=255) ggcaTCGAGTTCGACTACTGCTGCGTGCACGCGGCGTTGGCGATGCGCGAAGACGGCTACGAGACcatcat < 1:3723251/68‑1 (MQ=255) gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGa < 1:2694759/56‑1 (MQ=255) gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGa < 1:1131595/56‑1 (MQ=255) gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTa > 1:1139172/1‑64 (MQ=255) gAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTaa > 1:2304589/1‑71 (MQ=255) aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAAc > 1:1682105/1‑71 (MQ=255) aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAAc > 1:2570048/1‑71 (MQ=255) aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAAc > 1:3545945/1‑71 (MQ=255) aaTTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAAc > 1:738083/1‑71 (MQ=255) gttCGACTACTGCTGCGTGCACGCCGCCCTCGCCATGCGCGAAGACGGCTACGAAACCATCATGGTCAACt < 1:4201379/70‑1 (MQ=255) ttCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTAc < 1:512885/51‑1 (MQ=255) ttCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTAc < 1:2591/51‑1 (MQ=255) ttCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAAc < 1:501100/56‑1 (MQ=255) ctGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAAcc < 1:912572/68‑1 (MQ=255) ctGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCg < 1:4101332/70‑1 (MQ=255) tGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCgg > 1:1705805/1‑70 (MQ=255) tGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCAgg > 1:3129122/1‑70 (MQ=255) ttGCGTACACGCCTCGCTGGCGCTGCGCGATGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGa < 1:2294315/69‑1 (MQ=255) ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAACCCATTATGGTTAACTGTAACCCGGaaa > 1:422740/1‑71 (MQ=255) ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGaa < 1:1694610/70‑1 (MQ=255) ttGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGa < 1:713255/69‑1 (MQ=255) ctGCGTGCACGCCGCCCTCGCCATGCGCGAAGACGGCTACGaaa > 1:1841063/2‑44 (MQ=12) ctGCGTGCACGCCGCCCTCGCCATGCGCGAAGACGGCTACGAAACCATCATGGTCAACTGCAACCCCGaga > 1:3010605/2‑69 (MQ=255) tGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGaa < 1:124875/69‑1 (MQ=255) gCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAAGTGTAACCCGGAAAc < 1:4293684/70‑1 (MQ=255) gCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAAc < 1:487222/70‑1 (MQ=255) cGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAAcc < 1:2772537/70‑1 (MQ=255) gTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGt < 1:188231/71‑1 (MQ=255) tACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGtc < 1:1708802/71‑1 (MQ=255) cacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGtctc < 1:609562/71‑1 (MQ=255) cacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGtctc < 1:3288903/71‑1 (MQ=255) cacGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGtctc < 1:2164209/71‑1 (MQ=255) gCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGtt < 1:1760819/45‑1 (MQ=255) tCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt < 1:329136/35‑1 (MQ=255) tCGCTGGCGCTGCGCGAAGACGGTTACGAAACCAt < 1:1930321/35‑1 (MQ=255) cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTaa > 1:2369383/1‑43 (MQ=255) cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTaa > 1:4412662/1‑43 (MQ=255) cGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGag > 1:1328076/1‑70 (MQ=255) gCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTaa > 1:4065620/1‑42 (MQ=255) gCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGAc > 1:2340269/1‑70 (MQ=255) ggCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGaaa > 1:3301842/1‑53 (MQ=255) ggCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTAc > 1:4109237/1‑70 (MQ=255) gcgcTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGa > 1:3133679/1‑71 (MQ=255) gcgcTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGa > 1:2703623/1‑71 (MQ=255) cgcgAAGACGGTTACGAAACCATTATGGTTAACTGTaa < 1:652843/38‑1 (MQ=255) cgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTcc > 1:70327/1‑70 (MQ=255) cgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTcc < 1:424676/70‑1 (MQ=255) cgAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTcc < 1:2103137/70‑1 (MQ=255) aGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGAcc > 1:2498252/1‑71 (MQ=255) aGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGAc > 1:1139407/1‑70 (MQ=255) gACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCg > 1:1071186/1‑71 (MQ=255) gACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCg > 1:3803574/1‑71 (MQ=255) gACGGTTACGAAACCATTATGGTTAACTGTAACACGGAAACCGTCTCCACCGACTACGACACTTCCGACCg > 1:3592400/1‑71 (MQ=255) aCGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGc < 1:4274364/71‑1 (MQ=255) cGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTAc < 1:2066685/55‑1 (MQ=255) | AGGGTATCGAATTCGACTACTGTTGCGTACACGCCTCGCTGGCGCTGCGCGAAGACGGTTACGAAACCATTATGGTTAACTGTAACCCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGC > Exported/30215‑30339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |