| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 204,556 | A→G | 100% | intergenic (+156/‑28) | alaV → / → rrlH | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 204,556 | 0 | A | G | 94.5% | 383.7 / 10.0 | 127 | intergenic (+156/‑28) | alaV/rrlH | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base G (44/76); total (45/82) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 147 total aligned reads displayed.
TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > Exported/204495‑204622 | ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:4823404/1‑70 (MQ=255)ttGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAg > 1:1889226/1‑70 (MQ=255) tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa > 1:6433027/1‑70 (MQ=255) tGAAACACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa > 1:4158550/1‑70 (MQ=255) aaaCACTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAAc < 1:5343247/71‑1 (MQ=255) acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt > 1:6783157/1‑71 (MQ=255) acTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCt < 1:2613634/71‑1 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:1417136/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:4243040/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:6285938/1‑71 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt < 1:3579539/71‑1 (MQ=255) cTGAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:1935606/1‑71 (MQ=255) gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:6788065/61‑1 (MQ=255) gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:4244656/61‑1 (MQ=255) gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1367319/61‑1 (MQ=255) gAACAATGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:6842481/61‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTc < 1:4957162/69‑1 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg > 1:6010986/1‑71 (MQ=255) aacaatGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCgg > 1:6642615/1‑71 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCgaga < 1:3538069/54‑1 (MQ=11) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:4541075/54‑1 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:1694857/54‑1 (MQ=255) atgAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGa < 1:62478/54‑1 (MQ=255) agaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGa < 1:1175899/69‑1 (MQ=14) agaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGa < 1:1845015/69‑1 (MQ=14) agaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGa < 1:3355599/69‑1 (MQ=14) agaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGa < 1:2516585/69‑1 (MQ=14) agaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGa < 1:3346251/69‑1 (MQ=14) aaGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCtt > 1:6264187/1‑60 (MQ=255) aGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAgg > 1:834081/1‑71 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:5337827/71‑1 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:4223653/71‑1 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:5848954/71‑1 (MQ=21) gttgttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGt < 1:1446305/71‑1 (MQ=21) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg > 1:1657431/1‑64 (MQ=25) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg > 1:5922656/1‑64 (MQ=25) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTtgtg > 1:5821865/1‑64 (MQ=25) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:5175659/1‑69 (MQ=25) gttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTa < 1:70526/70‑1 (MQ=25) ttCGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt < 1:3183509/68‑1 (MQ=25) cGTGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGaaa > 1:2537168/1‑46 (MQ=255) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc < 1:6552441/71‑1 (MQ=21) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc < 1:1782857/71‑1 (MQ=21) tGAGTCTCTCAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGAc < 1:3102704/71‑1 (MQ=21) ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:3648590/61‑1 (MQ=25) ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2305865/61‑1 (MQ=25) ctcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:2984521/61‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:6627086/69‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1899010/69‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:4696313/69‑1 (MQ=25) tcAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:5879017/69‑1 (MQ=25) tcAAATGTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTG‑GGTTAAGCGACTAAGc > 1:894888/1‑67 (MQ=11) cAAATTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGc < 1:4350440/59‑1 (MQ=255) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa > 1:579186/1‑69 (MQ=25) aaaTTTTCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTa < 1:719438/69‑1 (MQ=25) tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt > 1:4166293/1‑71 (MQ=21) tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt > 1:1459078/1‑71 (MQ=21) tttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt > 1:782764/1‑71 (MQ=21) ttCGCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt > 1:2137459/1‑70 (MQ=25) tctcAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:5178439/67‑1 (MQ=255) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:2063124/68‑1 (MQ=25) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa < 1:1391900/70‑1 (MQ=25) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa < 1:228180/70‑1 (MQ=25) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa < 1:1047616/70‑1 (MQ=25) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa < 1:893578/70‑1 (MQ=25) gCAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGa < 1:4103624/70‑1 (MQ=25) cAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt < 1:853233/66‑1 (MQ=25) cAACACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGt < 1:2905056/66‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGtt < 1:3032957/66‑2 (MQ=255) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:314650/66‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTg < 1:4811176/66‑1 (MQ=25) aaCACGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGc > 1:6161150/1‑71 (MQ=21) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:3678636/55‑1 (MQ=255) cacGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:2287168/55‑1 (MQ=255) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:2978191/1‑40 (MQ=255) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:5058006/1‑40 (MQ=255) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGtt > 1:6300003/1‑40 (MQ=255) cGATGATGGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTg > 1:5540478/1‑71 (MQ=21) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:1297728/52‑1 (MQ=255) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:595839/52‑1 (MQ=255) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:2162668/52‑1 (MQ=255) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:6178198/52‑1 (MQ=255) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:2351014/52‑1 (MQ=255) atgatgGATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTgg > 1:2476370/1‑70 (MQ=25) tgatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGt < 1:4316881/51‑1 (MQ=255) gatggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAg > 1:6292866/1‑71 (MQ=21) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTGAGCg < 1:2620369/48‑1 (MQ=11) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1017298/48‑1 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:5585581/48‑1 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCg < 1:1997758/48‑1 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaga > 1:1089406/1‑50 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:1587475/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:2551934/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:5437559/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3601486/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3706940/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3917090/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:4183723/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:3919462/1‑52 (MQ=255) atggATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTaca > 1:602141/1‑52 (MQ=255) | TTGAAACACTGAACAACGAAAGTTGTTCGTGAGTCTCTCAAATTTTCGCAACACGATGATGAATCGCAAGAAACATCTTCGGGTTGTGAGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG > Exported/204495‑204622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |