Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,814,964 C→G 5.1% A71A (GCG→GCC)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,814,9640CG 5.1% 389.1 / 7.9 119A71A (GCG→GCC) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (57/55);  new base G (3/3);  total (60/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 139 total aligned reads displayed.

CAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  Exported/1814895‑1815026
                                                                     |                                                              
cAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTGCAGGTCGTTGTGTTTACCACCcg                                                               >  1:4087823/1‑71 (MQ=255)
cAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcg                                                               >  1:2812308/1‑71 (MQ=255)
  gCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:1049320/1‑71 (MQ=255)
       tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:4279085/1‑71 (MQ=255)
              agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:4963637/1‑71 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:2460648/70‑1 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:1708652/70‑1 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCAccc                                                                >  1:1364263/1‑49 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCAccc                                                                >  1:3751785/1‑49 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgc                                                          <  1:4536589/55‑1 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGaa                                           <  1:4904720/70‑1 (MQ=255)
                      tGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGt                                         >  1:6927302/1‑71 (MQ=255)
                         cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:2410880/71‑1 (MQ=255)
                         cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:6796990/71‑1 (MQ=255)
                         cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:2611455/71‑1 (MQ=255)
                          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTgg                                       >  1:5310989/1‑69 (MQ=255)
                          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGAAGCGTTGGGAAGTgg                                       >  1:2047235/1‑69 (MQ=255)
                            gcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgc                                   <  1:762287/71‑1 (MQ=255)
                             cgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgc                                   <  1:5189145/70‑1 (MQ=255)
                              ggTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgcgc                                 >  1:6274192/1‑71 (MQ=255)
                              agtgtAGCCGATGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:6027624/52‑1 (MQ=255)
                              agtgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:4039606/52‑1 (MQ=255)
                              agtgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:693214/52‑1 (MQ=255)
                               gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                >  1:2035440/1‑71 (MQ=255)
                               gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACACGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                >  1:3290043/1‑71 (MQ=255)
                                tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCgc                                                 >  1:5933786/1‑52 (MQ=255)
                                tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCg                                                  >  1:4721570/1‑52 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:6706853/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:772218/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:1440566/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:3609329/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:3584305/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:2569325/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:5084803/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:5104585/53‑1 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:5368208/1‑40 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:641579/1‑40 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCgg                               >  1:1956590/1‑70 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:2193344/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:3077360/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:6745771/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:870724/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCggg                              >  1:6191533/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGc                                                   >  1:5392485/1‑47 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:5267482/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6525830/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6901468/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:2769467/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:2810246/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:5966846/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:3306027/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:3458024/71‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:5396176/70‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:3151176/70‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:2317881/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:5000761/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:5100965/1‑70 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:1030993/71‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:6662543/71‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:5786401/71‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:2751974/71‑1 (MQ=255)
                                       gACGTTTTCCATGTAGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2549287/71‑1 (MQ=255)
                                        aCGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGa                                            <  1:653687/50‑1 (MQ=255)
                                        aCGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        >  1:4946847/1‑70 (MQ=255)
                                          gctttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4376835/63‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4441685/64‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:2849518/71‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:6309461/71‑1 (MQ=255)
                                            tttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGc                   >  1:5503595/1‑71 (MQ=255)
                                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        >  1:3627095/1‑65 (MQ=255)
                                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                  >  1:6867450/1‑71 (MQ=255)
                                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                  >  1:768313/1‑71 (MQ=255)
                                               ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCtt                 <  1:1205094/70‑1 (MQ=255)
                                                cAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTg                <  1:3861902/70‑1 (MQ=255)
                                                cAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGt               <  1:4984460/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGTGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:6218943/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:2443835/1‑70 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:948317/71‑1 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:5940682/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:1663899/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGa            <  1:5880571/71‑1 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGa            <  1:3188644/71‑1 (MQ=255)
                                                    tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAg           >  1:4168050/1‑71 (MQ=255)
                                                    tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCG‑GGGAATAATTACGCTTGTCGAg           >  1:3609793/1‑70 (MQ=255)
                                                     cGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGc          >  1:1313101/1‑71 (MQ=255)
                                                       ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2561030/55‑1 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGca                  >  1:464825/1‑59 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5219262/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:1059950/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:2654202/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:2617262/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGAGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5813680/1‑70 (MQ=255)
                                                         gtgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGATTAATTACGCTTGTCGAGccc        >  1:1595110/1‑69 (MQ=255)
                                                         gtgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5621764/1‑69 (MQ=255)
                                                          tgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCaa      >  1:6351743/1‑70 (MQ=255)
                                                           gtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAg     >  1:3514400/1‑70 (MQ=255)
                                                            tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAgg    <  1:3916275/70‑1 (MQ=255)
                                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCa       >  1:4265336/1‑65 (MQ=255)
                                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGaa  >  1:3667977/1‑70 (MQ=255)
                                                               accaccCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:5158313/47‑1 (MQ=255)
                                                                     |                                                              
CAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  Exported/1814895‑1815026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑