| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,726,603 | A→C | 7.7% | L160L (CTT→CTG) | yheS ← | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,726,603 | 0 | A | C | 7.7% | 173.7 / 10.6 | 65 | L160L (CTT→CTG) | yheS | fused predicted transporter subunits and ATP‑binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (28/32); new base C (3/2); total (31/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
GAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAGTTGTTCATTGCT > Exported/2726537‑2726668 | gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:577013/68‑1 (MQ=255)gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:2919074/68‑1 (MQ=255)gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCaa < 1:1391996/68‑1 (MQ=255)gagatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt < 1:3586244/71‑1 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGtt < 1:915776/71‑1 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt > 1:777171/1‑70 (MQ=255) agatcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGt > 1:2544637/1‑70 (MQ=255) atcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa < 1:1432369/71‑1 (MQ=255) atcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa < 1:592740/71‑1 (MQ=255) tcgagGTGGTTGGTCGGTTCGTCGAGCAACAGCAGGTCGGACGGGCACATCAGCGCCTGCGCCAGGtt > 1:698128/1‑68 (MQ=12) tcgagGTGGTTGGTCGGTTCGTCGAGCAACAGCAGGTCGGACGGGCACATCAGCGCCTGCGCCAGGtt > 1:891876/1‑68 (MQ=12) tcgagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAg < 1:2313079/71‑1 (MQ=255) gagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:2821488/1‑70 (MQ=255) gagGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa < 1:3818389/70‑1 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa > 1:3172757/1‑71 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3978063/71‑1 (MQ=255) gtggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:2489140/71‑1 (MQ=255) ggtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1879184/1‑71 (MQ=255) gtTGGTCGGTTCGTCGAGCAGCAGCAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCg < 1:3835698/66‑1 (MQ=12) gtTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATAc < 1:3482446/71‑1 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:2321576/1‑61 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGa > 1:658986/1‑61 (MQ=255) aGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGc > 1:4308151/1‑70 (MQ=255) cgtcgAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTaa > 1:2524353/1‑51 (MQ=255) tcgAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCgg > 1:1448014/1‑70 (MQ=255) gAGCAGCAGCAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCGCATGCGCCAGCCGCCCGag > 1:1745326/1‑69 (MQ=255) gAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:53758/53‑1 (MQ=255) agcagcagcAGGTCGGAAGGGCACATCAGCGCCTGCGCCAGGTTCAGGCGCATGCGCCAGCCGCCCGagaa < 1:2994622/71‑1 (MQ=255) cagcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAAt < 1:3866905/71‑1 (MQ=255) agcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATc < 1:2414877/71‑1 (MQ=255) agcagcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATc < 1:457950/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:1044827/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:3975827/71‑1 (MQ=255) agcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACt < 1:1470357/71‑1 (MQ=255) gcaAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACtt < 1:1021921/71‑1 (MQ=255) aGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTAcc < 1:2741484/71‑1 (MQ=255) gTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCgg < 1:3578605/56‑1 (MQ=255) tCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACg < 1:2686455/38‑1 (MQ=255) tCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACg < 1:1957139/38‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3165588/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:679759/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa < 1:3807066/39‑1 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1769781/1‑41 (MQ=255) cTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:4431460/1‑41 (MQ=255) tGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCa > 1:2938612/1‑38 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1850160/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1746519/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:679216/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:1168636/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:3044434/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:2622069/1‑37 (MQ=255) aCGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATa > 1:3510947/1‑37 (MQ=255) cGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTc < 1:2746179/71‑1 (MQ=255) ggCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTcc < 1:840226/71‑1 (MQ=255) cAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAg < 1:3474123/71‑1 (MQ=255) aTCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAgttg < 1:3799255/71‑1 (MQ=255) aTCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAg > 1:3164248/1‑68 (MQ=255) cAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCa > 1:2301216/1‑65 (MQ=255) cAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAgttgtt > 1:2910513/1‑71 (MQ=255) aGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCAc > 1:1307499/1‑49 (MQ=255) aGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAgttgtt < 1:369061/70‑1 (MQ=255) aGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAgttgtt < 1:3423204/70‑1 (MQ=255) gcgcCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAGTTGTTCa < 1:4100001/71‑1 (MQ=255) tGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTAc > 1:3218208/1‑47 (MQ=255) tGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTAc > 1:1202679/1‑47 (MQ=255) tGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTAc > 1:2411131/1‑47 (MQ=255) ggCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATc > 1:608534/1‑39 (MQ=255) ggCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAGTTGTTCATTGCt < 1:2936268/69‑1 (MQ=255) aaGGTTAAGACGCATACGCCAGCCACCGGAAAAATCACTTAc > 1:1790822/1‑42 (MQ=255) | GAGATCGAGGTGGTTAGTCGGTTCGTCGAGCAGCAGCAAGTCTGAACGGCAAATCAGCGCCTGGGCAAGGTTAAGACGCATACGCCAGCCCCCGGAAAAATCACTTACCGGGCGCTCCAGTTGTTCATTGCT > Exported/2726537‑2726668 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |