| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,518,856 | G→A | 7.2% | D73D (GAC→GAT) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,518,856 | 0 | G | A | 7.2% | 272.9 / 8.7 | 83 | D73D (GAC→GAT) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (46/31); new base A (4/2); total (50/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01 | |||||||||||
CAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTA > Exported/2518787‑2518926 | cAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2977191/1‑71 (MQ=255) aGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2964796/1‑70 (MQ=255) gCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCa < 1:1435286/71‑1 (MQ=255) gCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCa < 1:2189596/71‑1 (MQ=255) tCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATg > 1:3332155/1‑71 (MQ=255) cGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATg > 1:2715722/1‑70 (MQ=255) ggCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca < 1:605792/71‑1 (MQ=255) cGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgcag > 1:2694040/1‑70 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca > 1:3796510/1‑66 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCagcagc > 1:828202/1‑71 (MQ=255) cGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCagcagc > 1:52649/1‑71 (MQ=255) gCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAgcg > 1:940297/1‑71 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:1009818/1‑71 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:4001146/1‑71 (MQ=255) ccAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCAt > 1:3928624/1‑71 (MQ=255) tCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTc > 1:1291767/1‑68 (MQ=255) ccATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAg > 1:355024/1‑71 (MQ=255) cATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACg > 1:4266451/1‑37 (MQ=255) atatCCTGGTATGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc < 1:2124787/71‑1 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:3118645/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:436797/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:1637602/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:4058352/1‑37 (MQ=255) atatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGt > 1:2769226/1‑37 (MQ=255) tatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc > 1:3861253/1‑70 (MQ=255) tatCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTc > 1:3261600/1‑70 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgcagca > 1:728838/1‑43 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATgca > 1:2172637/1‑40 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGcagcag > 1:2469247/1‑44 (MQ=255) tCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGcagcag > 1:1308013/1‑44 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:3383147/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:471984/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:633174/1‑71 (MQ=255) ccTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:492457/1‑71 (MQ=255) ccTGGTAGGTGGAGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt > 1:2738902/1‑71 (MQ=255) cTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt < 1:945360/71‑1 (MQ=255) cTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt < 1:3573452/71‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:1956127/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:3272235/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCg < 1:2022786/55‑1 (MQ=255) tGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGtt > 1:3466994/1‑70 (MQ=255) gTAGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCg < 1:3813527/53‑1 (MQ=39) gTAGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCg < 1:2014347/53‑1 (MQ=39) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:4276813/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:3314495/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:2595588/69‑1 (MQ=255) gTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTc < 1:2848010/70‑1 (MQ=255) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3643209/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:985662/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3331001/1‑53 (MQ=39) aGGTGGCGGGTGTATCTTCAATCACATCATGCAGCAGCGCCGCCATCAGCGtt > 1:3182362/1‑53 (MQ=39) aGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAtc > 1:3493867/1‑71 (MQ=255) ggTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCAtct > 1:2268266/1‑71 (MQ=255) tGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:904977/64‑1 (MQ=255) tGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt < 1:3811393/64‑1 (MQ=255) ggCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCgg > 1:3122182/1‑71 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:716077/1‑64 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:2512466/1‑64 (MQ=255) gCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTAGAGTTTCa > 1:1914530/1‑64 (MQ=255) ggAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAg < 1:2001533/71‑1 (MQ=255) gTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCa < 1:239353/67‑1 (MQ=255) gTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCa < 1:892558/67‑1 (MQ=255) gTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCa < 1:3061575/67‑1 (MQ=255) gTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCa < 1:3176079/67‑1 (MQ=255) gTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAAt < 1:1700880/71‑1 (MQ=255) tATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGt < 1:1748317/53‑1 (MQ=255) tATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATg > 1:353537/1‑71 (MQ=255) tATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATg < 1:698745/71‑1 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGttt > 1:1365264/1‑53 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTc < 1:2796675/54‑1 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTc < 1:154886/54‑1 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTc < 1:2425740/54‑1 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATg > 1:60134/1‑69 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGc < 1:174514/70‑1 (MQ=255) tCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGc < 1:382476/70‑1 (MQ=255) cTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAAt > 1:1109435/1‑67 (MQ=255) cAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAg < 1:2034555/68‑1 (MQ=255) aaTCACGTCATGCAGCAGCGCCGCCATCAGCGttt > 1:3697887/1‑35 (MQ=255) aaTCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCa < 1:3772184/70‑1 (MQ=255) aaTCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCa < 1:3429017/70‑1 (MQ=255) aCGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCg > 1:1330608/1‑39 (MQ=255) aCGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:50352/1‑46 (MQ=255) aCGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCa > 1:316596/1‑46 (MQ=255) aCGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGc > 1:3623092/1‑71 (MQ=255) gTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTa > 1:753324/1‑71 (MQ=255) gTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTa > 1:3086410/1‑71 (MQ=255) gTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTa > 1:2774090/1‑71 (MQ=255) | CAGCTCGGCGACGCTTTTACCAAAAAGCTGTTCCATATCCTGGTAGGTGGCGGGAGTATCTTCAATCACGTCATGCAGCAGCGCCGCCATCAGCGTTTCATAGTCGAGTTTCATCTCGGCCAGAATGCAGGCAACCGCTA > Exported/2518787‑2518926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |