| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,567,260 | T→C | 5.9% | intergenic (‑209/+21) | mtlD ← / ← mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,567,260 | 0 | T | C | 5.9% | 343.9 / 11.1 | 118 | intergenic (‑209/+21) | mtlD/mtlA | mannitol‑1‑phosphate dehydrogenase, NAD(P)‑binding/fused mannitol‑specific PTS enzyme IIABC components |
| Reads supporting (aligned to +/- strand): ref base T (40/71); new base C (4/3); total (44/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
Only 100 of 127 total aligned reads displayed.
CAACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGT > Exported/2567190‑2567328 | caacaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAt > 1:1360046/1‑71 (MQ=255) aacaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtg < 1:781704/71‑1 (MQ=255) aacaaATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAt < 1:1475875/70‑1 (MQ=255) aaaTTTTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:2956612/71‑1 (MQ=255) aaTTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:3006938/71‑1 (MQ=255) tgtgCTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag > 1:3462540/1‑71 (MQ=255) tgtgATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag > 1:4409376/1‑71 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:3590455/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:2325380/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:1176472/70‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:298036/71‑1 (MQ=255) aTGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCCTCTCCACATGGAGAAGGt < 1:3091785/70‑1 (MQ=21) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGt < 1:3330848/69‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:2445853/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:1884574/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:3891042/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTgg < 1:876834/71‑1 (MQ=255) tGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:2762115/70‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCAtgtg < 1:4003552/59‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTggg < 1:282487/70‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGgga < 1:3358786/71‑1 (MQ=255) cATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGgga < 1:556498/71‑1 (MQ=255) aTCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggat < 1:1134733/71‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:4256397/58‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:866629/58‑1 (MQ=255) tCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTgg < 1:1227673/58‑1 (MQ=255) ccTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:226292/1‑71 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattgg < 1:1604559/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattgg < 1:45056/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattgg < 1:1041713/71‑1 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:1883784/1‑70 (MQ=255) cTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGggattg > 1:3163063/1‑70 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:544862/54‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGagag < 1:4592/54‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:1808628/71‑1 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:1983927/49‑1 (MQ=255) cTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGga < 1:1507705/49‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGGAATTAACCCCACCTTCTCCAt < 1:874399/43‑1 (MQ=255) tGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttactt < 1:1666934/69‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTAATCCCACCCTCTCCACATGgagaa > 1:977454/1‑49 (MQ=18) gATAAGCGTAGCGCATCAGGCAATTAATCCCACCCTCTCCACATGGAGAagg > 1:4367440/1‑52 (MQ=17) gATAAGCGTAGCGCATCAGGCAATTAATCACACCCTCTCCACATGGAGAagg > 1:3493153/1‑52 (MQ=17) caTAAGCGTAGCGCATCAGGCAATTAATCCCACCATCTCCACATGGAGAagg > 1:2055874/2‑52 (MQ=18) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTg < 1:4012893/53‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac < 1:2823364/69‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttacttac < 1:2134512/69‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttact < 1:633058/66‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:1377683/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:3929811/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACg < 1:3066857/70‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:2738317/1‑71 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:2847608/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:1307737/71‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa < 1:2284931/71‑1 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg > 1:1040382/1‑48 (MQ=255) aGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAggg > 1:607228/1‑48 (MQ=255) cGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGc < 1:3608707/71‑1 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttac > 1:2854844/1‑49 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGAttac > 1:3594054/1‑49 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGAc > 1:3279558/1‑56 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc < 1:3560561/70‑1 (MQ=255) aGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTc > 1:582964/1‑69 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTcc < 1:262613/69‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:2390378/71‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:927702/71‑1 (MQ=255) ggCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:2212452/71‑1 (MQ=255) gCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg > 1:676055/1‑70 (MQ=255) gCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:61595/70‑1 (MQ=255) aTTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt > 1:2306916/1‑71 (MQ=255) aaCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa > 1:4344839/1‑71 (MQ=255) aaCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCa > 1:2728458/1‑71 (MQ=255) aCCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCAt < 1:918401/71‑1 (MQ=255) cccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTc < 1:4164105/67‑1 (MQ=255) ccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATcc > 1:2539323/1‑70 (MQ=255) ccACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATcc > 1:1199828/1‑70 (MQ=255) cACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc < 1:1849186/71‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:2632494/1‑41 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGa > 1:1064229/1‑41 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCcagc < 1:3242524/50‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1770434/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1198366/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAg < 1:1547238/58‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACg < 1:2705384/71‑1 (MQ=255) aCCTTCTCCATGTGGAGAGGGTGGGATT‑GATTACTTACGACCTGCcagc < 1:2191409/49‑1 (MQ=255) ccTTCTCCATGTGGAGAGGGTGGGATTGGAttactta > 1:1029954/1‑37 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:4116137/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:637194/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:1621516/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:864015/1‑58 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACt < 1:1898190/60‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCAc < 1:1863717/68‑1 (MQ=255) cTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCt < 1:1195300/71‑1 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3746820/1‑56 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:3821952/1‑56 (MQ=255) tctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:4154295/1‑56 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGcc > 1:4298648/1‑42 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:4029507/1‑55 (MQ=255) ctcCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCa > 1:2225017/1‑55 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTggtggt < 1:3642522/71‑1 (MQ=255) cATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTggtggt < 1:3208455/71‑1 (MQ=255) | CAACAAATTGTGATGCATCCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTAACCCCACCTTCTCCATGTGGAGAGGGTGGGATTGGATTACTTACGACCTGCCAGCAGTTCCAGCACTTCATCCACGCTGGTGGT > Exported/2567190‑2567328 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |