Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 129,042 C→T 9.4% L201L (CTG→TTG)  yadH → predicted transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported129,0420CT 9.4% 298.7 / 16.8 96L201L (CTG→TTG) yadHpredicted transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (44/43);  new base T (7/2);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 1.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGT  >  Exported/128972‑129112
                                                                      |                                                                      
gtTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGc                                                                        >  1:4731050/1‑71 (MQ=255)
 ttAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGTGCt                                                                       <  1:5791042/71‑1 (MQ=255)
   aaCGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                                                                     >  1:5202279/1‑71 (MQ=255)
    aCGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTc                                                                    >  1:3928985/1‑71 (MQ=255)
    aCGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTc                                                                    >  1:6094576/1‑71 (MQ=255)
      gCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGc                                                                  <  1:5208421/71‑1 (MQ=255)
        cACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCAc                                                                >  1:5814470/1‑71 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCg                                                                   >  1:2807862/1‑63 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCg                                                                   >  1:5247363/1‑63 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCa                                                                 >  1:3803002/1‑65 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCa                                                                 >  1:4480308/1‑65 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCa                                                                 >  1:2095553/1‑65 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTg                                                             <  1:2970392/69‑1 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTg                                                             <  1:3754094/69‑1 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTg                                                             <  1:833012/69‑1 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                                                           >  1:894609/1‑71 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGaa                                                           >  1:1567577/1‑71 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                                                            <  1:1793161/70‑1 (MQ=255)
             aCGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                                                            <  1:2339001/70‑1 (MQ=255)
                  tttGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCa                                                       <  1:4848940/70‑1 (MQ=255)
                  tttGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGGCGCACCTGAACCCa                                                       <  1:4511321/70‑1 (MQ=255)
                       gCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTg                                                             <  1:1181270/59‑1 (MQ=255)
                          gggTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTta                                               >  1:6636012/1‑70 (MQ=255)
                            gTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                                                                     >  1:1757328/1‑46 (MQ=255)
                            gTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                                                                     >  1:679031/1‑46 (MQ=255)
                            gTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGt                                                                     >  1:6744026/1‑46 (MQ=255)
                             tCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATg                                           <  1:5602413/71‑1 (MQ=255)
                             tCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATg                                           <  1:127470/71‑1 (MQ=255)
                             tCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATg                                           <  1:3948811/71‑1 (MQ=255)
                             tCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATg                                           <  1:770664/71‑1 (MQ=255)
                               ttttACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGAt                                         >  1:26811/1‑71 (MQ=255)
                                 ttACTCACTGACTTTGTTGCCTCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTta                                               >  1:6491671/1‑63 (MQ=255)
                                 ttACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTtat                                              >  1:3385459/1‑64 (MQ=255)
                                       aCTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGa                                          >  1:6085462/1‑62 (MQ=255)
                                       aCTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTCAACCCAATCGTTTATATGa                                          >  1:6630227/1‑62 (MQ=255)
                                        cTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCa                                                                 >  1:1344770/1‑38 (MQ=255)
                                        cTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                                                            <  1:6797014/43‑1 (MQ=255)
                                        cTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                                                            <  1:5768278/43‑1 (MQ=255)
                                        cTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGa                                                            <  1:3254321/43‑1 (MQ=255)
                                        cTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGttt                                <  1:5732650/71‑1 (MQ=255)
                                         tGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTg                                                             <  1:1751150/41‑1 (MQ=255)
                                         tGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTc                               >  1:5183198/1‑71 (MQ=255)
                                         tGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTc                               >  1:879421/1‑71 (MQ=255)
                                         tGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTc                               >  1:4896888/1‑71 (MQ=255)
                                          gACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTta                                               >  1:5709878/1‑54 (MQ=255)
                                           aCTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCAcc                                                               >  1:1071789/1‑37 (MQ=255)
                                           aCTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTcc                              <  1:6175498/70‑1 (MQ=255)
                                           aCTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTcc                              <  1:748305/70‑1 (MQ=255)
                                               tgttgCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTAc                         >  1:1548724/1‑71 (MQ=255)
                                                 ttgCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAg                                      <  1:2803912/56‑1 (MQ=255)
                                                 ttgCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAg                                      <  1:1719963/56‑1 (MQ=255)
                                                 ttgCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAg                                      <  1:4008959/56‑1 (MQ=255)
                                                 ttgCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACg                        <  1:6939139/70‑1 (MQ=255)
                                                    ccgccgTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCtt                    >  1:2543662/1‑71 (MQ=255)
                                                    ccgccgTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCtt                    >  1:2697270/1‑71 (MQ=255)
                                                    ccgccgTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCt                     >  1:213349/1‑70 (MQ=255)
                                                    ccgccgTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCt                     >  1:2429654/1‑70 (MQ=255)
                                                     cgccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTAc                         <  1:726139/65‑1 (MQ=255)
                                                     cgccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTAc                         <  1:3902540/65‑1 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:5319514/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:574441/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:4187167/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:3827677/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:276419/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACgg                       >  1:3670548/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGTTGTCGCACCTGAACCCAATCGTTTATATGATCAGAGGTTTCCGCTACgg                       >  1:6907948/1‑65 (MQ=255)
                                                       ccgTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTcc                  <  1:2136397/70‑1 (MQ=255)
                                                         gTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCg               >  1:5061634/1‑71 (MQ=255)
                                                         gTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCg               >  1:5833965/1‑71 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:1059911/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:5094409/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:1836522/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:6873718/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:3730535/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGtt                                                 <  1:3856693/36‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:6160476/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:1421466/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:6400506/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:1613900/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:6505049/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:2321502/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:3685118/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:1914449/41‑1 (MQ=255)
                                                          ttCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTatat                                            <  1:1055744/41‑1 (MQ=255)
                                                            cTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCa                                       >  1:1933046/1‑44 (MQ=255)
                                                            cTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGc                            <  1:3815339/55‑1 (MQ=255)
                                                            cTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTa            >  1:194624/1‑71 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTa                          >  1:3434107/1‑55 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTa                          >  1:1605753/1‑55 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTa                          >  1:734039/1‑55 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTa                          >  1:2308103/1‑55 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTa                          >  1:6194900/1‑55 (MQ=255)
                                                              ggCAAGGGCTGTCGCACCTGAACCCAATAGTTTATATGATCAGTGGTTTCCGCTa                          >  1:6238347/1‑55 (MQ=255)
                                                               gCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCa         <  1:4656843/71‑1 (MQ=255)
                                                                  aGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTcc                  >  1:6854772/1‑59 (MQ=255)
                                                                  aGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAat       >  1:4975729/1‑70 (MQ=255)
                                                                  aGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAat       >  1:4991679/1‑70 (MQ=255)
                                                                      cTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGt  <  1:3291206/71‑1 (MQ=255)
                                                                      |                                                                      
GTTAACGCCACTCACGTATTTGGGCGGGGTCTTTTACTCACTGACTTTGTTGCCGCCGTTCTGGCAAGGGCTGTCGCACCTGAACCCAATCGTTTATATGATCAGTGGTTTCCGCTACGGCTTCCTCGGTATCAATGATGT  >  Exported/128972‑129112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: