Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,257,303 T→C 6.7% A1280A (GCA→GCG rpoB ← RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,257,3030TC 6.7% 221.3 / 6.3 75A1280A (GCA→GCGrpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base T (24/46);  new base C (2/3);  total (26/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CTTGGTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAGCGGC  >  Exported/2257234‑2257372
                                                                     |                                                                     
cTTGGTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATg                                                                      >  1:6155351/1‑71 (MQ=255)
cTTGGTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATg                                                                      >  1:6414948/1‑71 (MQ=255)
cTTGGTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATg                                                                      >  1:6503226/1‑71 (MQ=255)
cTTGGTACGACCGTTCACGTCATCAGACTTAAAGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTAt                                                                       >  1:2153482/1‑70 (MQ=255)
   ggTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATg                                                                      <  1:2231398/68‑1 (MQ=255)
   ggTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGc                                                                     <  1:5052499/69‑1 (MQ=255)
   ggTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCtt                                                                   >  1:2704638/1‑71 (MQ=255)
    gTACGGCCGTTCACGTCGTCCGACTTCACAGTCAGCATTTCCTGCAGCACGTACGATGCGCCGTACGCTTc                                                                  <  1:4567885/71‑1 (MQ=255)
    gTACGGCCGTTCACGTCGTCCGACTTCACAGTCAGCATTTCCTGCAGCACGTACGATGCGCCGTACGCTTc                                                                  <  1:6517602/71‑1 (MQ=255)
        gACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgc                                                              <  1:4713960/71‑1 (MQ=255)
         aCCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgcg                                                             <  1:2091266/71‑1 (MQ=255)
         aCCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgcg                                                             <  1:1893919/71‑1 (MQ=255)
         aCCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgcg                                                             <  1:4609342/71‑1 (MQ=255)
         aCCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgcg                                                             <  1:3567336/71‑1 (MQ=255)
         aCCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAgcg                                                             <  1:6788604/71‑1 (MQ=255)
               cACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcaca                                                       >  1:1684002/1‑71 (MQ=255)
               cACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcaca                                                       >  1:6236544/1‑71 (MQ=255)
                aCGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCacac                                                      >  1:5849965/1‑71 (MQ=255)
                aCGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCacac                                                      >  1:2328898/1‑71 (MQ=255)
                  gTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACtt                                                    <  1:1450514/71‑1 (MQ=255)
                  gTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACtt                                                    <  1:2662935/71‑1 (MQ=255)
                   tcatcaGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGcc                                                           <  1:23217/63‑1 (MQ=255)
                   tcatcaGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACtt                                                    <  1:4950100/70‑1 (MQ=255)
                   tcatcaGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTc                                                   <  1:4325246/71‑1 (MQ=255)
                       caGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCa                                                                >  1:1885234/1‑54 (MQ=255)
                       caGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAtc                                               >  1:56921/1‑71 (MQ=255)
                       caGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAtc                                               >  1:5396724/1‑71 (MQ=255)
                       caGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAt                                                >  1:3375371/1‑70 (MQ=255)
                        aGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAtct                                              <  1:5962869/71‑1 (MQ=255)
                        aGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAtct                                              <  1:1047156/71‑1 (MQ=255)
                         gACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCa                                                                >  1:5643535/1‑52 (MQ=255)
                         gACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCa                                                 >  1:3766093/1‑67 (MQ=255)
                         gACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCAtctc                                             <  1:2928900/71‑1 (MQ=255)
                          aCTTCACAGTCAGCATTTCCTGCAGCACGTACGATGCGCCGTACGCTTCCAGTGCCCACACCTCCAtctca                                            >  1:3061008/1‑70 (MQ=255)
                          aCTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTcc                                            >  1:2436696/1‑71 (MQ=255)
                              aaCGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTccc                                           >  1:739154/1‑68 (MQ=255)
                              aaCGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTccc                                           >  1:2640109/1‑68 (MQ=255)
                              aaCGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCg                                         >  1:4887160/1‑70 (MQ=255)
                                 ggTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAAc                                     <  1:2409553/71‑1 (MQ=255)
                                 ggTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAAc                                     <  1:5675560/71‑1 (MQ=255)
                                  gTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTAt                                                                       <  1:6549631/36‑1 (MQ=255)
                                  gTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTAt                                                                       <  1:3379037/36‑1 (MQ=255)
                                  gTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTAt                                                                       <  1:5641914/36‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:6141491/71‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:3095674/71‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:6138719/71‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:5099284/71‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTACGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:4996574/71‑1 (MQ=255)
                                   tGAGCATTTCCTGCAGGGTGTATGCTGCCCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGc                                   <  1:2774900/71‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:1733276/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:1575967/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:1574160/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:3270436/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:3557245/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:3410065/48‑1 (MQ=255)
                                     aGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCcac                                                        <  1:3319609/48‑1 (MQ=255)
                                       cATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGac                               <  1:575601/71‑1 (MQ=255)
                                           tCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGaccacc                           >  1:2477290/1‑71 (MQ=255)
                                           tCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGaccac                            <  1:679712/70‑1 (MQ=255)
                                           tCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGaccac                            <  1:1502273/70‑1 (MQ=255)
                                             cTGCAGGGTGTATGCTGCGCCTTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGa                         <  1:2937981/71‑1 (MQ=255)
                                             cTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGa                         <  1:6746413/71‑1 (MQ=255)
                                               gCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAAc                       >  1:4022591/1‑71 (MQ=255)
                                                   ggTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGaaa                                      >  1:3776250/1‑52 (MQ=255)
                                                    gtgtATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACtgtg                   <  1:5761615/70‑1 (MQ=255)
                                                       tATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCtt               <  1:5297558/71‑1 (MQ=255)
                                                        aTGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCtt               <  1:4729662/70‑1 (MQ=255)
                                                         tgctgcGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACt                      >  1:2240748/1‑62 (MQ=255)
                                                           atgcGCCGTACGCTTCCAGTGCCCACACCTCCATCTCACCGAAGCGCTGGCCACCGAACTGGGCTTTaccg           >  1:3629628/2‑70 (MQ=255)
                                                            tgcGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGc                  <  1:1943303/63‑1 (MQ=255)
                                                             gcgcCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTg                                 <  1:3863064/47‑1 (MQ=255)
                                                             gcgcCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTg                                 <  1:5962598/47‑1 (MQ=255)
                                                             gcgcCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTg                                 <  1:5899884/47‑1 (MQ=255)
                                                             gcgcCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTg                                 <  1:5773669/47‑1 (MQ=255)
                                                               gcCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGaa                        >  1:6443311/1‑54 (MQ=255)
                                                                ccGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAg      <  1:6362503/71‑1 (MQ=255)
                                                                ccGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAg      <  1:2631247/71‑1 (MQ=255)
                                                                 cGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAg      <  1:3576339/70‑1 (MQ=255)
                                                                   tATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAGCgg   >  1:1841819/1‑71 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACtg                     <  1:5758977/51‑1 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACtg                     <  1:3136769/51‑1 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCtt               <  1:2320486/57‑1 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCtt               <  1:802076/57‑1 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCtt               <  1:803575/57‑1 (MQ=255)
                                                                     tGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAGCGgc  <  1:6934166/70‑1 (MQ=255)
                                                                     |                                                                     
CTTGGTACGACCGTTCACGTCATCAGACTTAACGGTGAGCATTTCCTGCAGGGTGTATGCTGCGCCGTATGCTTCCAGCGCCCACACTTCCATCTCCCCGAAACGCTGACCACCGAACTGTGCCTTACCACCCAGCGGC  >  Exported/2257234‑2257372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: