BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,359,867	G→A	52.9%	noncoding (1228/2905 nt)	rrlA ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,359,867	0	G	A	52.9%	31.6 / 268.7	190	noncoding (1228/2905 nt)	rrlA	23S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (58/26); new base A (50/49); total (115/75)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 190 total aligned reads displayed.

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGC  >  Exported/2359815‑2359932

cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca                                                 >  1:5796897/1‑71 (MQ=31)

 ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac                                                <  1:500881/71‑1 (MQ=32)

   gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg                                              >  1:1635997/1‑71 (MQ=255)

      ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:6789775/1‑71 (MQ=34)

      ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:6880224/1‑71 (MQ=34)

      ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:2382682/1‑71 (MQ=34)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           >  1:4268511/1‑70 (MQ=35)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt                                           <  1:840202/70‑1 (MQ=35)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:4067701/70‑1 (MQ=255)

       tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCAACCCTCACAGGCCACCTTcaca                                               >  1:3505045/1‑66 (MQ=11)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATTCCTCACAGCACACCTTCACAGGCtt                                          >  1:4087961/1‑69 (MQ=25)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                        <  1:3387253/71‑1 (MQ=255)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                        <  1:5107648/71‑1 (MQ=255)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                        <  1:4186845/71‑1 (MQ=255)

         tCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc                                        <  1:6708360/71‑1 (MQ=255)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                         >  1:3779070/1‑68 (MQ=35)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                         >  1:3951941/1‑68 (MQ=35)

           gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg                                      <  1:1844654/71‑1 (MQ=34)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa                                    <  1:6692325/70‑1 (MQ=35)

              aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc                                   >  1:5249157/1‑71 (MQ=34)

                  aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                 <  1:5076908/69‑1 (MQ=255)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               <  1:3458748/70‑1 (MQ=255)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               <  1:6345313/70‑1 (MQ=255)

                   tGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               <  1:591830/70‑1 (MQ=255)

                     tCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                            <  1:6305769/71‑1 (MQ=255)

                      cAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc                            >  1:1218241/1‑70 (MQ=35)

                      cAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                            >  1:6168817/1‑70 (MQ=255)

                      cAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                            >  1:1891344/1‑70 (MQ=255)

                      cAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc                            >  1:2281590/1‑70 (MQ=255)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcc                              <  1:5595732/67‑1 (MQ=11)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcc                              <  1:2718653/67‑1 (MQ=11)

                       aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa                          >  1:6449304/1‑71 (MQ=255)

                        gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                         >  1:5659938/1‑55 (MQ=35)

                        gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc                         >  1:1272127/1‑71 (MQ=255)

                         cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                        <  1:4987930/71‑1 (MQ=34)

                         cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc                        <  1:4604932/71‑1 (MQ=34)

                         cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                 <  1:56940/62‑1 (MQ=11)

                         cATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                 <  1:3031138/62‑1 (MQ=11)

                          aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGc                                 <  1:4488663/61‑1 (MQ=11)

                          aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       <  1:4967176/71‑1 (MQ=255)

                          aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc                       <  1:212719/71‑1 (MQ=255)

                           ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                      <  1:967710/71‑1 (MQ=34)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                     >  1:3543461/1‑71 (MQ=255)

                            tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCca                      >  1:1779347/1‑70 (MQ=255)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                     >  1:1365587/1‑70 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                     >  1:4789343/1‑70 (MQ=35)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:6955304/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:520430/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:6046441/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:282853/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:3200033/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:4760955/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:5534327/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:6191674/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca                                                 >  1:3370982/1‑42 (MQ=11)

                             cGCACTTCTGATACCTCCAGAATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa                     >  1:5326964/1‑70 (MQ=255)

                               cACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  <  1:4387181/71‑1 (MQ=34)

                               cACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  <  1:6019606/71‑1 (MQ=34)

                                aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                         >  1:2317727/1‑47 (MQ=38)

                                aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa                                         >  1:663137/1‑47 (MQ=38)

                                aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               <  1:3317575/57‑1 (MQ=11)

                                aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               <  1:1933364/57‑1 (MQ=11)

                                aCTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac                 <  1:2577454/71‑1 (MQ=255)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       <  1:5141697/48‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       <  1:3555295/48‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       <  1:801632/48‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       <  1:2184122/48‑1 (MQ=11)

                                 cTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       <  1:6763598/48‑1 (MQ=11)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg                                             >  1:5428053/1‑40 (MQ=14)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTCCCCAacaacag               >  1:2521646/1‑69 (MQ=11)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  >  1:6211333/1‑67 (MQ=255)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  >  1:4869159/1‑67 (MQ=255)

                                   tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  >  1:410865/1‑67 (MQ=255)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca              >  1:5348448/1‑70 (MQ=40)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca              <  1:5232463/70‑1 (MQ=40)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca              <  1:3808528/70‑1 (MQ=40)

                                    cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca              <  1:3062870/70‑1 (MQ=40)

                                    cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt                                >  1:1891506/1‑52 (MQ=11)

                                    cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc                               >  1:472674/1‑53 (MQ=11)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca              >  1:922961/1‑67 (MQ=40)

                                       aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgt          >  1:246685/1‑71 (MQ=255)

                                        tACCTCTAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:4037413/37‑1 (MQ=37)

                                        tACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:5846793/37‑1 (MQ=14)

                                        tACCTCCAGCATACCTCACAGTACACCTTCACAGGCt                                           <  1:3624654/37‑1 (MQ=14)

                                          ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc       >  1:1035532/1‑71 (MQ=255)

                                          ccTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTc       >  1:1413584/1‑71 (MQ=255)

                                           cTCCAGCATACCTCACAGTACACCTTCACAGGCTTACa                                       >  1:2104979/1‑38 (MQ=14)

                                           cTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa                  >  1:3131796/1‑59 (MQ=11)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                          >  1:436391/1‑49 (MQ=38)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac               >  1:4222687/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac               >  1:1011665/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac               >  1:6365402/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac               >  1:5542471/1‑60 (MQ=40)

                                             ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAacac               >  1:5991946/1‑60 (MQ=40)

                                              cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg   >  1:6623327/1‑71 (MQ=255)

                                              cAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTccc                             >  1:6705047/1‑45 (MQ=11)

                                               aGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:6569819/1‑71 (MQ=255)

                                               aGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:166243/1‑71 (MQ=255)

                                               aGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGc  >  1:5672033/1‑71 (MQ=255)

                                                  aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTa                          >  1:6080783/1‑44 (MQ=38)

CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGC  >  Exported/2359815‑2359932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`